Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HG264_RS09165 Genome accession   NZ_CP051625
Coordinates   1995992..1996486 (-) Length   164 a.a.
NCBI ID   WP_169407373.1    Uniprot ID   A0A7Z2V3F6
Organism   Pseudomonas sp. gcc21     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1990992..2001486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HG264_RS09135 (HG264_09130) - 1991143..1991562 (+) 420 WP_169407368.1 DUF86 domain-containing protein -
  HG264_RS09140 (HG264_09135) - 1991774..1992145 (+) 372 WP_169407369.1 transposase -
  HG264_RS09145 (HG264_09140) - 1992254..1993375 (-) 1122 WP_169407370.1 Fic family protein -
  HG264_RS09150 (HG264_09145) - 1993710..1994186 (+) 477 WP_256663651.1 transposase -
  HG264_RS09155 (HG264_09150) - 1994700..1995098 (+) 399 WP_169407371.1 GFA family protein -
  HG264_RS09160 (HG264_09155) - 1995289..1995837 (+) 549 WP_169407372.1 transposase -
  HG264_RS09165 (HG264_09160) pilA 1995992..1996486 (-) 495 WP_169407373.1 pilin Machinery gene
  HG264_RS09170 (HG264_09165) pilB 1996850..1998553 (+) 1704 WP_169407374.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HG264_RS09175 (HG264_09170) pilC 1998557..1999822 (+) 1266 WP_169407375.1 type II secretion system F family protein Machinery gene
  HG264_RS09180 (HG264_09175) pilD 1999826..2000698 (+) 873 WP_169407376.1 A24 family peptidase Machinery gene
  HG264_RS09185 (HG264_09180) coaE 2000700..2001305 (+) 606 WP_169407377.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 164 a.a.        Molecular weight: 16647.98 Da        Isoelectric Point: 4.6920

>NTDB_id=439937 HG264_RS09165 WP_169407373.1 1995992..1996486(-) (pilA) [Pseudomonas sp. gcc21]
MKAQMQKGFTLIELMIVVAIIGILAAIALPAYQDYTKRAKVTEGLSLAASAKVAVAENAANGVAYDSGWTKPAATDNVTD
MTIGDNGEITISYGASIAATGGNTLVLYPFTGGTTTASLPADTAIYTPPADAIQWQCLASGATAKVSGATTGTLDAKLAP
SNCR

Nucleotide


Download         Length: 495 bp        

>NTDB_id=439937 HG264_RS09165 WP_169407373.1 1995992..1996486(-) (pilA) [Pseudomonas sp. gcc21]
ATGAAAGCTCAAATGCAGAAAGGTTTTACACTTATCGAACTGATGATCGTGGTTGCGATTATTGGTATTTTGGCGGCTAT
TGCTTTGCCGGCTTATCAGGATTATACAAAGCGGGCGAAAGTAACCGAGGGTCTTAGTTTGGCCGCTTCAGCGAAAGTGG
CTGTGGCAGAAAATGCTGCAAACGGTGTGGCTTATGACAGCGGTTGGACGAAACCTGCAGCGACAGACAACGTCACAGAT
ATGACGATTGGTGACAATGGCGAAATTACTATCAGTTATGGCGCATCCATTGCTGCTACAGGTGGCAACACCCTCGTTCT
TTATCCGTTCACAGGCGGTACTACTACAGCTTCGCTGCCAGCTGACACTGCGATTTATACCCCGCCTGCAGATGCGATCC
AGTGGCAGTGCTTGGCAAGTGGTGCTACGGCAAAAGTTTCAGGAGCGACAACCGGTACTCTAGATGCGAAACTGGCTCCT
TCCAACTGTCGGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)

Pilin (bacterial filament) (Pilin)

(38-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2V3F6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

49.419

100

0.518
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.089

100

0.506
  pilA2 Legionella pneumophila str. Paris

50

100

0.5
  pilA2 Legionella pneumophila strain ERS1305867

49.39

100

0.494
  comP Acinetobacter baylyi ADP1

41.875

97.561

0.409
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.372

100

0.402