Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   HF906_RS03565 Genome accession   NZ_CP051175
Coordinates   774700..775965 (-) Length   421 a.a.
NCBI ID   WP_003271622.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain Rs5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 769700..780965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF906_RS03535 (HF906_03540) - 770766..771650 (+) 885 WP_119447185.1 ATP-binding protein -
  HF906_RS03540 (HF906_03545) - 771628..772089 (+) 462 WP_043892400.1 NUDIX domain-containing protein -
  HF906_RS03545 (HF906_03550) yacG 772082..772297 (-) 216 WP_003271616.1 DNA gyrase inhibitor YacG -
  HF906_RS03550 (HF906_03555) zapD 772313..773071 (-) 759 WP_003271617.1 cell division protein ZapD -
  HF906_RS03555 (HF906_03560) coaE 773189..773803 (-) 615 WP_003271619.1 dephospho-CoA kinase -
  HF906_RS03560 (HF906_03565) pilD 773816..774697 (-) 882 WP_043892401.1 prepilin peptidase Machinery gene
  HF906_RS03565 (HF906_03570) pilG 774700..775965 (-) 1266 WP_003271622.1 type II secretion system F family protein Machinery gene
  HF906_RS03570 (HF906_03575) pilF 776010..777737 (-) 1728 WP_003271624.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HF906_RS03575 (HF906_03580) - 777985..779262 (-) 1278 WP_003271625.1 HlyC/CorC family transporter -
  HF906_RS03585 (HF906_03590) ispB 779537..780505 (-) 969 WP_003271627.1 octaprenyl diphosphate synthase -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46427.56 Da        Isoelectric Point: 10.3648

>NTDB_id=436594 HF906_RS03565 WP_003271622.1 774700..775965(-) (pilG) [Ralstonia solanacearum strain Rs5]
MATRAPTANRSAAASARKTASTKAPTQYIFEWEGKDRKGKVFKGEMRAESITEVNAVLRKQGLSITKSKRRRAARGKKIT
PKDVAYFTRQLSTMLKAGVPLLQSVDIIARGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPKYFDSLYCNLIDAGEQGG
ILDALLERLSMYMEKSIALKAQIKSAMIYPVSVLTVAFLVTVVLMIFVVPAFKNVFASFGATLPAPTLVVMGISDFFVKW
WYIIVFAPVVGIFLYARAFKRSENVQRNTHKAILKMPIFGSIIRKATIARWTRTLATMFSAGTPLVESMESVAGAAGNWV
YHDATLEIQQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVANISALIEPIIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=436594 HF906_RS03565 WP_003271622.1 774700..775965(-) (pilG) [Ralstonia solanacearum strain Rs5]
ATGGCCACACGAGCACCGACCGCGAACCGGTCCGCCGCCGCATCGGCACGCAAAACTGCCTCCACGAAGGCGCCCACGCA
GTACATCTTCGAGTGGGAAGGCAAGGACCGCAAAGGCAAAGTCTTCAAAGGCGAGATGCGGGCGGAGAGCATCACCGAAG
TCAACGCGGTGCTGCGCAAGCAGGGGCTGTCGATCACCAAGTCCAAGCGTCGGCGCGCGGCGCGCGGCAAGAAAATCACG
CCCAAGGACGTGGCCTACTTCACGCGCCAGTTGTCGACCATGCTGAAGGCCGGGGTGCCGCTGCTGCAATCGGTCGACAT
CATCGCGCGCGGCCACGCCAACCCGAACTTCACCCAGTTGCTGACGGAAATCCGCGTCGACATCGAGTCGGGCAGCAGCA
TGGCGCAGGCGTTCCGCCGCCATCCGAAGTACTTCGATTCGCTGTACTGCAACCTGATCGACGCGGGCGAACAGGGCGGT
ATTCTCGACGCGCTGCTCGAGCGGCTGTCGATGTACATGGAAAAATCGATCGCGCTGAAGGCGCAGATCAAGTCGGCCAT
GATCTACCCGGTCTCGGTGCTGACGGTGGCCTTCCTGGTCACGGTGGTGCTGATGATCTTCGTGGTGCCCGCTTTCAAGA
ACGTGTTCGCCAGCTTCGGCGCCACGCTGCCAGCACCGACGCTGGTGGTGATGGGCATCTCGGACTTCTTCGTGAAGTGG
TGGTACATCATCGTCTTCGCCCCCGTTGTCGGCATCTTCCTGTATGCGCGGGCCTTCAAGCGCTCGGAGAACGTGCAGCG
CAACACGCACAAGGCCATCCTGAAGATGCCGATCTTCGGCAGCATCATCCGCAAGGCCACCATCGCGCGCTGGACGCGCA
CGCTGGCGACGATGTTCTCCGCCGGTACGCCGCTGGTGGAATCGATGGAGTCGGTGGCCGGTGCGGCCGGCAACTGGGTC
TACCACGACGCCACCCTCGAGATCCAGCAGGCGGTACGCATCGGTACCAGCCTCACCAACGCCATGCAGGCCACGCACGT
GTTCGACAACATGGTGCTGCAGATGACGCAGATCGGCGAGGAATCCGGTGCGCTGGACAACATGCTGCTGAAAGTGGCCG
AGTTCTACGAGCGCGAGGTCGATGACGCGGTGGCCAACATCTCCGCCCTGATCGAGCCGATCATCATCGTGATCCTCGGC
GTGCTGATCGGCGGCATGGTGGTCGCCATGTACCTGCCGATCTTCAAGCTGGGCCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

51.128

94.774

0.485
  pilG Neisseria meningitidis 44/76-A

50.877

94.774

0.482
  pilC Pseudomonas stutzeri DSM 10701

49.751

95.487

0.475
  pilC Acinetobacter baumannii D1279779

46.683

96.675

0.451
  pilC Legionella pneumophila strain ERS1305867

46.535

95.962

0.447
  pilC Acinetobacter baylyi ADP1

45.946

96.675

0.444
  pilC Vibrio campbellii strain DS40M4

38.765

96.2

0.373
  pilC Vibrio cholerae strain A1552

39

95.012

0.371
  pilC Thermus thermophilus HB27

37.811

95.487

0.361