Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   NH8B_RS01355 Genome accession   NC_016002
Coordinates   313462..314562 (-) Length   366 a.a.
NCBI ID   WP_014085711.1    Uniprot ID   G2IVG5
Organism   Pseudogulbenkiania sp. NH8B     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 278087..312810 313462..314562 flank 652


Gene organization within MGE regions


Location: 278087..314562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH8B_RS01180 (NH8B_0246) - 278087..278362 (-) 276 WP_014085682.1 DUF5676 family membrane protein -
  NH8B_RS01185 (NH8B_0247) - 278484..278891 (-) 408 WP_014085683.1 hypothetical protein -
  NH8B_RS01190 (NH8B_0248) - 278956..279861 (-) 906 WP_008954782.1 LysR family transcriptional regulator -
  NH8B_RS01195 (NH8B_0249) - 279881..280696 (-) 816 WP_014085684.1 thiazole synthase -
  NH8B_RS01200 (NH8B_0250) pyk 280698..282134 (-) 1437 WP_014085685.1 pyruvate kinase -
  NH8B_RS01205 (NH8B_0251) - 282131..282550 (-) 420 WP_232503512.1 heme-binding protein -
  NH8B_RS01210 (NH8B_0252) - 282759..284021 (-) 1263 WP_014085687.1 glycerate kinase -
  NH8B_RS01215 (NH8B_0253) - 284041..284463 (-) 423 WP_008954788.1 DUF3830 family protein -
  NH8B_RS01220 (NH8B_0254) - 284643..285527 (-) 885 WP_014085688.1 2-hydroxy-3-oxopropionate reductase -
  NH8B_RS01225 (NH8B_0255) hyi 285672..286451 (-) 780 WP_014085689.1 hydroxypyruvate isomerase -
  NH8B_RS01230 (NH8B_0256) gcl 286479..288254 (-) 1776 WP_014085690.1 glyoxylate carboligase -
  NH8B_RS01235 (NH8B_0257) - 288873..289616 (+) 744 WP_014085691.1 aspartate/glutamate racemase family protein -
  NH8B_RS01240 (NH8B_0258) - 289761..291230 (+) 1470 WP_014085692.1 NCS1 family nucleobase:cation symporter-1 -
  NH8B_RS01245 (NH8B_0259) puuE 291295..292230 (+) 936 WP_041702783.1 allantoinase PuuE -
  NH8B_RS01250 (NH8B_0260) - 292318..292833 (-) 516 WP_014085694.1 AmiS/UreI family transporter -
  NH8B_RS01255 (NH8B_0261) - 292951..293454 (-) 504 WP_014085695.1 ureidoglycolate lyase -
  NH8B_RS01260 (NH8B_0262) alc 293451..294488 (-) 1038 WP_014085696.1 allantoicase -
  NH8B_RS01265 (NH8B_0263) - 294572..295903 (-) 1332 WP_014085697.1 C4-dicarboxylate transporter DctA -
  NH8B_RS01270 (NH8B_0265) - 296455..297126 (+) 672 WP_008954800.1 GntR family transcriptional regulator -
  NH8B_RS01275 (NH8B_0266) - 297343..298536 (-) 1194 WP_014085699.1 urate hydroxylase PuuD -
  NH8B_RS01280 (NH8B_0267) uraH 298539..298892 (-) 354 WP_008954802.1 hydroxyisourate hydrolase -
  NH8B_RS01285 (NH8B_0268) uraD 299011..299529 (+) 519 WP_041703219.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
  NH8B_RS01290 (NH8B_0269) - 299631..300377 (-) 747 WP_008954804.1 FadR/GntR family transcriptional regulator -
  NH8B_RS01295 (NH8B_0270) lhpI 300609..301547 (+) 939 WP_014085701.1 bifunctional Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2- carboxylate reductase -
  NH8B_RS01300 (NH8B_0271) - 301544..302788 (+) 1245 WP_014085702.1 FAD-binding oxidoreductase -
  NH8B_RS01305 (NH8B_0272) - 302872..303990 (+) 1119 WP_014085703.1 branched-chain amino acid ABC transporter substrate-binding protein -
  NH8B_RS01310 (NH8B_0273) - 304104..304598 (-) 495 WP_014085704.1 TlpA disulfide reductase family protein -
  NH8B_RS01315 (NH8B_0274) - 304718..305662 (+) 945 WP_014085705.1 carbohydrate kinase family protein -
  NH8B_RS01320 (NH8B_0275) gshA 305755..307038 (+) 1284 WP_014085706.1 glutamate--cysteine ligase -
  NH8B_RS01325 (NH8B_0276) gshB 307057..308001 (+) 945 WP_014085707.1 glutathione synthase -
  NH8B_RS01330 (NH8B_0277) - 307989..309056 (+) 1068 WP_014085708.1 FAD:protein FMN transferase -
  NH8B_RS01335 (NH8B_0278) - 309053..309430 (+) 378 WP_008954813.1 diacylglycerol kinase -
  NH8B_RS01340 (NH8B_0279) - 309681..310061 (-) 381 WP_014085709.1 hypothetical protein -
  NH8B_RS01345 (NH8B_0280) topA 310519..312810 (-) 2292 WP_014085710.1 type I DNA topoisomerase -
  NH8B_RS01350 (NH8B_0281) - 312961..313419 (-) 459 WP_008954816.1 DUF494 family protein -
  NH8B_RS01355 (NH8B_0282) dprA 313462..314562 (-) 1101 WP_014085711.1 DNA-processing protein DprA Machinery gene

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 37724.89 Da        Isoelectric Point: 5.5716

>NTDB_id=42359 NH8B_RS01355 WP_014085711.1 313462..314562(-) (dprA) [Pseudogulbenkiania sp. NH8B]
MSSERADWALLALTPGIGPVGVLRLIDTFGSAAAAASANLSQLQRCLGDEAARALAGRVATADVEAALAWADAPGCFLLT
LADHDYPTALAEAGHAPPLLFGRGRRELLARPMLAMVGSRHATPQGRQTAEEFAHGLAQAGYTIVSGLASGIDGAAHRGA
LGTAASTIAVIGTGIDRVYPASHRQLAHQLAEGGLILSEFPLGTAPLAHNFPRRNRIIAGLSRGCLVVEANVNSGSLITA
RLAAEVGREVLAIPGSIHNPQARGCHRLIKDGAKLVETVDDVLDEIGRPAPDAPGAASTNTAVADGTAAEVAETPLLVQL
GYDPVDVDSLAARLELTAGEVYAMLLELELEGRVASLPGGRFQRLS

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=42359 NH8B_RS01355 WP_014085711.1 313462..314562(-) (dprA) [Pseudogulbenkiania sp. NH8B]
ATGAGCAGCGAGCGCGCCGACTGGGCGCTGCTGGCGCTGACGCCGGGCATCGGCCCGGTCGGCGTGCTGCGCCTGATCGA
CACCTTCGGCAGCGCCGCCGCCGCAGCTTCGGCCAACCTTTCGCAGTTGCAGCGTTGCCTCGGCGACGAGGCGGCGCGCG
CCCTGGCGGGCCGCGTGGCGACCGCCGACGTGGAGGCGGCGCTGGCCTGGGCCGACGCGCCGGGCTGTTTCCTGCTGACC
CTGGCCGATCACGACTACCCCACAGCGCTGGCCGAGGCCGGCCACGCCCCGCCGCTGCTGTTCGGCCGGGGCCGGCGCGA
ATTGCTGGCGCGCCCGATGCTGGCCATGGTCGGCAGCCGCCACGCCACACCGCAGGGCCGGCAGACCGCCGAGGAGTTCG
CGCACGGCCTGGCGCAGGCCGGCTACACCATCGTCAGCGGGCTCGCCAGCGGCATCGACGGCGCCGCCCACCGTGGCGCG
CTGGGCACGGCGGCATCGACCATCGCGGTGATCGGCACCGGCATCGACCGGGTCTACCCGGCCAGCCACCGCCAGCTCGC
CCACCAGTTGGCCGAAGGCGGCCTGATCCTGTCGGAATTTCCGCTCGGCACGGCGCCGCTGGCGCACAACTTCCCGCGCC
GCAACCGCATCATCGCCGGGCTCAGCCGCGGCTGCCTGGTGGTCGAGGCCAACGTCAACTCCGGCTCGCTGATCACCGCG
CGCCTCGCCGCCGAGGTCGGGCGCGAGGTGCTGGCGATTCCCGGCTCGATCCACAACCCGCAGGCGCGCGGCTGCCACCG
CCTGATCAAGGACGGCGCCAAGCTGGTGGAAACGGTGGACGACGTGCTGGACGAAATCGGCCGCCCCGCCCCCGACGCAC
CAGGGGCGGCCAGCACCAACACGGCCGTGGCGGACGGCACAGCCGCGGAGGTGGCGGAGACGCCGCTGCTGGTCCAGCTG
GGCTACGATCCGGTCGACGTCGACAGCCTGGCGGCGCGGCTCGAGTTGACGGCGGGGGAGGTTTACGCGATGCTTCTTGA
GCTGGAACTGGAAGGCCGGGTGGCAAGCCTGCCGGGCGGTCGTTTCCAGCGCCTGTCCTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(310-360)

DNA recombination-mediator protein A (DNA_processg_A)

(74-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G2IVG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Neisseria meningitidis strain C311

48.849

100

0.522
  dprA Neisseria meningitidis MC58

48.849

100

0.522
  dprA Neisseria gonorrhoeae MS11

48.462

100

0.516
  dprA Neisseria gonorrhoeae strain FA1090

48.462

100

0.516
  dprA Vibrio campbellii strain DS40M4

43.889

98.361

0.432
  dprA Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.566

100

0.399
  dprA Vibrio cholerae strain A1552

39.726

99.727

0.396
  dprA Glaesserella parasuis strain SC1401

41.329

94.536

0.391
  dprA Acinetobacter baylyi ADP1

38.889

98.361

0.383
  dprA Acinetobacter baumannii strain A118

39.296

93.169

0.366
  dprA Acinetobacter baumannii D1279779

39.003

93.169

0.363
  dprA Legionella pneumophila strain ERS1305867

44.186

82.24

0.363

Multiple sequence alignment