Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HOO69_RS00785 Genome accession   NZ_CP053541
Coordinates   142547..144238 (-) Length   563 a.a.
NCBI ID   WP_171801083.1    Uniprot ID   A0AAE7DVZ4
Organism   Vibrio europaeus strain NPI-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 137547..149238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HOO69_RS00755 (HOO69_00760) rplS 138044..138397 (+) 354 WP_004742596.1 50S ribosomal protein L19 -
  HOO69_RS00760 (HOO69_00765) yacG 138744..138938 (-) 195 WP_069667595.1 DNA gyrase inhibitor YacG -
  HOO69_RS00765 (HOO69_00770) zapD 139001..139741 (-) 741 WP_004742598.1 cell division protein ZapD -
  HOO69_RS00770 (HOO69_00775) coaE 139776..140384 (-) 609 WP_069667594.1 dephospho-CoA kinase -
  HOO69_RS00775 (HOO69_00780) pilD 140386..141255 (-) 870 WP_171801081.1 A24 family peptidase Machinery gene
  HOO69_RS00780 (HOO69_00785) pilC 141313..142536 (-) 1224 WP_171801082.1 type II secretion system F family protein Machinery gene
  HOO69_RS00785 (HOO69_00790) pilB 142547..144238 (-) 1692 WP_171801083.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HOO69_RS00790 (HOO69_00795) pilA 144244..144678 (-) 435 WP_069667590.1 pilin Machinery gene
  HOO69_RS00795 (HOO69_00800) nadC 144921..145808 (-) 888 WP_069667589.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HOO69_RS00800 (HOO69_00805) ampD 145940..146494 (+) 555 WP_171801084.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HOO69_RS00805 (HOO69_00810) fldB 146772..147293 (-) 522 WP_171801085.1 flavodoxin FldB -
  HOO69_RS00810 (HOO69_00815) xerD 147456..148352 (+) 897 WP_171802358.1 site-specific tyrosine recombinase XerD -
  HOO69_RS00815 (HOO69_00820) - 148393..149145 (+) 753 WP_171382778.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62507.44 Da        Isoelectric Point: 5.3739

>NTDB_id=445040 HOO69_RS00785 WP_171801083.1 142547..144238(-) (pilB) [Vibrio europaeus strain NPI-1]
MLTNLPSILRQAELLDPVQEAQLVKLVKDTDLTVAEVIIEQGWLTQAELASQLSRIFALPIIDFANYDYQTVCHNLGLRE
LITRSRAVPIRQSEHQLLIAVTDPTNIAIEEEFRFATGKQIELVLADIKQLNGAVRRLYGSAICGEEASHAKELSEQELV
SLVELSNDEINPVEDLSSDHSPVSRYINQILLDAVRKQASDIHFEPYEASYRVRFRCDGILIETQQPSHTLSRRLSARLK
IIAKLDIAERRLPQDGRIKLQLSASTAVDIRISTLPTLWGEKIVLRLLDSSTAELNIQSLGYNQHQQALYQKALSKPQGM
ILMTGPTGSGKTVSLYAGLNMLNTPQVNIATAEDPVEINLAGVNQVQINPKIGLDFAQALRAFLRQDPDIVMVGEIRDFE
TAEIAVKAAQTGHLVLSTLHTNSAAESVVRLQHMGISPFNVSSSLNLIIAQRLARKLCNHCKQQQTLPETHCQQLELANN
TVVFAANEQGCSECNQGYFGRTGIYEVMQINDELVHVINQEADAKAIENVAIKQGMQTLKESGTEKLLAGVTSYQELQRV
LYL

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=445040 HOO69_RS00785 WP_171801083.1 142547..144238(-) (pilB) [Vibrio europaeus strain NPI-1]
ATGCTAACCAACCTACCCTCTATTTTGCGCCAAGCTGAGCTTCTTGACCCTGTTCAAGAAGCTCAGTTAGTTAAGTTGGT
TAAAGATACTGACCTTACCGTAGCCGAAGTCATAATAGAGCAAGGTTGGCTCACCCAAGCGGAGCTAGCCTCACAACTCT
CGCGAATTTTTGCCTTACCCATCATCGACTTTGCAAATTACGATTATCAAACGGTATGCCACAACTTAGGCCTGCGTGAA
CTGATCACCCGCTCCCGAGCAGTACCTATTAGGCAGAGTGAACACCAACTGCTTATCGCCGTTACCGACCCAACCAATAT
CGCAATCGAAGAAGAGTTTCGCTTTGCAACCGGAAAGCAAATTGAGCTAGTTTTAGCTGACATAAAGCAGCTCAATGGCG
CGGTACGTCGCTTGTATGGCAGCGCTATCTGTGGTGAAGAAGCGAGTCATGCAAAAGAGCTGAGCGAACAAGAGCTCGTC
AGCCTAGTCGAGCTCTCTAATGATGAGATTAACCCAGTCGAAGACCTGAGTAGTGATCACTCACCAGTCAGCCGTTATAT
CAATCAAATCTTATTAGATGCGGTTAGGAAGCAAGCTTCTGATATCCACTTTGAACCCTATGAAGCCTCTTATCGTGTGC
GCTTTCGCTGTGATGGCATTTTGATTGAGACTCAGCAACCTAGCCATACATTAAGTCGCCGTTTATCCGCAAGACTAAAG
ATCATCGCCAAACTCGATATTGCCGAGCGCCGCTTACCTCAAGATGGGCGCATCAAGCTTCAACTAAGTGCAAGTACCGC
TGTCGATATTCGTATTTCAACCCTACCAACTCTATGGGGCGAGAAGATCGTGCTCCGTCTTCTTGATAGCAGCACTGCCG
AGTTAAACATTCAATCACTAGGGTACAACCAACACCAACAAGCTTTGTATCAAAAGGCTTTGAGCAAGCCCCAAGGCATG
ATTTTGATGACAGGACCAACAGGCAGCGGTAAGACAGTCTCACTTTATGCGGGGTTAAACATGCTCAATACCCCACAAGT
GAACATCGCCACCGCAGAAGACCCGGTGGAAATTAATTTAGCGGGCGTTAACCAAGTTCAAATCAACCCTAAAATTGGAT
TAGACTTCGCCCAAGCGTTACGCGCCTTCCTACGCCAAGACCCGGACATTGTCATGGTTGGTGAGATACGCGACTTCGAA
ACTGCTGAAATCGCAGTCAAAGCGGCACAAACAGGTCACCTAGTGCTATCAACATTGCATACGAACTCGGCTGCCGAAAG
CGTCGTGCGCTTGCAACATATGGGCATCTCCCCATTCAATGTCTCTTCATCATTGAACTTAATCATTGCCCAGCGTTTGG
CACGAAAGCTGTGTAACCATTGTAAGCAGCAGCAAACCTTGCCCGAGACACATTGCCAACAACTGGAGTTAGCAAACAAC
ACCGTGGTTTTTGCTGCTAATGAGCAAGGGTGCAGTGAATGCAATCAAGGCTACTTTGGGCGAACCGGAATCTATGAAGT
GATGCAGATAAACGATGAGTTAGTGCACGTCATCAATCAAGAGGCAGATGCTAAAGCCATCGAAAACGTCGCGATAAAGC
AAGGTATGCAGACGCTTAAAGAATCTGGAACGGAGAAACTGCTCGCAGGAGTGACCAGCTACCAAGAGTTGCAACGTGTT
CTTTACTTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

64.121

100

0.641
  pilB Vibrio parahaemolyticus RIMD 2210633

61.837

100

0.622
  pilB Vibrio campbellii strain DS40M4

61.279

100

0.613
  pilB Acinetobacter baumannii D1279779

48.689

94.849

0.462
  pilB Legionella pneumophila strain ERS1305867

46.237

99.112

0.458
  pilB Acinetobacter baylyi ADP1

47.269

94.316

0.446
  pilF Neisseria gonorrhoeae MS11

44.361

94.494

0.419
  pilB Haemophilus influenzae 86-028NP

42.827

85.435

0.366
  pilB Haemophilus influenzae Rd KW20

42.268

86.146

0.364
  pilF Thermus thermophilus HB27

38.06

95.204

0.362