Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HG264_RS09175 Genome accession   NZ_CP051625
Coordinates   1998557..1999822 (+) Length   421 a.a.
NCBI ID   WP_169407375.1    Uniprot ID   A0A7Z2V3E1
Organism   Pseudomonas sp. gcc21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1993557..2004822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HG264_RS09150 (HG264_09145) - 1993710..1994186 (+) 477 WP_256663651.1 transposase -
  HG264_RS09155 (HG264_09150) - 1994700..1995098 (+) 399 WP_169407371.1 GFA family protein -
  HG264_RS09160 (HG264_09155) - 1995289..1995837 (+) 549 WP_169407372.1 transposase -
  HG264_RS09165 (HG264_09160) pilA 1995992..1996486 (-) 495 WP_169407373.1 pilin Machinery gene
  HG264_RS09170 (HG264_09165) pilB 1996850..1998553 (+) 1704 WP_169407374.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HG264_RS09175 (HG264_09170) pilC 1998557..1999822 (+) 1266 WP_169407375.1 type II secretion system F family protein Machinery gene
  HG264_RS09180 (HG264_09175) pilD 1999826..2000698 (+) 873 WP_169407376.1 A24 family peptidase Machinery gene
  HG264_RS09185 (HG264_09180) coaE 2000700..2001305 (+) 606 WP_169407377.1 dephospho-CoA kinase -
  HG264_RS09190 (HG264_09185) yacG 2001302..2001493 (+) 192 WP_150303146.1 DNA gyrase inhibitor YacG -
  HG264_RS09195 (HG264_09190) - 2001512..2002456 (+) 945 WP_169407378.1 Nudix family hydrolase -
  HG264_RS09200 (HG264_09195) ispH 2002700..2003647 (-) 948 WP_169407379.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  HG264_RS09205 (HG264_09200) fkpB 2003678..2004115 (-) 438 WP_169407380.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  HG264_RS09210 (HG264_09205) lspA 2004283..2004777 (-) 495 WP_169407381.1 signal peptidase II -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 45907.88 Da        Isoelectric Point: 10.0115

>NTDB_id=439939 HG264_RS09175 WP_169407375.1 1998557..1999822(+) (pilC) [Pseudomonas sp. gcc21]
MAQQAAALKRKPAPAKKKVKEPKIYPFKWEGKDRKGTKISGEIQGSNPALIKAQLRKQGILVTKINKSSTLFGKRSKAIK
PLDIAFFTRQLATMMESGVPIVQAFEIIAEGSENPSVAKLVTTIKTDVAAGNTLADSLRQHPKYFDDLFCNLVESGEQSG
RLESLLDRIATYKEKTEALKAKIKKAMTYPIAVVVVAIVVTAILLLKVVPQFKEVFSSFGAELPAFTLFVIGLSEWLQDW
WFIILIGLIALGYAYTQVNRRSLKFRDAQDRALLKAPIVGKIIYEAAVARYARTLSTTFAAGVPLVDALDSVGGAVGNVV
FRNAVMKVKEDVSAGSQLNFSMRTTNVFPSLAVQMAGIGEESGNLDGMLEKVADYYEAEVDNKVDNLTTLLEPLIMSVLG
VLVGGLIIAMYLPIFQLGSVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=439939 HG264_RS09175 WP_169407375.1 1998557..1999822(+) (pilC) [Pseudomonas sp. gcc21]
ATGGCTCAGCAAGCAGCAGCGCTAAAAAGGAAGCCGGCCCCGGCGAAGAAGAAGGTAAAAGAACCCAAGATCTACCCCTT
CAAATGGGAGGGAAAGGATCGCAAGGGCACCAAGATTTCCGGCGAAATTCAGGGTTCGAACCCGGCCTTGATCAAGGCCC
AGCTGCGCAAGCAGGGCATTCTCGTTACCAAGATCAACAAATCCTCCACTTTGTTCGGCAAACGTAGCAAAGCGATCAAG
CCGCTTGATATCGCTTTTTTTACACGTCAGCTGGCGACCATGATGGAGTCCGGCGTACCGATTGTGCAGGCCTTCGAGAT
TATCGCCGAGGGCTCTGAAAATCCCAGCGTTGCGAAGCTTGTTACGACCATCAAGACAGATGTAGCTGCCGGTAATACGT
TGGCGGACTCCCTGCGTCAGCATCCCAAATATTTCGACGACCTGTTCTGTAACCTGGTTGAGTCCGGTGAGCAGTCAGGC
CGGCTGGAATCGCTGCTGGATCGGATCGCGACCTATAAGGAAAAGACCGAAGCACTCAAGGCCAAGATCAAGAAGGCCAT
GACCTATCCAATCGCGGTGGTTGTCGTTGCCATCGTGGTAACGGCTATCCTGCTGTTGAAGGTGGTTCCACAATTCAAGG
AAGTGTTCTCCAGCTTCGGCGCCGAGCTGCCGGCCTTTACCCTCTTCGTTATCGGCTTGTCAGAATGGCTGCAGGACTGG
TGGTTCATCATCCTGATAGGGCTTATTGCTCTGGGCTATGCATACACCCAGGTTAACCGACGATCGCTCAAGTTCCGGGA
CGCGCAGGACCGGGCATTACTCAAAGCCCCGATCGTCGGGAAGATCATCTACGAGGCAGCAGTGGCCCGTTACGCCCGCA
CCCTCTCAACCACCTTCGCCGCCGGTGTACCGCTTGTGGATGCGCTCGATTCGGTCGGTGGTGCGGTGGGGAACGTAGTG
TTCCGCAACGCAGTGATGAAGGTAAAAGAAGATGTGTCAGCCGGTTCACAGCTGAACTTCTCCATGCGTACTACCAACGT
GTTCCCTTCGCTCGCTGTGCAAATGGCTGGCATCGGTGAAGAATCCGGTAACCTCGACGGCATGCTTGAGAAGGTCGCGG
ACTATTACGAGGCCGAGGTGGATAACAAGGTCGACAACCTGACCACGCTGCTTGAGCCTCTGATCATGAGTGTTCTCGGC
GTTCTGGTCGGCGGTCTGATCATCGCCATGTACCTGCCAATCTTCCAGCTGGGCTCTGTTGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(292-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2V3E1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

69.114

93.824

0.648
  pilC Acinetobacter baylyi ADP1

59.753

96.2

0.575
  pilC Acinetobacter baumannii D1279779

59.36

96.437

0.572
  pilC Legionella pneumophila strain ERS1305867

53.616

95.249

0.511
  pilG Neisseria meningitidis 44/76-A

43.182

94.062

0.406
  pilG Neisseria gonorrhoeae MS11

42.929

94.062

0.404
  pilC Vibrio campbellii strain DS40M4

41.975

96.2

0.404
  pilC Vibrio cholerae strain A1552

41.919

94.062

0.394