Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HKK52_RS25295 Genome accession   NZ_CP052862
Coordinates   5497736..5498953 (-) Length   405 a.a.
NCBI ID   WP_169373029.1    Uniprot ID   -
Organism   Pseudomonas sp. ADAK2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5492736..5503953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKK52_RS25255 (HKK52_25255) - 5493554..5493727 (-) 174 WP_054051322.1 DUF3094 family protein -
  HKK52_RS25260 (HKK52_25260) - 5493782..5494309 (-) 528 WP_169373025.1 MOSC domain-containing protein -
  HKK52_RS25265 (HKK52_25265) - 5494306..5494932 (-) 627 WP_008154887.1 DUF1780 domain-containing protein -
  HKK52_RS25270 (HKK52_25270) - 5495067..5495756 (+) 690 WP_169373026.1 energy-coupling factor ABC transporter permease -
  HKK52_RS25275 (HKK52_25275) - 5495820..5496035 (+) 216 WP_008033212.1 hypothetical protein -
  HKK52_RS25280 (HKK52_25280) yacG 5496045..5496245 (-) 201 WP_149661048.1 DNA gyrase inhibitor YacG -
  HKK52_RS25285 (HKK52_25285) coaE 5496242..5496865 (-) 624 WP_169373027.1 dephospho-CoA kinase -
  HKK52_RS25290 (HKK52_25290) pilD 5496862..5497734 (-) 873 WP_169373028.1 A24 family peptidase Machinery gene
  HKK52_RS25295 (HKK52_25295) pilC 5497736..5498953 (-) 1218 WP_169373029.1 type II secretion system F family protein Machinery gene
  HKK52_RS25300 (HKK52_25300) pilB 5498956..5500656 (-) 1701 WP_169373030.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HKK52_RS25305 (HKK52_25305) - 5500882..5501283 (+) 402 WP_169373031.1 pilin -
  HKK52_RS25310 (HKK52_25310) - 5501615..5501965 (+) 351 WP_169373032.1 BON domain-containing protein -
  HKK52_RS25315 (HKK52_25315) - 5502134..5502430 (-) 297 WP_169373033.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44165.08 Da        Isoelectric Point: 10.0532

>NTDB_id=441923 HKK52_RS25295 WP_169373029.1 5497736..5498953(-) (pilC) [Pseudomonas sp. ADAK2]
MAVKAAKISVYAWEGMDKKGTKMSGELSGLNPALIKAQLRKQGINPGKVRKKSASIFSLGKRIKAQDISLFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDEVKQEVAAGNSFAASLRKKPQYFDELYCNLVDAGEQAGALDTLLDRVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFKTVFAGFGAELPGFTVMVISLSEFMQEWWWVMLGALAAAIFGVR
RAFKKSQTFRDRVDTWLLKVPLVGTLMYKSAVARYARTLSTTFAAGVPLVDALESVAGATGNIVFKRAVLRIRQDVSTGM
QLNFAMRTSGVFPNMAIQMAAIGEESGALDDMLDKVASFYEDEVDNMVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=441923 HKK52_RS25295 WP_169373029.1 5497736..5498953(-) (pilC) [Pseudomonas sp. ADAK2]
ATGGCGGTCAAGGCAGCAAAAATCAGCGTCTACGCCTGGGAAGGCATGGACAAGAAAGGCACGAAAATGAGCGGCGAGCT
GAGCGGCCTGAACCCGGCGCTGATCAAGGCACAGCTACGCAAGCAAGGGATTAACCCGGGCAAGGTGCGCAAGAAGTCCG
CGTCGATTTTCAGCCTGGGCAAACGCATCAAGGCCCAGGACATTTCTTTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGTTGCTGCAGTCGTTCGACATCATTGGCGAAGGCTTCGACAACCCGGCCATGCGCAAGCTGGTGGATGA
GGTGAAACAGGAAGTTGCAGCCGGAAACAGCTTCGCCGCCTCTTTGCGCAAGAAACCGCAATATTTCGACGAGTTGTACT
GCAACCTGGTGGACGCCGGTGAACAGGCCGGGGCCCTGGATACGCTGCTGGATCGGGTGGCGACCTATAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCGCTGGCCGTGGTGTTCGTCGCGATCATCGTCACCGGGAT
TCTGCTGGTCAAGGTGGTGCCGCAGTTCAAGACCGTGTTCGCCGGGTTTGGCGCCGAGCTGCCGGGTTTCACGGTGATGG
TCATCAGCCTGTCGGAGTTCATGCAGGAATGGTGGTGGGTCATGCTCGGCGCGCTGGCCGCAGCGATCTTCGGTGTGCGT
CGCGCCTTCAAAAAATCCCAGACCTTTCGCGATCGGGTGGACACCTGGCTGTTGAAAGTGCCCTTGGTCGGCACGCTGAT
GTACAAGTCCGCCGTCGCCCGCTATGCCCGCACGCTATCGACCACATTCGCCGCCGGGGTGCCGCTGGTGGATGCCCTGG
AATCGGTAGCCGGCGCTACCGGCAATATTGTGTTCAAGCGCGCGGTGCTGCGCATCAGGCAGGACGTGTCGACCGGCATG
CAGCTGAATTTCGCCATGCGCACCTCCGGCGTGTTCCCGAACATGGCGATCCAGATGGCGGCCATTGGCGAAGAGTCCGG
CGCCCTGGACGATATGCTCGACAAGGTCGCCAGCTTCTACGAGGACGAAGTCGACAACATGGTCGACAACCTCACCAGCC
TGATGGAACCGTTCATCATGGTGGTACTGGGTGTTGTCGTCGGCGGCCTGGTGGTTGCGATGTACCTGCCCATTTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.58

100

0.736
  pilC Acinetobacter baylyi ADP1

60.294

100

0.607
  pilC Acinetobacter baumannii D1279779

60.049

100

0.605
  pilC Legionella pneumophila strain ERS1305867

53.788

97.778

0.526
  pilG Neisseria gonorrhoeae MS11

44.417

99.506

0.442
  pilG Neisseria meningitidis 44/76-A

43.672

99.506

0.435
  pilC Vibrio cholerae strain A1552

42.469

100

0.425
  pilC Vibrio campbellii strain DS40M4

41.133

100

0.412
  pilC Thermus thermophilus HB27

36.658

99.012

0.363