Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HMPREF0389_RS01040 Genome accession   NC_016630
Coordinates   242401..243456 (+) Length   351 a.a.
NCBI ID   WP_041250917.1    Uniprot ID   -
Organism   Filifactor alocis ATCC 35896     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 237401..248456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMPREF0389_RS01025 (HMPREF0389_00428) - 238192..239076 (+) 885 WP_014261882.1 pyridoxamine kinase -
  HMPREF0389_RS01030 (HMPREF0389_00427) - 239635..240063 (+) 429 WP_014261883.1 hypothetical protein -
  HMPREF0389_RS01035 (HMPREF0389_00426) - 240449..242119 (+) 1671 WP_014261884.1 GspE/PulE family protein -
  HMPREF0389_RS01040 (HMPREF0389_00425) pilT 242401..243456 (+) 1056 WP_041250917.1 type IV pilus twitching motility protein PilT Machinery gene
  HMPREF0389_RS01045 (HMPREF0389_00424) - 243483..243695 (+) 213 WP_014261886.1 hypothetical protein -
  HMPREF0389_RS01050 (HMPREF0389_01681) - 243669..244751 (-) 1083 WP_049770186.1 IS30 family transposase -
  HMPREF0389_RS01055 (HMPREF0389_00421) - 244802..245887 (+) 1086 WP_014261887.1 type II secretion system F family protein -
  HMPREF0389_RS01060 (HMPREF0389_00420) - 245988..246356 (+) 369 WP_014261888.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HMPREF0389_RS01065 (HMPREF0389_00419) - 246920..247609 (+) 690 WP_041250736.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39168.12 Da        Isoelectric Point: 6.2460

>NTDB_id=43198 HMPREF0389_RS01040 WP_041250917.1 242401..243456(+) (pilT) [Filifactor alocis ATCC 35896]
MTLEPIIRESIRTGASDIHLSVGIPPVLRIHGKLEKVGNYSLTAEDTESLANELLNEEEMEFFEEEGEIDVAYTFLEVCR
YRVNVYREQGNFCIALRTITSAIPKMEDLKLPPVFKEFCKKKRGLILVTGPTGSGKSTTLASMVGYINQIRKEHIILIED
PIEYVHTHGNSVINQRELGRDTKSFANALRASLREDPDVILVGEMRDLETISTAITAAETGHLVMSTLHTIGAAKTIDRI
IDIFPPHQQLQVRSQLSSVLEAVISQQLLPIANNRGRVVGLEIMSSTPALRNLIREGKTHQIQNVIQTNYGIGMRTMDGS
LVDLFRKGIITKETLFEYAVDKESVKGLLSK

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=43198 HMPREF0389_RS01040 WP_041250917.1 242401..243456(+) (pilT) [Filifactor alocis ATCC 35896]
ATGACATTGGAACCTATTATCAGAGAAAGTATCCGAACAGGCGCTTCAGATATCCATCTTTCGGTCGGAATTCCTCCTGT
TTTAAGGATTCACGGGAAGTTGGAAAAGGTAGGAAATTACAGTCTGACAGCAGAAGATACCGAAAGTTTGGCAAACGAAC
TTCTCAATGAAGAAGAAATGGAATTTTTTGAAGAAGAAGGTGAAATTGACGTTGCCTACACTTTTTTGGAAGTTTGTCGT
TATCGGGTAAATGTTTACAGGGAACAGGGAAATTTCTGTATTGCACTTCGTACTATCACTTCTGCTATTCCTAAGATGGA
GGATTTGAAACTTCCACCTGTGTTTAAGGAATTTTGCAAAAAGAAAAGAGGATTAATTTTGGTAACAGGACCGACCGGAA
GCGGAAAATCGACCACTTTAGCATCCATGGTTGGATATATCAACCAAATTCGAAAAGAACATATTATCTTGATTGAAGAT
CCGATTGAGTATGTTCATACTCATGGAAACAGTGTCATCAATCAAAGAGAGCTCGGACGAGATACCAAGAGCTTCGCCAA
CGCACTTCGTGCATCACTTCGGGAAGATCCGGATGTTATTCTGGTAGGGGAAATGCGTGACTTAGAGACGATTTCGACAG
CGATTACCGCTGCCGAAACAGGTCACTTGGTAATGTCCACCTTGCATACCATTGGAGCAGCGAAAACTATCGACCGTATT
ATTGATATTTTCCCGCCACACCAACAGTTGCAAGTTCGTTCACAATTGTCTTCTGTGCTTGAAGCCGTTATCTCACAACA
ATTGCTGCCAATTGCAAACAACAGAGGTCGAGTGGTAGGGTTGGAAATTATGTCTTCCACTCCGGCGCTACGCAATTTGA
TTCGTGAAGGAAAAACACATCAAATTCAAAATGTTATCCAAACAAATTACGGAATCGGTATGAGAACGATGGATGGTTCG
TTAGTAGATTTGTTCAGAAAAGGAATTATCACAAAAGAAACACTTTTTGAATATGCAGTAGACAAAGAGTCCGTCAAAGG
CTTATTGTCAAAATAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(37-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

49.419

98.006

0.484
  pilT Acinetobacter baumannii D1279779

49.419

98.006

0.484
  pilT Acinetobacter baumannii strain A118

49.419

98.006

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.296

96.296

0.484
  pilT Acinetobacter baylyi ADP1

49.128

98.006

0.481
  pilT Legionella pneumophila strain Lp02

49.415

97.436

0.481
  pilT Legionella pneumophila strain ERS1305867

49.415

97.436

0.481
  pilT Neisseria meningitidis 8013

50

95.157

0.476
  pilT Neisseria gonorrhoeae MS11

49.701

95.157

0.473
  pilT Vibrio cholerae strain A1552

49.85

94.872

0.473
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.85

94.872

0.473
  pilT Pseudomonas stutzeri DSM 10701

47.965

98.006

0.47
  pilT Pseudomonas aeruginosa PAK

48.378

96.581

0.467
  pilU Pseudomonas stutzeri DSM 10701

40.413

96.581

0.39
  pilU Acinetobacter baylyi ADP1

39.264

92.877

0.365

Multiple sequence alignment