Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GZH78_RS25440 Genome accession   NZ_CP048408
Coordinates   5675736..5677436 (+) Length   566 a.a.
NCBI ID   WP_163976499.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain DR397     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5670736..5682436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GZH78_RS25420 (GZH78_25430) - 5671010..5673961 (+) 2952 WP_163976497.1 DUF748 domain-containing protein -
  GZH78_RS25425 (GZH78_25435) - 5673961..5674257 (+) 297 WP_039771652.1 DUF2845 domain-containing protein -
  GZH78_RS25430 (GZH78_25440) - 5674425..5674781 (-) 357 WP_011335977.1 BON domain-containing protein -
  GZH78_RS25435 (GZH78_25445) - 5675078..5675509 (-) 432 WP_039771655.1 pilin -
  GZH78_RS25440 (GZH78_25450) pilB 5675736..5677436 (+) 1701 WP_163976499.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GZH78_RS25445 (GZH78_25455) pilC 5677439..5678656 (+) 1218 WP_134333457.1 type II secretion system F family protein Machinery gene
  GZH78_RS25450 (GZH78_25460) pilD 5678658..5679530 (+) 873 WP_074691471.1 A24 family peptidase Machinery gene
  GZH78_RS25455 (GZH78_25465) coaE 5679527..5680150 (+) 624 WP_074691472.1 dephospho-CoA kinase -
  GZH78_RS25460 (GZH78_25470) yacG 5680147..5680347 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -
  GZH78_RS25465 (GZH78_25475) - 5680353..5680568 (-) 216 WP_007959584.1 hypothetical protein -
  GZH78_RS25470 (GZH78_25480) - 5680634..5681323 (-) 690 WP_134333458.1 energy-coupling factor ABC transporter permease -
  GZH78_RS25475 (GZH78_25485) - 5681457..5682083 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62839.86 Da        Isoelectric Point: 5.9461

>NTDB_id=421120 GZH78_RS25440 WP_163976499.1 5675736..5677436(+) (pilB) [Pseudomonas fluorescens strain DR397]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMAFLDLNCLDRETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHATGLEEMADVDLDG
LDIESVDDSKQDNLGGIDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPAGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=421120 GZH78_RS25440 WP_163976499.1 5675736..5677436(+) (pilB) [Pseudomonas fluorescens strain DR397]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAACAACTGGTCCAGGCCGAATTGCTCACGGAAAAGAGTGCCCAGCAGGC
CTGGCAGCAGGCGCAGCGCAATCGCCTGTCGCTGGTCAGTTACCTGGTGCAGAACAAACTGGTGAAGAGCTGGCAGGTCG
CCGAGATTGCCTCGGAGCATTTCGGCATGGCATTTCTCGACCTCAACTGTCTCGACAGGGAAACTCAGCCCAAGGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCCCTCTGGCGGCGCGGCAACAAACTGTTCGTGGGGATTTCCGA
CCCCAGCAATCATCAGGCGATCAATGACATTCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCACCGACGCCATCGAAAAATTCTTCGACACCCACGCCACCGGACTGGAGGAAATGGCCGATGTCGACCTTGACGGG
CTGGACATCGAGTCGGTCGACGACAGCAAACAGGACAACCTCGGCGGCATCGATGCCGACGATGCCCCGGTGGTGCGTTT
TGTTCACAAGATGCTGCTGGACGCGATCAAGAGCGGCTCGTCCGACCTGCACTTCGAGCCTTACGAAAAGAACTACCGGG
TGCGGGTGCGCACCGACGGCATTCTGCGGGAAGTGGCCAAGCCGCCGATTCAACTGGCCGGGCGCATCGCTGCACGCCTC
AAGGTAATGGCCAGCCTCGACATTTCGGAGCGACGCAAACCCCAGGACGGGCGGATCAAGATGCGTCTGTCGAAAAGCAA
GTCGATCGATTTCCGGGTCAACACCCTGCCCACCCTCTGGGGGGAGAAAGTGGTGATTCGGATCCTCGACCCGTCCAGCG
CCCAGATCGGCATCGATGCCCTCGGCTACGAGCCGGAACAGAAAGACCTGTACATGGCCGCCCTCAAACAGCCGCAAGGC
ATGATTCTGGTAACCGGCCCCACCGGTTCGGGCAAAACCGTGTCGCTGTACACGGGACTGAACATCCTCAATACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCCGTCAGG
GGTTGGACTTTGCCCAGGCCTTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAAATCCGCGACCTC
GAAACCGCCGAAATCGCGATCAAGGCCGCCCAGACCGGGCACCTTGTGCTGTCCACCCTGCACACCAACAGCGCGGCGGA
AACCCTCACCCGCCTGCACAACATGGGCATTCCCGGCTTCAACATCGCGACCTCAGTGAGCCTGATCATTGCCCAGCGTC
TGGCGCGAAAACTCTGCAGCCACTGCAAGAAACCCATCGAGATCCCCCGGGAGACCCTGATCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTATGAGCCCGCCGGTTGCGATCACTGCAACGGCGGGTACAAGGGTCGCGTGGGGAT
TTATGAAGTGGTAAAGAACACCCCCGATCTGCAACGGCTGATCATGGCCGAGGGCAACTCACTGGAAATCGATATCCAGA
TGCGCCGGGACGGCTTCAATGACCTGCGTACCTCGGGCCTGATCAAGGCCATGCAAGGCATTACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.025

100

0.581
  pilB Acinetobacter baumannii D1279779

57.672

100

0.578
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

49.554

99.117

0.491
  pilB Vibrio campbellii strain DS40M4

48.421

100

0.488
  pilF Neisseria gonorrhoeae MS11

49.023

99.47

0.488
  pilF Thermus thermophilus HB27

36.738

98.587

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.636

93.286

0.36

Multiple sequence alignment