Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   HN015_RS03190 Genome accession   NZ_CP053421
Coordinates   663383..663808 (+) Length   141 a.a.
NCBI ID   WP_166481378.1    Uniprot ID   -
Organism   Pediococcus acidilactici strain PMC65     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 653226..708819 663383..663808 within 0


Gene organization within MGE regions


Location: 653226..708819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HN015_RS03110 (HN015_03125) - 653226..653915 (-) 690 WP_008842223.1 N-acetylmannosamine-6-phosphate 2-epimerase -
  HN015_RS03115 (HN015_03130) - 654311..655390 (-) 1080 WP_166481369.1 tyrosine-type recombinase/integrase -
  HN015_RS03120 (HN015_03135) - 655526..656527 (-) 1002 WP_166481370.1 Abi family protein -
  HN015_RS03125 (HN015_03140) - 657057..657254 (-) 198 WP_166481371.1 hypothetical protein -
  HN015_RS03130 (HN015_03145) - 657488..657838 (-) 351 WP_159218511.1 hypothetical protein -
  HN015_RS03135 (HN015_03150) - 657899..658306 (-) 408 WP_128212081.1 ImmA/IrrE family metallo-endopeptidase -
  HN015_RS03140 (HN015_03155) - 658315..658656 (-) 342 WP_002831842.1 helix-turn-helix domain-containing protein -
  HN015_RS03145 (HN015_03160) - 658913..659119 (+) 207 WP_159215549.1 hypothetical protein -
  HN015_RS03150 (HN015_03165) - 659109..659417 (-) 309 WP_166481373.1 hypothetical protein -
  HN015_RS03155 (HN015_03170) - 659476..659694 (+) 219 WP_002833157.1 helix-turn-helix domain-containing protein -
  HN015_RS03160 (HN015_03175) - 659879..660082 (-) 204 WP_029258004.1 hypothetical protein -
  HN015_RS03165 (HN015_03180) - 660140..660907 (+) 768 WP_166481374.1 phage antirepressor -
  HN015_RS03170 (HN015_03185) - 660920..661126 (+) 207 WP_166481375.1 hypothetical protein -
  HN015_RS03175 (HN015_03190) - 661393..662010 (-) 618 WP_159209346.1 hypothetical protein -
  HN015_RS03180 (HN015_03195) - 662236..662691 (+) 456 WP_166481376.1 chromosome segregation protein SMC -
  HN015_RS03185 (HN015_03200) - 662692..663390 (+) 699 WP_166481377.1 ERF family protein -
  HN015_RS03190 (HN015_03205) ssb 663383..663808 (+) 426 WP_166481378.1 single-stranded DNA-binding protein Machinery gene
  HN015_RS03195 (HN015_03210) - 663820..664497 (+) 678 WP_128472133.1 putative HNHc nuclease -
  HN015_RS03200 (HN015_03215) - 664487..664711 (+) 225 WP_128472134.1 hypothetical protein -
  HN015_RS03205 (HN015_03220) - 664715..664963 (-) 249 WP_128472135.1 hypothetical protein -
  HN015_RS03210 (HN015_03225) - 664997..665758 (+) 762 WP_128472136.1 conserved phage C-terminal domain-containing protein -
  HN015_RS03215 (HN015_03230) - 665739..666554 (+) 816 WP_166481379.1 ATP-binding protein -
  HN015_RS03220 (HN015_03235) - 666672..667304 (+) 633 WP_166481380.1 RNA polymerase subunit sigma-70 -
  HN015_RS03225 (HN015_03240) - 667285..667686 (+) 402 WP_166481381.1 RusA family crossover junction endodeoxyribonuclease -
  HN015_RS03230 (HN015_03245) - 667683..667847 (+) 165 WP_166481382.1 hypothetical protein -
  HN015_RS03235 (HN015_03250) - 667819..668187 (+) 369 WP_166481383.1 N-acetylmuramoyl-L-alanine amidase -
  HN015_RS03240 (HN015_03255) - 668272..668706 (+) 435 WP_166481384.1 ArpU family phage packaging/lysis transcriptional regulator -
  HN015_RS03245 (HN015_03260) - 669248..669400 (+) 153 WP_166481385.1 hypothetical protein -
  HN015_RS03250 (HN015_03265) - 669369..669881 (+) 513 WP_166481386.1 HNH endonuclease -
  HN015_RS03255 (HN015_03270) - 669979..670236 (+) 258 WP_166481387.1 hypothetical protein -
  HN015_RS03260 (HN015_03275) - 670427..670885 (+) 459 WP_166481388.1 phage terminase small subunit P27 family -
  HN015_RS03265 (HN015_03280) - 670888..672786 (+) 1899 WP_166481389.1 terminase large subunit -
  HN015_RS03270 (HN015_03285) - 672776..672970 (+) 195 WP_024863127.1 DUF1056 family protein -
  HN015_RS03275 (HN015_03290) - 672973..674166 (+) 1194 WP_166481390.1 phage portal protein -
  HN015_RS03280 (HN015_03295) - 674144..674893 (+) 750 WP_159209379.1 head maturation protease, ClpP-related -
  HN015_RS03285 (HN015_03300) - 674893..676125 (+) 1233 WP_166481391.1 phage major capsid protein -
  HN015_RS03290 (HN015_03305) - 676198..676530 (+) 333 WP_024863131.1 head-tail connector protein -
  HN015_RS03295 (HN015_03310) - 676520..676867 (+) 348 WP_171461264.1 phage head closure protein -
  HN015_RS03300 (HN015_03315) - 676870..677277 (+) 408 WP_166481393.1 HK97-gp10 family putative phage morphogenesis protein -
  HN015_RS03305 (HN015_03320) - 677277..677657 (+) 381 WP_166481394.1 DUF806 family protein -
  HN015_RS03310 (HN015_03325) - 677671..678375 (+) 705 WP_166481395.1 phage tail protein -
  HN015_RS03315 (HN015_03330) - 678452..678826 (+) 375 WP_159209317.1 phage tail assembly chaperone -
  HN015_RS03320 (HN015_03335) - 678871..679056 (+) 186 WP_053905877.1 hypothetical protein -
  HN015_RS03325 (HN015_03340) - 679086..683741 (+) 4656 WP_166481396.1 tape measure protein -
  HN015_RS03330 (HN015_03345) - 683818..685587 (+) 1770 WP_166481397.1 phage distal tail protein -
  HN015_RS03335 (HN015_03350) - 685608..687968 (+) 2361 WP_166481398.1 phage tail spike protein -
  HN015_RS03340 (HN015_03355) - 687970..691263 (+) 3294 WP_166481399.1 hypothetical protein -
  HN015_RS03345 (HN015_03360) - 691256..691483 (+) 228 WP_166481400.1 hypothetical protein -
  HN015_RS03350 (HN015_03365) - 691476..691685 (+) 210 WP_166481401.1 hypothetical protein -
  HN015_RS03355 (HN015_03370) - 691728..691976 (+) 249 WP_166481402.1 hypothetical protein -
  HN015_RS03360 (HN015_03375) - 691993..692352 (+) 360 WP_166481403.1 hypothetical protein -
  HN015_RS03365 (HN015_03380) - 692364..693527 (+) 1164 WP_166481404.1 GH25 family lysozyme -
  HN015_RS03370 (HN015_03385) - 693527..693790 (+) 264 WP_138492841.1 hemolysin XhlA family protein -
  HN015_RS03375 (HN015_03390) - 693800..694165 (+) 366 WP_159215953.1 phage holin -
  HN015_RS03380 (HN015_03395) map 694626..695408 (+) 783 WP_002830702.1 type I methionyl aminopeptidase -
  HN015_RS03385 (HN015_03400) - 696303..697151 (+) 849 WP_056985220.1 ROK family protein -
  HN015_RS03390 (HN015_03405) - 697132..697863 (+) 732 WP_008842377.1 sugar phosphate isomerase/epimerase family protein -
  HN015_RS03395 (HN015_03410) - 698116..699624 (+) 1509 WP_166481405.1 sodium:solute symporter -
  HN015_RS03400 (HN015_03415) - 699639..700556 (+) 918 WP_008842374.1 dihydrodipicolinate synthase family protein -
  HN015_RS03405 (HN015_03420) - 701326..701661 (+) 336 WP_005919288.1 hypothetical protein -
  HN015_RS03410 (HN015_03425) - 701821..702039 (+) 219 WP_166481406.1 KxYKxGKxW signal peptide domain-containing protein -
  HN015_RS03415 (HN015_03430) - 702866..708466 (-) 5601 WP_166481407.1 hypothetical protein -
  HN015_RS03420 (HN015_03435) - 708610..708819 (+) 210 WP_166481408.1 hypothetical protein -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15654.36 Da        Isoelectric Point: 6.3503

>NTDB_id=444862 HN015_RS03190 WP_166481378.1 663383..663808(+) (ssb) [Pediococcus acidilactici strain PMC65]
MINRTILIGRLTKDVEVRYTAKGDAVASFTVAVNRQFTNSQGEREADFINCVMWRKAAENFAKYTQKGSLVGIDGRIQTR
SYENQQGQRVYVTEVVADNFSLLDSKPKGNQQNNARQASTPGDPFANGGQSIDISDDDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=444862 HN015_RS03190 WP_166481378.1 663383..663808(+) (ssb) [Pediococcus acidilactici strain PMC65]
ATGATTAATCGAACAATACTAATAGGACGTTTAACTAAAGATGTTGAGGTTCGCTACACAGCTAAAGGCGATGCGGTAGC
TAGTTTTACCGTGGCAGTTAACCGACAGTTTACCAACTCACAGGGTGAACGTGAAGCGGATTTCATTAACTGCGTAATGT
GGCGGAAAGCGGCGGAAAACTTTGCCAAGTACACACAAAAAGGTTCGTTGGTAGGCATTGACGGTCGAATTCAAACCCGT
TCGTACGAAAATCAACAAGGACAACGAGTTTATGTAACTGAGGTTGTAGCTGATAACTTCTCGTTGCTAGATTCAAAACC
AAAAGGCAACCAGCAAAATAACGCACGGCAAGCATCAACGCCGGGAGATCCATTCGCCAATGGCGGACAGTCAATTGATA
TTAGTGACGACGATTTACCGTTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

59.412

100

0.716

  ssbA Bacillus subtilis subsp. subtilis str. 168

54.651

100

0.667

  ssbB Bacillus subtilis subsp. subtilis str. 168

62.037

76.596

0.475

  ssbB Streptococcus sobrinus strain NIDR 6715-7

42.254

100

0.426

  ssbB/cilA Streptococcus mitis NCTC 12261

42.254

100

0.426

  ssbB/cilA Streptococcus pneumoniae TIGR4

42.254

100

0.426

  ssbB/cilA Streptococcus pneumoniae Rx1

41.549

100

0.418

  ssbB/cilA Streptococcus pneumoniae D39

41.549

100

0.418

  ssbB/cilA Streptococcus pneumoniae R6

41.549

100

0.418

  ssbB/cilA Streptococcus mitis SK321

41.549

100

0.418

  ssbA Streptococcus mutans UA159

38.732

100

0.39