Detailed information NTG0424885

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: G5S34_RS01650
• Genome accession: NZ_CP049139
• Coordinates: 362248..363111 (-)
• Length: 287 a.a.
• NCBI ID: WP_182993574.1
• Uniprot ID: -
• Organism: Herbaspirillum frisingense strain IAC152
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 357248..368111

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G5S34_RS01620 (G5S34_01620)	argJ	357654..358889 (+)	1236	WP_182993571.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
G5S34_RS01625 (G5S34_01625)	-	359078..359953 (+)	876	WP_006713179.1	ATP-binding protein	-
G5S34_RS01630 (G5S34_01630)	mutT	359953..360366 (+)	414	WP_182993572.1	8-oxo-dGTP diphosphatase MutT	-
G5S34_RS01635 (G5S34_01635)	yacG	360454..360639 (-)	186	WP_034336666.1	DNA gyrase inhibitor YacG	-
G5S34_RS01640 (G5S34_01640)	zapD	360669..361424 (-)	756	WP_006713184.1	cell division protein ZapD	-
G5S34_RS01645 (G5S34_01645)	coaE	361618..362238 (-)	621	WP_182993573.1	dephospho-CoA kinase	-
G5S34_RS01650 (G5S34_01650)	pilD	362248..363111 (-)	864	WP_182993574.1	prepilin peptidase	Machinery gene
G5S34_RS01655 (G5S34_01655)	-	363190..364434 (-)	1245	WP_182993575.1	type II secretion system F family protein	-
G5S34_RS01660 (G5S34_01660)	pilF	364421..365557 (-)	1137	WP_182994827.1	GspE/PulE family protein	Machinery gene
G5S34_RS01665 (G5S34_01665)	-	365679..366971 (-)	1293	WP_077235793.1	HlyC/CorC family transporter	-
G5S34_RS01670 (G5S34_01670)	-	367129..368084 (-)	956	Protein_329	2-hydroxyacid dehydrogenase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 30946.08 Da
• Isoelectric Point: 7.7223

>NTDB_id=424885 G5S34_RS01650 WP_182993574.1 362248..363111(-) (pilD) [Herbaspirillum frisingense strain IAC152]
MHFLEAYALPGNAALAGLAALLGLLVGSFLNVVIHRLPIMMLRETANFVAMERGEALPHQDRYDLWLPHSACPHCARPLA
PWHKLPVLSWVLLHGRCSFCQTRISARYPLVELLSALLFAVVAWRFGLGATGLAVMLCSAFLIALAFIDADTMLLPDDLT
LPLLWLGLLVNLNQRLVALPDAVLGAAAGYGVLWLIFWIFKLATGKDGLGYGDFKLMAALGAWLGWQALPLVLLLASVMG
ALLGLALMVGGRHQRGQAIPFGPCLAGAGLLALLLDGAWLNLTHFAY

Nucleotide

• Length: 864 bp

>NTDB_id=424885 G5S34_RS01650 WP_182993574.1 362248..363111(-) (pilD) [Herbaspirillum frisingense strain IAC152]
ATGCATTTCCTGGAAGCCTACGCCCTCCCCGGCAATGCCGCCCTGGCTGGCCTGGCGGCCCTGCTGGGCCTGCTGGTGGG
CAGCTTCCTGAACGTGGTAATCCACCGCCTGCCCATCATGATGCTGCGCGAGACCGCCAATTTCGTGGCCATGGAGCGCG
GCGAAGCCCTGCCGCATCAGGATCGCTACGACCTCTGGCTGCCCCATTCCGCCTGCCCGCACTGCGCCCGACCGCTGGCG
CCGTGGCACAAGCTGCCGGTGCTGAGCTGGGTGCTGCTGCACGGCCGTTGCAGCTTTTGCCAGACCAGGATCAGCGCGCG
CTATCCGCTGGTGGAGCTGCTGAGCGCGCTCCTGTTCGCCGTGGTGGCCTGGCGCTTCGGACTGGGTGCCACCGGGCTGG
CCGTCATGCTCTGCAGCGCCTTCCTGATTGCGCTCGCCTTCATCGATGCCGACACCATGCTGCTGCCCGATGACCTGACC
CTGCCGCTGCTGTGGCTGGGGCTGCTGGTGAACCTGAACCAGCGCCTGGTGGCGCTGCCCGATGCAGTGCTGGGCGCGGC
CGCCGGCTATGGCGTGCTGTGGCTGATCTTCTGGATCTTCAAGCTGGCCACCGGCAAGGACGGCCTGGGCTATGGCGACT
TCAAGCTGATGGCCGCCCTGGGCGCCTGGCTGGGTTGGCAGGCGCTGCCGCTGGTGCTGCTGCTGGCCTCGGTGATGGGC
GCCCTGCTGGGCCTGGCGCTGATGGTCGGCGGGCGCCACCAGCGCGGCCAGGCCATTCCCTTCGGCCCCTGCCTGGCCGG
CGCCGGCCTGCTGGCCCTGCTGCTGGATGGCGCCTGGCTGAACCTGACTCACTTCGCTTACTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.846	90.592	0.488
pilD	Neisseria gonorrhoeae MS11	47.97	94.425	0.453
pilD	Vibrio campbellii strain DS40M4	47.212	93.728	0.443
pilD	Acinetobacter baumannii D1279779	42.754	96.167	0.411
pilD	Acinetobacter nosocomialis M2	42.754	96.167	0.411