Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HB762_RS14820 Genome accession   NZ_CP050470
Coordinates   3126925..3127518 (+) Length   197 a.a.
NCBI ID   WP_255899047.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC013     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3121925..3132518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB762_RS14805 (HB762_14710) - 3122611..3125178 (-) 2568 WP_255899043.1 penicillin-binding protein 1A -
  HB762_RS14810 (HB762_14715) pilM 3125357..3126373 (+) 1017 WP_255899045.1 type IV pilus biogenesis protein PilM Machinery gene
  HB762_RS14815 (HB762_14720) pilN 3126357..3126932 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  HB762_RS14820 (HB762_14725) pilO 3126925..3127518 (+) 594 WP_255899047.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB762_RS14825 (HB762_14730) pilP 3127508..3128023 (+) 516 WP_255899049.1 pilus assembly protein PilP Machinery gene
  HB762_RS14830 (HB762_14735) pilQ 3128058..3129812 (+) 1755 WP_255901950.1 type IV pilus secretin PilQ Machinery gene
  HB762_RS14835 (HB762_14740) aroK 3130001..3130519 (+) 519 WP_010443904.1 shikimate kinase AroK -
  HB762_RS14840 (HB762_14745) aroB 3130551..3131651 (+) 1101 WP_255899052.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23011.51 Da        Isoelectric Point: 4.9464

>NTDB_id=433112 HB762_RS14820 WP_255899047.1 3126925..3127518(+) (pilO) [Vibrio campbellii strain LJC013]
MVNWQDLEMDEIAEWPLVPQLVAILLMVVLIQGFGYWFYLKPEEARLNTLIHEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKESEHFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFQDVTWQRVSQESETLHFRVRANTYQFKPEEKKDAK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=433112 HB762_RS14820 WP_255899047.1 3126925..3127518(+) (pilO) [Vibrio campbellii strain LJC013]
ATGGTTAATTGGCAAGACCTAGAAATGGATGAAATCGCTGAATGGCCGCTGGTACCTCAGTTGGTGGCGATTCTTCTTAT
GGTGGTGTTGATCCAAGGTTTTGGTTACTGGTTTTATTTGAAGCCAGAGGAAGCTCGCCTTAATACCTTGATCCATGAAG
AGCAGACTTTAAAAGTCGCGCTGAGGATCAAAGCCAATAAAGTAGCTGCACTGCCGCAGCTTCAGTCACAACTTGATGAA
CTGACCACCCGCTATGAACTGCTTTTGCAACAACTGCCGGCACAGAAAGAATTGGCGACCATGCTAGCGTCGGTTAATGA
ACTCGGCATCGATAACAAGCTGACATTTACGCGTATTGATTGGGGAGATAAAGAGTCAGAGCACTTCCTTTACCGTTTGC
CGCTCAATATTGAGCTAACGGGTGACTTCCATGACATCGGTCGTTTTTCACAGGCCATCGCCACCTTACCACGCATCATC
ACATTTCAAGATGTAACTTGGCAGCGAGTCAGTCAAGAGAGTGAAACCTTGCACTTCCGTGTAAGAGCAAACACTTACCA
ATTCAAACCAGAGGAAAAGAAAGATGCGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

100

96.447

0.964
  pilO Vibrio cholerae strain A1552

71.728

96.954

0.695