Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G7083_RS08510 Genome accession   NZ_CP049913
Coordinates   1742913..1744601 (-) Length   562 a.a.
NCBI ID   WP_166014381.1    Uniprot ID   A0A6G8D4A9
Organism   Vibrio sp. HDW18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1737913..1749601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7083_RS08485 (G7083_08485) yacG 1739066..1739263 (-) 198 WP_166014376.1 DNA gyrase inhibitor YacG -
  G7083_RS08490 (G7083_08490) zapD 1739348..1740088 (-) 741 WP_166014377.1 cell division protein ZapD -
  G7083_RS08495 (G7083_08495) coaE 1740120..1740728 (-) 609 WP_166014378.1 dephospho-CoA kinase -
  G7083_RS08500 (G7083_08500) pilD 1740725..1741597 (-) 873 WP_166014379.1 A24 family peptidase Machinery gene
  G7083_RS08505 (G7083_08505) pilC 1741652..1742878 (-) 1227 WP_166014380.1 type II secretion system F family protein Machinery gene
  G7083_RS08510 (G7083_08510) pilB 1742913..1744601 (-) 1689 WP_166014381.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G7083_RS08515 (G7083_08515) - 1744602..1745066 (-) 465 WP_166015021.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  G7083_RS08520 (G7083_08520) nadC 1745286..1746185 (-) 900 WP_166014382.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G7083_RS08525 (G7083_08525) ampD 1746453..1747007 (+) 555 WP_166014383.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G7083_RS08530 (G7083_08530) fldB 1747101..1747619 (-) 519 WP_166014384.1 flavodoxin FldB -
  G7083_RS08535 (G7083_08535) xerD 1747874..1748752 (+) 879 WP_166015022.1 site-specific tyrosine recombinase XerD -
  G7083_RS08540 (G7083_08540) dsbC 1748769..1749530 (+) 762 WP_166014385.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62072.21 Da        Isoelectric Point: 5.3946

>NTDB_id=428612 G7083_RS08510 WP_166014381.1 1742913..1744601(-) (pilB) [Vibrio sp. HDW18]
MLTNLIAILRQAELISAAQQQAVLEHVSAADTSVPEALLELKIFHPQQLTEQLSHIFGLPETELNRYDYAELCQQLGLRE
LITRYQALPLAKQGNLLLLAVSDPTLLQAEEEFRFASGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAD
LVQIGADELQAIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQFRIRLRCDGILIETQQPASQLSRRLAARLKI
LAKLNIAERRLPQDGRIKLRLNADTAIDLRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLTALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTAQINISTAEDPVEINLTGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCQQCKVAHAPSAILQNQFAFQADE
VLYQANPAGCNECTGGYSGRVGIYEVMAFNPELAEAIMQRATIQQIEQLACANGMQTLQQSGLEKLRQGITSFTELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=428612 G7083_RS08510 WP_166014381.1 1742913..1744601(-) (pilB) [Vibrio sp. HDW18]
ATGCTCACCAATCTAATCGCGATCTTGCGCCAAGCTGAGCTAATTAGTGCGGCGCAACAGCAAGCGGTGCTCGAACACGT
TAGCGCTGCCGACACTTCGGTGCCGGAGGCGCTGCTGGAGCTAAAGATTTTCCACCCTCAGCAGTTAACCGAGCAACTGA
GCCATATTTTTGGCTTACCAGAAACCGAACTTAACCGCTACGACTATGCCGAGCTGTGCCAACAGCTCGGTTTACGTGAG
CTGATCACCCGCTATCAAGCACTGCCGCTCGCCAAACAAGGCAATTTACTGCTGCTCGCGGTGTCCGATCCAACCTTACT
GCAAGCCGAAGAAGAGTTTCGCTTCGCCTCTGGGCTACAAGTTGAGTTGGCACTGGCCGATCATCGTGCCTTGCAAGCGG
CAATCCGCCGTTTGTATGGCCGCTCAATTCAAGGCGCAGCTAATCAAGGTAAAGAGATCAGTCAAGATGAGCTGGCCGAT
CTGGTGCAAATTGGCGCCGATGAGCTGCAAGCGATTGAAGATCTGAGCCAAGATGACTCGCCGGTCAGCCGTTTTATTAA
CCAAGTGCTGCTTGATGCGGTGCGCAAAGGCGCCTCGGATATTCATTTTGAGCCGTATGAAAACCAATTTCGCATTCGGC
TACGCTGCGATGGGATTTTAATCGAAACCCAGCAACCGGCGAGCCAGTTAAGCCGCCGCTTAGCCGCGCGGCTAAAAATC
CTCGCCAAACTCAATATTGCCGAGCGCCGCCTACCGCAAGATGGGCGGATCAAACTGCGCCTTAATGCCGATACCGCAAT
TGATCTGCGCGTCTCAACCTTGCCCACGTTATGGGGTGAAAAAATCGTGCTGCGGCTGCTCGACAGCAGCGCGGCCAATT
TGGATATTGATAAGCTCGGTTACAACCCGCAGCAAAAACAGCTCTATCTAACCGCGCTAAAACGCCCACAAGGGATGATT
TTAATGACTGGCCCAACCGGCAGCGGCAAAACCGTATCGCTGTACACAGGGCTGCGCATTCTCAATACCGCGCAAATCAA
TATCTCCACCGCAGAAGATCCGGTCGAAATTAACCTGACGGGTATTAACCAAGTACAAGTGCAACCGAAAATTGGTTTTG
GCTTTGCCGAAGCACTGCGCTCATTTTTGCGCCAAGATCCCGATGTGGTGATGGTCGGCGAAATCCGCGATCTGGAAACC
GCAGAAATCGCGGTCAAAGCGGCGCAAACTGGTCACTTAGTGCTCTCAACTTTACATACCAACTCCGCGGCAGAAACCGT
GATCCGCTTAGCCAATATGGGAGTGGAGCCGTTTAACCTCGCCTCATCACTGAGTTTAATTATTGCTCAGCGCCTGGCGC
GTCGCTTATGCCAACAGTGCAAAGTGGCTCATGCGCCATCGGCGATTTTGCAAAACCAGTTTGCCTTTCAAGCCGATGAA
GTGTTGTACCAAGCCAATCCAGCCGGCTGCAATGAATGTACCGGTGGCTATTCTGGTCGGGTCGGCATTTATGAGGTGAT
GGCGTTTAACCCAGAGCTGGCAGAAGCGATTATGCAGCGCGCCACCATTCAACAAATTGAACAGCTAGCCTGCGCAAACG
GCATGCAAACCCTGCAACAGTCCGGCTTAGAAAAACTGCGCCAAGGCATCACCAGTTTTACCGAGCTACAACGAGTACTG
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8D4A9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

90.214

100

0.902
  pilB Vibrio campbellii strain DS40M4

72.954

100

0.73
  pilB Vibrio parahaemolyticus RIMD 2210633

72.242

100

0.722
  pilB Acinetobacter baumannii D1279779

49.12

100

0.496
  pilB Legionella pneumophila strain ERS1305867

49.362

97.687

0.482
  pilB Acinetobacter baylyi ADP1

47.576

99.11

0.472
  pilF Neisseria gonorrhoeae MS11

45.035

100

0.452
  pilB/pilB1 Synechocystis sp. PCC 6803

38.27

100

0.409
  pilB Haemophilus influenzae Rd KW20

39.655

92.883

0.368
  pilB Haemophilus influenzae 86-028NP

39.655

92.883

0.368
  pilF Thermus thermophilus HB27

41.344

87.367

0.361