Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HG264_RS09170 Genome accession   NZ_CP051625
Coordinates   1996850..1998553 (+) Length   567 a.a.
NCBI ID   WP_169407374.1    Uniprot ID   A0A7Z2V3N2
Organism   Pseudomonas sp. gcc21     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1991850..2003553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HG264_RS09145 (HG264_09140) - 1992254..1993375 (-) 1122 WP_169407370.1 Fic family protein -
  HG264_RS09150 (HG264_09145) - 1993710..1994186 (+) 477 WP_256663651.1 transposase -
  HG264_RS09155 (HG264_09150) - 1994700..1995098 (+) 399 WP_169407371.1 GFA family protein -
  HG264_RS09160 (HG264_09155) - 1995289..1995837 (+) 549 WP_169407372.1 transposase -
  HG264_RS09165 (HG264_09160) pilA 1995992..1996486 (-) 495 WP_169407373.1 pilin Machinery gene
  HG264_RS09170 (HG264_09165) pilB 1996850..1998553 (+) 1704 WP_169407374.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HG264_RS09175 (HG264_09170) pilC 1998557..1999822 (+) 1266 WP_169407375.1 type II secretion system F family protein Machinery gene
  HG264_RS09180 (HG264_09175) pilD 1999826..2000698 (+) 873 WP_169407376.1 A24 family peptidase Machinery gene
  HG264_RS09185 (HG264_09180) coaE 2000700..2001305 (+) 606 WP_169407377.1 dephospho-CoA kinase -
  HG264_RS09190 (HG264_09185) yacG 2001302..2001493 (+) 192 WP_150303146.1 DNA gyrase inhibitor YacG -
  HG264_RS09195 (HG264_09190) - 2001512..2002456 (+) 945 WP_169407378.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62635.88 Da        Isoelectric Point: 5.0759

>NTDB_id=439938 HG264_RS09170 WP_169407374.1 1996850..1998553(+) (pilB) [Pseudomonas sp. gcc21]
MDTPALSGLARRIVQDQLLDATVASKASKQATLDKIPLITYLVQNKLGNARDLAMVCAEEFGYPFFDLAVLDKDHQPQDL
VSEKLIRQHCVLPLYKRGSRLFLAMSDPTNQQALSDIQFNTGLMTDAVMVEDDKLQAAIEKYLESPTGGLGDMDDSALDD
LDVESVNDDDKPTTSGGEADDAPIVRFVNKMLLDAVRMGSSDLHFEPYEKLYRVRFRTDGILHEVAKPPVQLGVRIAARL
KVMSSMDMAERRKPQDGRIKMKISRNKSIDFRVNTLPTLWGEKVVLRILDAESAKMGIDALGYEEDQKEMYLTALARPQG
MILVTGPTGSGKTVSLYTGLNILNTMERNISTAEDPVEINLEGINQVNVNPKQGLDFAKALRAFLRQDPDIIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARRLCKSCKAPFEVPRETLIEEGFSP
EKIDAGLTIYGPVGCENCKDGYKGRVGIYEVVKITPAMQRIIMEDGNSIQISDVAQQEGFRSLRQSALMKAEQGVTSLAE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=439938 HG264_RS09170 WP_169407374.1 1996850..1998553(+) (pilB) [Pseudomonas sp. gcc21]
ATGGATACCCCCGCGCTCTCTGGTCTGGCACGAAGGATCGTTCAGGATCAGTTGCTGGACGCAACCGTTGCCAGTAAGGC
CAGCAAACAGGCCACGCTAGACAAGATTCCCCTCATTACCTATCTGGTGCAGAACAAGCTGGGCAATGCACGCGACCTGG
CCATGGTCTGCGCCGAGGAATTCGGCTACCCGTTTTTTGATCTTGCGGTGCTGGACAAAGACCATCAGCCGCAGGATCTG
GTCAGCGAGAAGCTGATTCGCCAGCACTGCGTGCTGCCTCTTTATAAGCGTGGCTCGCGCCTGTTCCTGGCCATGTCCGA
TCCGACAAACCAGCAGGCGTTGAGCGATATCCAGTTCAACACCGGCTTGATGACCGATGCGGTCATGGTTGAGGACGACA
AGCTGCAGGCTGCGATCGAGAAGTATCTTGAAAGCCCGACTGGCGGGCTTGGGGATATGGATGATTCGGCGTTGGATGAT
CTGGACGTCGAGTCAGTCAACGATGATGACAAGCCCACGACCAGCGGCGGCGAGGCTGACGACGCGCCCATCGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCGGTACGCATGGGCTCATCGGACTTGCACTTCGAGCCCTATGAAAAACTTTATCGCG
TACGCTTCCGTACTGACGGCATTCTGCACGAGGTCGCGAAACCTCCAGTGCAGCTGGGCGTGCGCATTGCGGCACGCTTG
AAGGTGATGTCATCTATGGATATGGCCGAACGGCGCAAGCCGCAGGATGGCCGTATCAAGATGAAGATTTCACGCAACAA
GTCCATCGACTTCCGGGTTAACACCCTGCCCACGCTCTGGGGCGAGAAGGTGGTTCTGCGTATTCTTGACGCCGAAAGCG
CCAAGATGGGTATTGATGCGCTGGGGTATGAAGAAGACCAGAAGGAAATGTACCTCACCGCGCTGGCCAGACCCCAGGGC
ATGATTCTGGTAACGGGCCCGACCGGCTCGGGCAAGACGGTATCGCTGTACACCGGCCTGAATATTCTCAACACCATGGA
GCGCAACATCTCCACCGCGGAAGATCCTGTCGAGATCAACCTTGAGGGTATCAACCAGGTCAACGTGAACCCCAAGCAGG
GGCTGGATTTTGCCAAGGCGCTGCGCGCGTTTTTGCGTCAGGATCCGGACATCATCATGGTCGGGGAGATCCGTGACCTT
GAAACGGCCGAAATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTACTGTCCACGCTACACACCAACAGCGCAGCTGA
GACTTTGACGCGTTTGCGCAATATGGGCGTGCCCTCGTTCAACATCGCCACATCCGTTAATCTGATCATCGCCCAGCGTC
TGGCTCGGCGGCTGTGCAAGTCATGCAAGGCGCCCTTTGAAGTGCCTCGCGAGACGCTCATTGAGGAAGGCTTCAGCCCT
GAAAAAATTGATGCAGGGTTGACTATTTACGGGCCGGTGGGTTGCGAAAACTGTAAAGACGGTTATAAAGGGCGTGTTGG
TATTTATGAGGTAGTTAAGATTACGCCAGCCATGCAACGTATCATCATGGAAGACGGCAACTCTATTCAGATTTCAGACG
TAGCCCAACAGGAAGGTTTCAGAAGCTTGCGCCAGTCGGCCCTGATGAAAGCAGAACAGGGCGTTACCAGTCTGGCGGAA
GTTAACCGAGTGACAAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2V3N2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.596

100

0.589
  pilB Acinetobacter baumannii D1279779

58.348

100

0.586
  pilB Legionella pneumophila strain ERS1305867

56.714

99.824

0.566
  pilB Vibrio cholerae strain A1552

50.98

98.942

0.504
  pilF Neisseria gonorrhoeae MS11

48.858

100

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

49.466

99.118

0.49
  pilB Vibrio campbellii strain DS40M4

48.404

99.471

0.481
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.755

93.474

0.381
  pilF Thermus thermophilus HB27

39.015

93.122

0.363