Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VFU_RS03125 Genome accession   NC_016602
Coordinates   656672..658396 (+) Length   574 a.a.
NCBI ID   WP_041942471.1    Uniprot ID   -
Organism   Vibrio furnissii NCTC 11218     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 651672..663396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VFU_RS03105 (vfu_A00629) pilM 653978..654982 (+) 1005 WP_014204334.1 type IV pilus assembly protein PilM Machinery gene
  VFU_RS03110 (vfu_A00630) pilN 654972..655562 (+) 591 WP_014204335.1 PilN domain-containing protein Machinery gene
  VFU_RS03115 (vfu_A00631) pilO 655555..656145 (+) 591 WP_014204336.1 type 4a pilus biogenesis protein PilO Machinery gene
  VFU_RS03120 (vfu_A00632) pilP 656135..656647 (+) 513 WP_041942470.1 pilus assembly protein PilP Machinery gene
  VFU_RS03125 (vfu_A00633) pilQ 656672..658396 (+) 1725 WP_041942471.1 type IV pilus secretin PilQ Machinery gene
  VFU_RS03130 (vfu_A00634) aroK 658584..659102 (+) 519 WP_004728569.1 shikimate kinase AroK -
  VFU_RS03135 (vfu_A00635) aroB 659130..660215 (+) 1086 WP_004728570.1 3-dehydroquinate synthase -
  VFU_RS03140 (vfu_A00636) - 660261..661781 (+) 1521 WP_014204339.1 SPOR domain-containing protein -
  VFU_RS03145 (vfu_A00637) - 661876..662706 (+) 831 WP_014204340.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 61962.64 Da        Isoelectric Point: 4.4610

>NTDB_id=42933 VFU_RS03125 WP_041942471.1 656672..658396(+) (pilQ) [Vibrio furnissii NCTC 11218]
MIKGLNASTAWGIRCLFVGVCLLLSAATFAQEAAANQLKNIDFRINKDKAAVLIVELASASAVVDVQKTEQGLTIELLKT
DVSDDKLYLVDVRDFATAVENIEVFRQQPSTVLVATVQGAFNYEYSLKGNYLEVVISKVSAQEPQPKSVLEKEGKLISIN
FQDIPVRNVLQLIADYNQFNLVVSDSVSGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDRREQQQLEK
ARMAEELGDLQSEIIKINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLLRELPDNIHVIRDIVASLDIPVKQVQ
IEARIVTVNEGDLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGSSDSGDGAAIDDFLNVNLAATSANASSIAFQVA
KLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLV
LDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSVTSSVDKVPLLGDLPLLGALFRRSYEQMGK
SELLIFVTPKVVIQ

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=42933 VFU_RS03125 WP_041942471.1 656672..658396(+) (pilQ) [Vibrio furnissii NCTC 11218]
ATGATTAAAGGACTAAATGCCTCCACGGCTTGGGGAATACGTTGTCTGTTCGTGGGCGTATGCCTGCTGTTATCTGCCGC
CACATTTGCCCAGGAAGCGGCGGCCAATCAGCTGAAAAATATCGACTTTCGCATCAATAAAGACAAAGCGGCGGTGTTGA
TTGTCGAACTGGCGTCGGCTTCTGCGGTGGTGGATGTGCAGAAAACGGAGCAAGGATTGACGATCGAATTGCTTAAAACC
GATGTTAGCGATGACAAACTGTATTTGGTGGACGTGCGCGATTTTGCCACGGCGGTGGAAAACATCGAAGTGTTTCGCCA
GCAACCGAGCACGGTGCTGGTGGCGACGGTGCAAGGGGCATTCAATTACGAATACAGCCTGAAAGGCAATTACTTGGAGG
TGGTGATCAGCAAAGTCAGTGCGCAAGAACCGCAACCGAAAAGTGTCTTAGAGAAAGAGGGCAAGCTGATTTCGATTAAC
TTCCAAGATATTCCCGTGCGCAACGTGCTGCAGCTTATCGCCGACTACAACCAGTTCAACTTGGTGGTCTCCGATTCCGT
CAGCGGCAACTTGACATTGCGCCTCGATGGCGTGCCGTGGCAACAAGTGCTGGATATCATTTTACAAGTCAAAGGGCTGG
ACAAACGGGTCGATGGCAACGTCATTCTGGTGGCGCCCAAAGAGGAGCTGGACCGACGCGAGCAGCAGCAATTGGAAAAA
GCGCGCATGGCGGAAGAGCTGGGCGATCTGCAATCGGAAATCATCAAAATCAATTTCGCCAAGGCATCCGACATTGCACA
GATGATTGGCGGCGATGGGGCGGTGAACATGTTGTCAGAGCGCGGCTCCATCAGTATTGATGAGCGCACCAATGCCTTGC
TACTGCGCGAGCTGCCTGACAACATTCACGTGATCCGCGACATCGTGGCTTCACTCGACATTCCGGTCAAACAAGTGCAA
ATCGAAGCGCGCATCGTCACGGTGAATGAGGGCGATTTGGATGAGCTTGGCGTGCGCTGGGGCTTTAGTTCCACCAACGG
GAGCACCACCACTGGTGGCTCGATTGAAAGTAATCTCGGTGCCATTGGGTTGTATGACGGCAGCAGTGATTCAGGCGATG
GCGCCGCGATTGATGATTTCCTCAATGTGAACCTTGCTGCTACCTCCGCCAATGCATCCAGTATTGCGTTTCAGGTGGCC
AAGTTGGGCGCTGACACCTTACTTGATCTTGAATTGTCGGCGATGCAGCGCGAGTCGAAAGCGGAAATTATTTCCAGCCC
AAGGCTGATCACCACCAACAAACAGCCGGCTTACATTGAACAGGGGACGGAAATCCCTTATCTCGAATCGTCCTCCAGTG
GCGCAACCAGCGTGGCATTTAAAAAAGCGGTCCTCAGCCTCAAAGTGACCCCGCAAATCACGCCTGACAACCGTTTGGTG
CTCGATTTGAGCGTCACCCAAGACCGACCGGGTGAAGTGGTGAAAACGGGCACGGGCGAAGCGGTGGCGATTAACACGCA
GCGTATTGGGACCCAAGTGTTAGTTAATAACGGTGAAACCGTGGTGCTGGGCGGCATTTTTCAACACAGCGTGACCAGTT
CTGTGGACAAAGTGCCCTTGCTTGGGGACTTGCCATTACTGGGCGCGCTGTTCCGTCGCAGCTACGAACAGATGGGGAAA
AGTGAGCTACTGATCTTCGTCACGCCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)

Secretin and TonB N terminus short domain (STN)

(179-227)

Bacterial type II and III secretion system protein (Secretin)

(414-572)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

80.729

100

0.81
  pilQ Vibrio cholerae strain A1552

80.729

100

0.81
  pilQ Vibrio campbellii strain DS40M4

77.413

95.645

0.74
  pilQ Pseudomonas aeruginosa PAK

39.86

99.652

0.397
  pilQ Legionella pneumophila strain ERS1305867

41.575

95.122

0.395
  pilQ Legionella pneumophila strain Lp02

41.575

95.122

0.395
  comQ Acinetobacter baylyi ADP1

38.737

100

0.395
  pilQ Acinetobacter baumannii D1279779

37.113

100

0.376
  pilQ Acinetobacter baumannii strain A118

37.113

100

0.376

Multiple sequence alignment