Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HB763_RS03285 Genome accession   NZ_CP050475
Coordinates   704145..705161 (-) Length   338 a.a.
NCBI ID   WP_255985382.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC012     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 699145..710161
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB763_RS03260 (HB763_03270) aroK 700005..700523 (-) 519 WP_010443904.1 shikimate kinase AroK -
  HB763_RS03265 (HB763_03275) pilQ 700712..702460 (-) 1749 WP_255985749.1 type IV pilus secretin PilQ Machinery gene
  HB763_RS03270 (HB763_03280) pilP 702495..703010 (-) 516 WP_255985379.1 pilus assembly protein PilP Machinery gene
  HB763_RS03275 (HB763_03285) pilO 703000..703593 (-) 594 WP_255985380.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB763_RS03280 (HB763_03290) pilN 703586..704161 (-) 576 WP_255985381.1 PilN domain-containing protein Machinery gene
  HB763_RS03285 (HB763_03295) pilM 704145..705161 (-) 1017 WP_255985382.1 type IV pilus assembly protein PilM Machinery gene
  HB763_RS03290 (HB763_03300) - 705340..707907 (+) 2568 WP_255985383.1 penicillin-binding protein 1A -
  HB763_RS03295 (HB763_03305) oxyR 707986..708894 (-) 909 WP_255985384.1 DNA-binding transcriptional regulator OxyR -
  HB763_RS03300 (HB763_03310) - 709069..709797 (+) 729 WP_005432732.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37940.29 Da        Isoelectric Point: 6.1128

>NTDB_id=433154 HB763_RS03285 WP_255985382.1 704145..705161(-) (pilM) [Vibrio campbellii strain LJC012]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIFAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKNLQIEQNLEESEKEFAVVQTFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGSRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGATTPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=433154 HB763_RS03285 WP_255985382.1 704145..705161(-) (pilM) [Vibrio campbellii strain LJC012]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTTTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGTGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCT
GTGATCAGTAAAAATCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAACCTTCTCACA
TCAATCTCCATTCCCCGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTAGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACGATGTTCACCGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GCGGTTCTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTAGCAGACGATGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAGGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTACAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTCTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

97.337

100

0.973
  pilM Vibrio cholerae strain A1552

49.415

100

0.5