Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GZH78_RS25445 Genome accession   NZ_CP048408
Coordinates   5677439..5678656 (+) Length   405 a.a.
NCBI ID   WP_134333457.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain DR397     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5672439..5683656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH78_RS25425 (GZH78_25435) - 5673961..5674257 (+) 297 WP_039771652.1 DUF2845 domain-containing protein -
  GZH78_RS25430 (GZH78_25440) - 5674425..5674781 (-) 357 WP_011335977.1 BON domain-containing protein -
  GZH78_RS25435 (GZH78_25445) - 5675078..5675509 (-) 432 WP_039771655.1 pilin -
  GZH78_RS25440 (GZH78_25450) pilB 5675736..5677436 (+) 1701 WP_163976499.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GZH78_RS25445 (GZH78_25455) pilC 5677439..5678656 (+) 1218 WP_134333457.1 type II secretion system F family protein Machinery gene
  GZH78_RS25450 (GZH78_25460) pilD 5678658..5679530 (+) 873 WP_074691471.1 A24 family peptidase Machinery gene
  GZH78_RS25455 (GZH78_25465) coaE 5679527..5680150 (+) 624 WP_074691472.1 dephospho-CoA kinase -
  GZH78_RS25460 (GZH78_25470) yacG 5680147..5680347 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -
  GZH78_RS25465 (GZH78_25475) - 5680353..5680568 (-) 216 WP_007959584.1 hypothetical protein -
  GZH78_RS25470 (GZH78_25480) - 5680634..5681323 (-) 690 WP_134333458.1 energy-coupling factor ABC transporter permease -
  GZH78_RS25475 (GZH78_25485) - 5681457..5682083 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -
  GZH78_RS25480 (GZH78_25490) - 5682080..5682607 (+) 528 WP_039771669.1 MOSC domain-containing protein -
  GZH78_RS25485 (GZH78_25495) - 5682659..5682832 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44030.68 Da        Isoelectric Point: 10.1456

>NTDB_id=421121 GZH78_RS25445 WP_134333457.1 5677439..5678656(+) (pilC) [Pseudomonas fluorescens strain DR397]
MAVKAAKISVYAWEGTDRKGSKVTGELSGQNPALVKAQLRKQGINPGKVRKKSASLLSFGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ESLKAKIKKAMTYPSAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVISLSEFMQQWWWAILGALVAAFFGTR
HALKKSQALRDRRDAWFLKLPLVGTLMFKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVSTGM
QLNFSMRSTGIFPNMAVQMTAIGEESGALDEMLDKVAGFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=421121 GZH78_RS25445 WP_134333457.1 5677439..5678656(+) (pilC) [Pseudomonas fluorescens strain DR397]
ATGGCGGTCAAGGCAGCGAAAATCAGTGTCTACGCCTGGGAAGGTACGGACCGCAAAGGCAGCAAGGTGACCGGCGAGTT
GAGCGGCCAGAACCCCGCACTGGTCAAGGCCCAATTGCGCAAGCAAGGCATCAACCCGGGCAAGGTACGCAAGAAATCCG
CCTCGCTGCTGAGCTTCGGCAAGCGCATCAAGGCCCAGGACATTGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTACCGCTGTTGCAGTCGTTCGACATCATTGGCGAAGGCTTCGAAAACCCGGCCATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAGGTCGCCGCCGGCAACAGCTTCGCCGCCGCGCTGCGCAAGAAGCCTCAGTATTTTGACGAGTTGTACT
GCAACCTGGTGGATGCCGGTGAACAGTCCGGCGCCCTCGACACCCTGCTGGAACGGGTCGCGACCTACAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAGGCCATGACCTACCCGTCGGCCGTAGTGCTGGTGGCGGCGGTGGTCACCGGGAT
TCTGCTGGTCAAGGTGGTGCCGCAGTTTCAGTCGGTGTTCTCCGGCTTCGGTGCCGAACTGCCGGCCTTCACGCTGATGG
TGATCAGCCTGTCCGAATTCATGCAGCAATGGTGGTGGGCGATTCTCGGTGCGCTGGTGGCCGCGTTTTTCGGCACCCGC
CATGCCCTGAAAAAATCCCAGGCCCTGCGCGATCGACGCGACGCCTGGTTTCTCAAGCTGCCGCTGGTGGGCACATTGAT
GTTCAAGTCCGCCGTGGCGCGGTTTGCCCGGACCCTGTCGACTACCTTCGCCGCCGGTGTGCCGCTGGTGGAAGCGCTGG
ACTCGGTGGCCGGTGCCACCGGCAACGTGGTGTTCAAGCGTGCCGTGCTGCGCATCCGCCAGGACGTCTCTACCGGCATG
CAGCTGAATTTCTCGATGCGTTCCACCGGCATCTTTCCGAACATGGCGGTGCAGATGACCGCCATCGGCGAAGAGTCCGG
CGCACTGGACGAGATGCTCGACAAGGTCGCGGGGTTCTATGAGGACGAAGTGGACAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTGATCGTCGGCGGTCTGGTGGTGGCGATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.062

100

0.751
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Acinetobacter baumannii D1279779

59.069

100

0.595
  pilC Legionella pneumophila strain ERS1305867

53.75

98.765

0.531
  pilC Vibrio cholerae strain A1552

44.697

97.778

0.437
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Thermus thermophilus HB27

38.903

99.012

0.385

Multiple sequence alignment