Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   G5S34_RS01660 Genome accession   NZ_CP049139
Coordinates   364421..365557 (-) Length   378 a.a.
NCBI ID   WP_182994827.1    Uniprot ID   -
Organism   Herbaspirillum frisingense strain IAC152     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 359421..370557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5S34_RS01630 (G5S34_01630) mutT 359953..360366 (+) 414 WP_182993572.1 8-oxo-dGTP diphosphatase MutT -
  G5S34_RS01635 (G5S34_01635) yacG 360454..360639 (-) 186 WP_034336666.1 DNA gyrase inhibitor YacG -
  G5S34_RS01640 (G5S34_01640) zapD 360669..361424 (-) 756 WP_006713184.1 cell division protein ZapD -
  G5S34_RS01645 (G5S34_01645) coaE 361618..362238 (-) 621 WP_182993573.1 dephospho-CoA kinase -
  G5S34_RS01650 (G5S34_01650) pilD 362248..363111 (-) 864 WP_182993574.1 prepilin peptidase Machinery gene
  G5S34_RS01655 (G5S34_01655) - 363190..364434 (-) 1245 WP_182993575.1 type II secretion system F family protein -
  G5S34_RS01660 (G5S34_01660) pilF 364421..365557 (-) 1137 WP_182994827.1 GspE/PulE family protein Machinery gene
  G5S34_RS01665 (G5S34_01665) - 365679..366971 (-) 1293 WP_077235793.1 HlyC/CorC family transporter -
  G5S34_RS01670 (G5S34_01670) - 367129..368084 (-) 956 Protein_329 2-hydroxyacid dehydrogenase -
  G5S34_RS01675 (G5S34_01675) - 368081..368938 (-) 858 WP_077235791.1 IclR family transcriptional regulator -
  G5S34_RS01680 (G5S34_01680) - 369207..370292 (+) 1086 WP_006713198.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 40996.65 Da        Isoelectric Point: 9.4423

>NTDB_id=424886 G5S34_RS01660 WP_182994827.1 364421..365557(-) (pilF) [Herbaspirillum frisingense strain IAC152]
MQAQTPAVADDDVPVVRLLHKLLADAVGMGASDLHFEPFEHFFRVRLRVDGVLHELAQPPLTLRDKLATRLKILARLDIA
EKRLPQDGKMRLALERRSIDLRVSTLPTQFGEKIVLRILDTAQASFSIAELGYEPAQQEALLQAIARPHGLVLMTGPTGS
GKTVSLYACLQRLNRPGVNIATAEDPVEINLPGVNQVGINERAGLDFATALRAFLRQDPDVLMVGEIRDLETADIAVKAS
QTGHLVLSTLHTNDAPSTLTRLLNMGVPAYNIAASVSLIVAQRLVRRLCRCRRPAPGKPSTYVPRGCPACNQSGYRGRTG
IYQVMPVSAAMQALILAQAGTLELARQAQAEGIIDLRRAGLLKVLRGETSMGEILAGT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=424886 G5S34_RS01660 WP_182994827.1 364421..365557(-) (pilF) [Herbaspirillum frisingense strain IAC152]
ATGCAAGCTCAAACCCCGGCGGTGGCCGATGACGATGTCCCGGTAGTACGCCTGCTGCACAAGCTGCTGGCCGACGCCGT
CGGCATGGGTGCCTCCGACCTGCATTTCGAACCCTTCGAACACTTCTTCCGCGTACGCCTGCGCGTCGATGGCGTGCTGC
ACGAACTGGCGCAGCCACCGCTGACGCTGCGCGACAAGCTGGCTACCCGCCTGAAGATCCTGGCGCGTCTGGACATCGCC
GAAAAGCGCCTGCCCCAGGACGGCAAGATGCGCCTGGCACTGGAGCGGCGCAGCATCGACCTGCGCGTCTCCACCCTGCC
TACCCAGTTCGGCGAAAAGATCGTCCTGCGCATCCTCGATACCGCCCAGGCCAGCTTCTCCATCGCCGAACTGGGCTATG
AGCCGGCCCAGCAGGAAGCGCTGCTGCAAGCCATCGCCCGACCGCATGGACTGGTGCTGATGACCGGCCCTACCGGCTCG
GGCAAGACGGTCTCCCTGTACGCCTGCCTGCAGCGGCTCAACCGCCCCGGGGTCAACATCGCCACGGCCGAGGACCCGGT
GGAAATCAACCTGCCCGGCGTCAATCAGGTCGGCATCAACGAGCGCGCCGGCCTGGACTTCGCCACGGCGCTGCGCGCCT
TCCTGCGCCAGGACCCGGACGTGCTGATGGTGGGCGAAATCCGCGACCTGGAAACCGCCGACATCGCAGTCAAGGCATCC
CAGACCGGGCACCTGGTGCTCTCCACCCTGCATACCAACGACGCCCCTTCGACCCTCACGCGCCTGCTCAACATGGGGGT
GCCGGCCTACAACATCGCTGCGTCGGTGAGCCTGATCGTGGCGCAACGGCTGGTACGCAGGCTGTGCCGCTGTCGGCGTC
CGGCGCCGGGCAAGCCATCCACCTATGTCCCGCGAGGCTGCCCGGCCTGCAACCAGAGCGGCTATCGCGGCCGCACCGGC
ATCTACCAGGTGATGCCGGTCAGCGCCGCCATGCAGGCGCTGATCCTGGCCCAGGCCGGCACGCTGGAGCTGGCGCGTCA
GGCGCAGGCCGAGGGCATCATCGACCTGCGCCGCGCCGGCCTGCTCAAGGTCCTGCGCGGCGAAACCAGCATGGGAGAGA
TCCTTGCCGGCACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(22-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.041

100

0.577
  pilB Acinetobacter baylyi ADP1

55.381

100

0.558
  pilB Acinetobacter baumannii D1279779

54.427

100

0.553
  pilB Vibrio cholerae strain A1552

54.308

100

0.55
  pilB Vibrio parahaemolyticus RIMD 2210633

54.45

100

0.55
  pilB Vibrio campbellii strain DS40M4

54.188

100

0.548
  pilB Legionella pneumophila strain ERS1305867

50.129

100

0.516
  pilB Haemophilus influenzae 86-028NP

46.257

98.942

0.458
  pilB Haemophilus influenzae Rd KW20

45.989

98.942

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

42.065

100

0.442
  pilF Thermus thermophilus HB27

42.597

100

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.228

100

0.431
  pilB Glaesserella parasuis strain SC1401

48.742

84.127

0.41
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.895

100

0.402