Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HB763_RS03275 Genome accession   NZ_CP050475
Coordinates   703000..703593 (-) Length   197 a.a.
NCBI ID   WP_255985380.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC012     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 698000..708593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB763_RS03255 (HB763_03265) aroB 698873..699973 (-) 1101 WP_255985378.1 3-dehydroquinate synthase -
  HB763_RS03260 (HB763_03270) aroK 700005..700523 (-) 519 WP_010443904.1 shikimate kinase AroK -
  HB763_RS03265 (HB763_03275) pilQ 700712..702460 (-) 1749 WP_255985749.1 type IV pilus secretin PilQ Machinery gene
  HB763_RS03270 (HB763_03280) pilP 702495..703010 (-) 516 WP_255985379.1 pilus assembly protein PilP Machinery gene
  HB763_RS03275 (HB763_03285) pilO 703000..703593 (-) 594 WP_255985380.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB763_RS03280 (HB763_03290) pilN 703586..704161 (-) 576 WP_255985381.1 PilN domain-containing protein Machinery gene
  HB763_RS03285 (HB763_03295) pilM 704145..705161 (-) 1017 WP_255985382.1 type IV pilus assembly protein PilM Machinery gene
  HB763_RS03290 (HB763_03300) - 705340..707907 (+) 2568 WP_255985383.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23004.55 Da        Isoelectric Point: 4.9505

>NTDB_id=433152 HB763_RS03275 WP_255985380.1 703000..703593(-) (pilO) [Vibrio campbellii strain LJC012]
MVNWQDLEMDEIAEWPLVPQLVAILLMVVLIQGFGYWFYLKPEEARLNTLIQEEQTLKVALRIKANKVAAVPQLQSQLDE
LTTRYELLLKQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKESEHFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFQDVTWQRVSQESETLHFRVRANTYQFKLEEKKDAK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=433152 HB763_RS03275 WP_255985380.1 703000..703593(-) (pilO) [Vibrio campbellii strain LJC012]
ATGGTTAATTGGCAAGACCTAGAAATGGATGAAATCGCTGAATGGCCGCTGGTACCTCAGTTGGTGGCGATTCTTCTTAT
GGTGGTGTTGATCCAAGGTTTTGGTTACTGGTTTTATTTGAAGCCAGAGGAAGCTCGCCTTAATACCTTGATCCAAGAAG
AGCAGACTTTAAAAGTCGCGCTGAGGATCAAAGCCAATAAAGTCGCTGCAGTGCCGCAGCTTCAGTCACAACTTGATGAG
CTGACCACCCGCTATGAACTGCTTTTGAAACAACTGCCTGCACAGAAAGAATTGGCGACCATGCTAGCGTCGGTTAATGA
ACTCGGCATCGATAACAAGCTGACATTTACACGTATTGATTGGGGAGATAAAGAGTCAGAGCACTTCCTTTACCGTTTGC
CGCTCAATATTGAGCTAACGGGCGACTTCCATGACATCGGTCGTTTTTCACAGGCCATCGCCACCTTACCACGCATCATC
ACATTTCAAGATGTAACTTGGCAGCGAGTCAGTCAAGAGAGTGAAACCTTGCACTTCCGTGTAAGAGCAAACACTTACCA
ATTCAAACTAGAGGAAAAGAAAGATGCGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

98.413

95.939

0.944
  pilO Vibrio cholerae strain A1552

71.728

96.954

0.695