Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VFU_RS03105 Genome accession   NC_016602
Coordinates   653978..654982 (+) Length   334 a.a.
NCBI ID   WP_014204334.1    Uniprot ID   -
Organism   Vibrio furnissii NCTC 11218     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 648978..659982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VFU_RS03085 (vfu_A00625) - 649118..649381 (-) 264 WP_004728560.1 DUF3624 domain-containing protein -
  VFU_RS03090 (vfu_A00626) - 649505..650239 (-) 735 WP_004728561.1 glutathione peroxidase -
  VFU_RS03095 (vfu_A00627) oxyR 650383..651279 (+) 897 WP_014204332.1 DNA-binding transcriptional regulator OxyR -
  VFU_RS03100 (vfu_A00628) - 651353..653833 (-) 2481 WP_014204333.1 penicillin-binding protein 1A -
  VFU_RS03105 (vfu_A00629) pilM 653978..654982 (+) 1005 WP_014204334.1 type IV pilus assembly protein PilM Machinery gene
  VFU_RS03110 (vfu_A00630) pilN 654972..655562 (+) 591 WP_014204335.1 PilN domain-containing protein Machinery gene
  VFU_RS03115 (vfu_A00631) pilO 655555..656145 (+) 591 WP_014204336.1 type 4a pilus biogenesis protein PilO Machinery gene
  VFU_RS03120 (vfu_A00632) pilP 656135..656647 (+) 513 WP_041942470.1 pilus assembly protein PilP Machinery gene
  VFU_RS03125 (vfu_A00633) pilQ 656672..658396 (+) 1725 WP_041942471.1 type IV pilus secretin PilQ Machinery gene
  VFU_RS03130 (vfu_A00634) aroK 658584..659102 (+) 519 WP_004728569.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36823.15 Da        Isoelectric Point: 6.6617

>NTDB_id=42929 VFU_RS03105 WP_014204334.1 653978..654982(+) (pilM) [Vibrio furnissii NCTC 11218]
MGKSLVTGVDIGHHSIKAVVLKPKGESYSLVGYQEIVVSDDIFADNHTLDYQKIVKKLKELKKGLPLFSRKVALAVPDNA
VISKVLQIDSDLDASEQEFAVYQAFSHQSPFPIDDLSLDFVLSGDTPAGRSASTLYQVYATRREVVDNRLSVASKAGFEP
LLLDVQVHGLLQIWQWVSRAQQRDDWMLVDVGLTQTTLCMDFADKAPFYKDIPLGTRQWHADPTPHPVPLSEPPMAGVNE
LVERIARQVQLLLSLQGHQALGGIWLSGGGATTEGLSAALAARLNLPCELFHPLTGFVTRRRMRARVPDDLQRFSTAAGL
ALRGLNWLERDHGA

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=42929 VFU_RS03105 WP_014204334.1 653978..654982(+) (pilM) [Vibrio furnissii NCTC 11218]
ATGGGTAAATCATTAGTTACAGGTGTCGATATCGGCCACCACAGTATTAAAGCGGTGGTTTTAAAACCGAAGGGCGAATC
GTATTCGCTGGTCGGCTACCAAGAAATAGTCGTATCCGACGATATTTTCGCTGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGATTACCGCTATTTAGTCGCAAAGTTGCGCTGGCTGTGCCGGATAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGCGATTTAGATGCCAGCGAACAAGAATTTGCTGTCTATCAGGCGTTTTCCCA
TCAATCGCCGTTTCCCATCGATGATCTGAGTTTGGATTTCGTGTTAAGCGGCGATACCCCCGCTGGGCGCAGTGCCAGCA
CCCTGTATCAGGTGTACGCCACGCGACGCGAAGTCGTCGATAACCGGTTATCGGTGGCGAGCAAAGCGGGCTTTGAGCCG
CTGCTGCTCGATGTGCAGGTGCATGGGTTGCTCCAGATTTGGCAATGGGTGAGTCGCGCGCAGCAGCGCGACGATTGGAT
GCTGGTGGACGTCGGGCTGACGCAAACCACGTTATGCATGGATTTCGCCGATAAAGCGCCCTTCTATAAAGACATTCCGC
TGGGAACGCGTCAATGGCATGCCGACCCCACGCCCCATCCTGTGCCGCTGAGCGAGCCACCGATGGCTGGAGTCAACGAG
CTGGTTGAGCGAATTGCGCGCCAAGTGCAACTGCTGTTGTCCTTGCAGGGCCATCAAGCGCTGGGCGGGATTTGGCTGTC
GGGCGGTGGGGCGACGACTGAAGGCCTCAGCGCGGCTTTGGCTGCGCGACTGAATCTGCCGTGTGAACTGTTTCATCCGC
TGACGGGATTTGTGACGCGCCGCCGCATGCGAGCGCGTGTACCAGACGATCTGCAACGTTTTAGTACAGCGGCCGGCTTG
GCGCTGCGCGGACTCAACTGGTTGGAGCGTGACCATGGTGCATGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(239-324)

Type IV pilus assembly protein PilM; (PilM_2)

(8-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

64.307

100

0.653
  pilM Vibrio campbellii strain DS40M4

45.562

100

0.461

Multiple sequence alignment