Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HF906_RS03570 Genome accession   NZ_CP051175
Coordinates   776010..777737 (-) Length   575 a.a.
NCBI ID   WP_003271624.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain Rs5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 771010..782737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF906_RS03540 (HF906_03545) - 771628..772089 (+) 462 WP_043892400.1 NUDIX domain-containing protein -
  HF906_RS03545 (HF906_03550) yacG 772082..772297 (-) 216 WP_003271616.1 DNA gyrase inhibitor YacG -
  HF906_RS03550 (HF906_03555) zapD 772313..773071 (-) 759 WP_003271617.1 cell division protein ZapD -
  HF906_RS03555 (HF906_03560) coaE 773189..773803 (-) 615 WP_003271619.1 dephospho-CoA kinase -
  HF906_RS03560 (HF906_03565) pilD 773816..774697 (-) 882 WP_043892401.1 prepilin peptidase Machinery gene
  HF906_RS03565 (HF906_03570) pilG 774700..775965 (-) 1266 WP_003271622.1 type II secretion system F family protein Machinery gene
  HF906_RS03570 (HF906_03575) pilF 776010..777737 (-) 1728 WP_003271624.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HF906_RS03575 (HF906_03580) - 777985..779262 (-) 1278 WP_003271625.1 HlyC/CorC family transporter -
  HF906_RS03585 (HF906_03590) ispB 779537..780505 (-) 969 WP_003271627.1 octaprenyl diphosphate synthase -
  HF906_RS03590 (HF906_03595) rplU 780783..781094 (+) 312 WP_003271629.1 50S ribosomal protein L21 -
  HF906_RS03595 (HF906_03600) rpmA 781132..781392 (+) 261 WP_003271630.1 50S ribosomal protein L27 -
  HF906_RS03600 (HF906_03605) obgE 781591..782691 (+) 1101 WP_003271633.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63812.58 Da        Isoelectric Point: 6.9141

>NTDB_id=436595 HF906_RS03570 WP_003271624.1 776010..777737(-) (pilF) [Ralstonia solanacearum strain Rs5]
MTLGLALTQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPAVLSTNKHF
QQLRLLPLGRRDNRVIVATSDPSDPKPIEELRQKMNVAIETVIVEHDKLVRQLSLANEAPPEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKETVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEEDLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=436595 HF906_RS03570 WP_003271624.1 776010..777737(-) (pilF) [Ralstonia solanacearum strain Rs5]
ATGACTCTCGGACTCGCACTTACGCAGAGCCGGCGAATTGCGCCGACATTACTCACTCAGCTAGAGCAGAGCGCGCGCGA
AAAGCGCACGCAGTTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCGCACGACCTCGCCGTCTTCGTGGCGGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGACACCGTGCCGGCCGTGCTGTCGACCAACAAGCATTTC
CAGCAATTGCGCCTGCTGCCGCTGGGCCGGCGCGACAACCGCGTCATCGTCGCCACCTCGGACCCGAGCGACCCGAAGCC
GATCGAAGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTCCGCCAGCTGA
GCCTCGCCAACGAAGCGCCGCCCGAGATCAAGTCGCTCATCCCAAAGCAGGAAACCACCCAGATCGAATACGACCCGGGC
GCGGCCGCAGCGGTGCGGCGTACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCACTGCTGCGCGGCACGTCCGACTTGCACTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTTGAACAAGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGTATCAAGGTGTTGTCGCGCCTG
GACATCTCGGAAAAACGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGACGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATAGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAACGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAAACGGTGTCGCTGTACACCTTCCT
GAACAGGCTGAACCAGGGCGACATCAACATTTCCACGGCGGAAGATCCGGCGGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGCCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGATATCTCCATCAAGGCCGCACAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTCACACGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCTCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGCCCACTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAAGAAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACCGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAGGGCGTGCTCTCGCTGCGCGACTCCGGCCTGAGAAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGCTGGCCACGACGAACCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.945

94.783

0.511
  pilB Acinetobacter baumannii D1279779

51.15

98.261

0.503
  pilB Acinetobacter baylyi ADP1

49.912

98.261

0.49
  pilB Legionella pneumophila strain ERS1305867

46.235

99.304

0.459
  pilB Vibrio cholerae strain A1552

44.643

97.391

0.435
  pilB Vibrio campbellii strain DS40M4

44.708

95.304

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

45.018

94.261

0.424
  pilB/pilB1 Synechocystis sp. PCC 6803

36.589

100

0.384
  pilF Thermus thermophilus HB27

37.5

97.391

0.365