Detailed information NTG0439940

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HG264_RS09180
• Genome accession: NZ_CP051625
• Coordinates: 1999826..2000698 (+)
• Length: 290 a.a.
• NCBI ID: WP_169407376.1
• Uniprot ID: A0A7Z2V3X2
• Organism: Pseudomonas sp. gcc21
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1994826..2005698

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HG264_RS09160 (HG264_09155)	-	1995289..1995837 (+)	549	WP_169407372.1	transposase	-
HG264_RS09165 (HG264_09160)	pilA	1995992..1996486 (-)	495	WP_169407373.1	pilin	Machinery gene
HG264_RS09170 (HG264_09165)	pilB	1996850..1998553 (+)	1704	WP_169407374.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HG264_RS09175 (HG264_09170)	pilC	1998557..1999822 (+)	1266	WP_169407375.1	type II secretion system F family protein	Machinery gene
HG264_RS09180 (HG264_09175)	pilD	1999826..2000698 (+)	873	WP_169407376.1	A24 family peptidase	Machinery gene
HG264_RS09185 (HG264_09180)	coaE	2000700..2001305 (+)	606	WP_169407377.1	dephospho-CoA kinase	-
HG264_RS09190 (HG264_09185)	yacG	2001302..2001493 (+)	192	WP_150303146.1	DNA gyrase inhibitor YacG	-
HG264_RS09195 (HG264_09190)	-	2001512..2002456 (+)	945	WP_169407378.1	Nudix family hydrolase	-
HG264_RS09200 (HG264_09195)	ispH	2002700..2003647 (-)	948	WP_169407379.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
HG264_RS09205 (HG264_09200)	fkpB	2003678..2004115 (-)	438	WP_169407380.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
HG264_RS09210 (HG264_09205)	lspA	2004283..2004777 (-)	495	WP_169407381.1	signal peptidase II	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32032.01 Da
• Isoelectric Point: 7.0272

>NTDB_id=439940 HG264_RS09180 WP_169407376.1 1999826..2000698(+) (pilD) [Pseudomonas sp. gcc21]
MTLIDYLASHVLAFVLLAGLLGLVIGSFLNVVIHRLPRMMERDWRIQAREILEPETEHPAEPTYNLVLPHSHCPHCQTEI
KAWQNVPVVSYVFLRGRCAQCKTRISPRYPLVELLTAVLSMVVAWQLGFGWAAAGLIFLTWGLIALSLIDADTQLLPDVI
VLPLLWLGLIVNSFGVYTDLSTALWGAVFGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVLPLTILLSSLV
GAILGIIILKSRGDSNATPLPFGPYLAIAGWIALIWGDTITGTYLRFAGL

Nucleotide

• Length: 873 bp

>NTDB_id=439940 HG264_RS09180 WP_169407376.1 1999826..2000698(+) (pilD) [Pseudomonas sp. gcc21]
ATGACTCTTATCGATTACCTGGCCAGCCACGTGCTGGCCTTTGTTTTGCTCGCGGGACTGCTGGGTTTGGTGATCGGCAG
TTTTCTCAACGTTGTGATACATCGCCTGCCCCGGATGATGGAGCGGGACTGGCGTATTCAGGCACGGGAAATTCTCGAGC
CTGAGACGGAACACCCCGCTGAGCCCACTTATAATCTCGTTCTACCCCATTCCCACTGCCCCCATTGCCAGACCGAAATC
AAAGCCTGGCAAAACGTGCCAGTGGTGAGCTATGTATTCCTGCGCGGCCGCTGCGCACAGTGCAAGACCCGGATCAGCCC
GCGCTATCCGCTGGTTGAGCTGCTGACCGCGGTCCTATCAATGGTCGTTGCCTGGCAGCTTGGCTTCGGCTGGGCCGCGG
CTGGCCTGATTTTCCTGACATGGGGCTTGATTGCCCTGAGCCTGATCGATGCCGACACCCAGTTGTTGCCTGATGTAATC
GTTCTGCCGCTGTTATGGCTGGGGCTTATCGTTAACAGCTTCGGTGTCTATACCGACCTCAGCACCGCTCTGTGGGGTGC
CGTGTTCGGCTACCTGAGCCTGTGGTCGGTGTACTGGCTGTTCAAACTGGTTACCGGCAAGGAAGGCATGGGTTACGGCG
ACTTCAAGCTGCTGGCCATGCTCGGCGCCTGGGGTGGCTGGCAAGTTCTGCCGCTCACCATTCTGCTTTCTTCTCTGGTG
GGCGCAATCCTCGGCATCATCATCCTGAAATCCCGCGGCGATTCCAACGCGACCCCCCTGCCCTTCGGGCCCTATCTGGC
GATTGCCGGGTGGATTGCCTTGATCTGGGGTGACACAATTACCGGCACCTATCTCAGATTCGCCGGATTATAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (138-246)

3D structure

• AlphaFold DB: A0A7Z2V3X2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.945	94.138	0.517
pilD	Acinetobacter baumannii D1279779	52.297	97.586	0.51
pilD	Acinetobacter nosocomialis M2	51.943	97.586	0.507
pilD	Vibrio campbellii strain DS40M4	51.648	94.138	0.486
pilD	Neisseria gonorrhoeae MS11	51.88	91.724	0.476