Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HB762_RS14830 Genome accession   NZ_CP050470
Coordinates   3128058..3129812 (+) Length   584 a.a.
NCBI ID   WP_255901950.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC013     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3123058..3134812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB762_RS14810 (HB762_14715) pilM 3125357..3126373 (+) 1017 WP_255899045.1 type IV pilus biogenesis protein PilM Machinery gene
  HB762_RS14815 (HB762_14720) pilN 3126357..3126932 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  HB762_RS14820 (HB762_14725) pilO 3126925..3127518 (+) 594 WP_255899047.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB762_RS14825 (HB762_14730) pilP 3127508..3128023 (+) 516 WP_255899049.1 pilus assembly protein PilP Machinery gene
  HB762_RS14830 (HB762_14735) pilQ 3128058..3129812 (+) 1755 WP_255901950.1 type IV pilus secretin PilQ Machinery gene
  HB762_RS14835 (HB762_14740) aroK 3130001..3130519 (+) 519 WP_010443904.1 shikimate kinase AroK -
  HB762_RS14840 (HB762_14745) aroB 3130551..3131651 (+) 1101 WP_255899052.1 3-dehydroquinate synthase -
  HB762_RS14845 (HB762_14750) - 3131705..3133198 (+) 1494 WP_255899055.1 SPOR domain-containing protein -
  HB762_RS14850 (HB762_14755) - 3133268..3134107 (+) 840 WP_038889578.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 584 a.a.        Molecular weight: 63942.80 Da        Isoelectric Point: 4.4499

>NTDB_id=433114 HB762_RS14830 WP_255901950.1 3128058..3129812(+) (pilQ) [Vibrio campbellii strain LJC013]
MKMRHGLTLTPRKLTGLFSLWFSLLLAPFVAAEEVQSNSLESIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYSHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGLPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEKEIGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGEGEGEGDDGGSSVGIDDFLNVNLAATSP
NATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVT
PQITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGAL
FRRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1755 bp        

>NTDB_id=433114 HB762_RS14830 WP_255901950.1 3128058..3129812(+) (pilQ) [Vibrio campbellii strain LJC013]
ATGAAAATGAGACACGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTCTCTCTACTTTTAGC
CCCTTTCGTCGCAGCAGAAGAAGTACAGTCTAACTCGCTTGAGAGCATCGACTTCCGTACGAACAAAGACAAAGACGCCG
TGATTGTGGTTGAGCTCGCATCACCCGCTGCGATTGTTGATATTAAACGCGTACAAGAAGGCTTGAGTATCGACTTACTC
AACACCTCCGTTAAAGATGACCAGCTTTACCTACTTGATGTGAAAGATTTCTCGACGGTTGTCGAGAGCGTTGAGGTATT
CCGCGATACTTCTACAACACGTCTGGTGGCTTATATCGATGAGGACTATTCTCACGACTACCGTCTTACTGGTCGTTATT
TAGAGGTAAAGGTTGGCAAGCTTAAAGCCAACGAAAAAGCGCCGGATAAAAGCATTTTGGAAAAAGAAGGCAAGCTAATT
TCGATTAACTTCCAAGACATTCCTGTGCGAAACGTGTTGCAACTTATCGCCGACTACAATCAATTTAACTTGGTCGTCTC
TGACTCGGTCGAGGGTAACTTGACCTTGCGTTTGGATGGTTTGCCTTGGCAGCAAGTACTCGACATTATCCTGCAAGTAA
AAGGGTTAGATAAGCGTGTTGATGGCAATGTCATTCTCGTCGCACCAAAAGATGAATTGGATCTGCGCGAGAAGCAGCAA
CTCGAAAAGCAAAAGCTAGAGAAAGAGATCGGTGAGCTTTCATCAGAGATCATCAAGGTGAACTTCGCTAAAGCGTCAGA
CATTGCTGAGATGATCAATGGAGAGGGCAACATCAGTATGCTTTCAGATCGAGGCAGCATGACCATTGATGAGCGTACCA
ACTCGTTATTGATTCGTGAGTTACCTGAGAATATTGAAGTGATTCGCGAGATCATAGAATCATTGGATATTCCTGTAAAA
CAGGTTCAGATTGAAGCGCGTATTGTGACTATTAATGAAGGTAACATGGACGAACTTGGCGTTCGTTGGGGCTTCACCTC
CATTAATGGCAGTCATACTGTGGGTGGTTCAATTGAGAATAACCTTGCGACAATCGGCTTGTACGATGGCGGCGGTGAAG
GTGAAGGTGAAGGTGATGACGGTGGCAGCAGTGTAGGTATTGACGACTTTCTCAACGTTAACCTTGCTGCGACCAGTCCA
AATGCGACCAGTATTGCCTTCCAAGTTGCGAAGTTAGGTTCGGATACCTTACTAGATTTGGAACTTTCGGCGCTGCAACA
AGAATCGAAAGCAGAAATTATTTCCAGTCCACGTTTGATCACCACGAATAAGAAGCCAGCTTACATTGAGCAGGGTACCG
AGATCCCTTACCTTGAATCTTCTTCCAGTGGTGCGACTTCTGTGACGTTCAAAAAAGCGGTATTGAGCTTGAAAGTGACG
CCACAGATTACGCCTGACAACCGTTTGGTGTTGGATTTGAGCGTTACGCAAGACAGACCAGGCCAAGTGGTAAAAACAGG
CACTGGTGAGGCGGTGGCGATCGATACGCAACGTATTGGTACACAAGTTTTAGTCAATAATGGCGAGACAGTCGTATTGG
GCGGTATTTTTCAACACAGCATTACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTGCCGCTGTTAGGTGCGCTT
TTCCGTCGAAGTTACGAAAATGTCGGTAAAAGTGAACTACTGATTTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(424-582)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

98.63

100

0.986
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.193

97.603

0.753
  pilQ Vibrio cholerae strain A1552

77.193

97.603

0.753
  pilQ Legionella pneumophila strain ERS1305867

40.82

96.062

0.392
  pilQ Legionella pneumophila strain Lp02

41.198

94.349

0.389
  pilQ Pseudomonas aeruginosa PAK

39.859

97.089

0.387
  pilQ Acinetobacter baumannii strain A118

37.105

100

0.382
  pilQ Acinetobacter baumannii D1279779

37.105

100

0.382
  comQ Acinetobacter baylyi ADP1

36.707

99.829

0.366