Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HB763_RS03265 Genome accession   NZ_CP050475
Coordinates   700712..702460 (-) Length   582 a.a.
NCBI ID   WP_255985749.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC012     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 695712..707460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB763_RS03245 (HB763_03255) - 696417..697256 (-) 840 WP_255985376.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  HB763_RS03250 (HB763_03260) - 697326..698819 (-) 1494 WP_255985377.1 SPOR domain-containing protein -
  HB763_RS03255 (HB763_03265) aroB 698873..699973 (-) 1101 WP_255985378.1 3-dehydroquinate synthase -
  HB763_RS03260 (HB763_03270) aroK 700005..700523 (-) 519 WP_010443904.1 shikimate kinase AroK -
  HB763_RS03265 (HB763_03275) pilQ 700712..702460 (-) 1749 WP_255985749.1 type IV pilus secretin PilQ Machinery gene
  HB763_RS03270 (HB763_03280) pilP 702495..703010 (-) 516 WP_255985379.1 pilus assembly protein PilP Machinery gene
  HB763_RS03275 (HB763_03285) pilO 703000..703593 (-) 594 WP_255985380.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB763_RS03280 (HB763_03290) pilN 703586..704161 (-) 576 WP_255985381.1 PilN domain-containing protein Machinery gene
  HB763_RS03285 (HB763_03295) pilM 704145..705161 (-) 1017 WP_255985382.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63717.60 Da        Isoelectric Point: 4.5130

>NTDB_id=433150 HB763_RS03265 WP_255985749.1 700712..702460(-) (pilQ) [Vibrio campbellii strain LJC012]
MKMRHGLTLTPRKLTGLFSLWFSLLLASFVAAEEVQSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYSHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEKEIGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGDGEGDDGGSSVGIDDFLNVNLAATSPNA
TSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=433150 HB763_RS03265 WP_255985749.1 700712..702460(-) (pilQ) [Vibrio campbellii strain LJC012]
ATGAAAATGAGACACGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTCTCTCTACTTTTAGC
CTCTTTCGTCGCAGCAGAAGAAGTACAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACGAACAAAGACAAAGACGCCG
TGATTGTGGTTGAGCTCGCATCACCCGCTGCAATTGTTGATATTAAACGCGTACAAGAAGGCTTGAGTATCGACTTACTC
AACACCTCCGTTAAAGATGACCAGCTTTACCTACTTGATGTGAAAGATTTCTCGACGGTTGTCGAGAGCGTTGAGGTATT
CCGCGATACTTCTACAACACGTCTGGTGGCTTATATAGATGAGGACTATTCTCACGACTACCGTCTTACTGGTCGTTATT
TAGAGGTAAAGGTTGGCAAGCTTAAAGCCAACGAAAAAGCGCCGGATAAAAGCATTTTGGAAAAAGAAGGCAAGCTAATT
TCGATTAACTTCCAAGACATTCCTGTGCGAAACGTGTTGCAACTTATCGCCGACTACAATCAATTTAACTTGGTCGTCTC
TGACTCGGTCGAGGGTAACTTGACCTTGCGTTTGGATGGTGTGCCTTGGCAGCAAGTACTCGACATTATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGTAATGTCATTCTCGTCGCACCAAAAGATGAATTGGATCTGCGCGAGAAGCAGCAA
CTCGAAAAGCAAAAGCTAGAGAAAGAGATCGGTGAGCTTTCATCAGAGATCATCAAGGTGAACTTCGCTAAAGCGTCAGA
CATTGCTGAGATGATCAATGGAGAGGGCAACATCAGTATGCTTTCAGATCGAGGCAGCATGACCATTGATGAGCGTACCA
ACTCGTTATTGATTCGTGAGTTACCTGAGAATATTGAAGTGATTCGCGAGATCATAGAATCATTGGATATTCCTGTAAAA
CAGGTTCAGATTGAAGCGCGTATTGTGACTATTAATGAAGGTAACATGGACGAACTTGGCGTTCGTTGGGGCTTCACCTC
CATTAATGGCAGTCATACTGTGGGTGGTTCAATTGAGAATAACCTTGCGACAATCGGTTTGTACGATGGCGGCGGTGACG
GTGAAGGTGATGACGGTGGCAGCAGTGTGGGTATTGACGACTTTCTCAACGTTAACCTTGCTGCGACCAGTCCAAATGCG
ACCAGTATTGCCTTCCAAGTTGCGAAGTTAGGTTCGGATACCTTACTAGATTTGGAACTTTCGGCGCTGCAACAAGAATC
GAAAGCAGAAATTATTTCTAGCCCACGTTTGATAACCACGAATAAGAAGCCAGCTTACATTGAGCAGGGTACCGAGATCC
CATACCTTGAATCTTCTTCCAGTGGTGCGACTTCTGTGACGTTCAAAAAAGCGGTATTGAGCTTGAAAGTGACGCCACAG
ATTACGCCTGACAACCGTTTGGTGTTGGATTTAAGCGTTACGCAAGACAGACCGGGCCAAGTGGTAAAAACAGGCACTGG
TGAGGCGGTGGCGATAGATACGCAACGTATTGGTACACAAGTTTTAGTTAATAATGGCGAGACAGTCGTGTTGGGCGGTA
TTTTTCAACACAGCATTACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTGCCGCTGTTAGGTGCGCTTTTCCGT
CGAAGTTACGAAAATGTCGGTAAAAGTGAACTACTGATTTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(422-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

99.141

100

0.991
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.465

97.595

0.756
  pilQ Vibrio cholerae strain A1552

77.465

97.595

0.756
  pilQ Legionella pneumophila strain ERS1305867

41.742

94.674

0.395
  pilQ Legionella pneumophila strain Lp02

41.742

94.674

0.395
  pilQ Pseudomonas aeruginosa PAK

40.58

94.845

0.385
  pilQ Acinetobacter baumannii D1279779

37.268

100

0.38
  pilQ Acinetobacter baumannii strain A118

37.268

100

0.38
  comQ Acinetobacter baylyi ADP1

37.743

97.423

0.368