Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GYM76_RS03410 Genome accession   NZ_CP048263
Coordinates   753509..754627 (+) Length   372 a.a.
NCBI ID   WP_220225901.1    Uniprot ID   -
Organism   Gilliamella sp. ESL0443     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 748509..759627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYM76_RS03390 (GYM76_03395) alr 749894..750967 (+) 1074 WP_220225897.1 alanine racemase -
  GYM76_RS03395 (GYM76_03400) mltG 750974..751987 (+) 1014 WP_370632608.1 endolytic transglycosylase MltG -
  GYM76_RS11075 - 752032..752355 (-) 324 WP_370632609.1 YqcC family protein -
  GYM76_RS11080 ampD 752359..752895 (-) 537 Protein_663 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GYM76_RS03405 (GYM76_03410) ppdD 753071..753502 (+) 432 WP_220225900.1 prepilin peptidase-dependent pilin -
  GYM76_RS03410 (GYM76_03415) pilB 753509..754627 (+) 1119 WP_220225901.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  GYM76_RS03415 (GYM76_03420) hofC 754634..755803 (+) 1170 WP_220225902.1 protein transport protein HofC -
  GYM76_RS03420 (GYM76_03425) - 755803..756558 (+) 756 WP_220225903.1 A24 family peptidase -
  GYM76_RS03425 (GYM76_03430) - 756736..757902 (+) 1167 WP_220225904.1 M20 aminoacylase family protein -
  GYM76_RS03430 (GYM76_03435) ygfZ 757957..758832 (-) 876 WP_220225905.1 tRNA-modifying protein YgfZ -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41356.33 Da        Isoelectric Point: 6.8155

>NTDB_id=420273 GYM76_RS03410 WP_220225901.1 753509..754627(+) (pilB) [Gilliamella sp. ESL0443]
MMQEDQLVESLDKLLLSALDKRASDIHFEPYQNYYRIRMRIDGVLQNRLIPPLSLAKQITARLKIMANLNIAEHRLPQDG
QLVINNYAMRIATLPVINGEKIVLRVMDNHESELTINDLGLTEQDLLTLKQILDYPQGLILVTGPTGSGKTMTLYSALKH
LNKAECNICCVEDPIEIPLEGINQTAINHKAGLTFSVILRALLRQDPDVMMIGEIRDHETAEIAIQAAQTGHLVLSTLHT
NSSVEAIVRLNQMGIKNYLLSSSLKLVIAQRLVRKLCQHCKVVASELTLIDNQPSPHFIAMGCDKCVGGYLGRVGVYELL
MVTPEVQQQIITNQPISPQNRVTLLQAGFKLVQQGITTLAELNRVLGEVVIG

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=420273 GYM76_RS03410 WP_220225901.1 753509..754627(+) (pilB) [Gilliamella sp. ESL0443]
ATGATGCAAGAAGATCAATTGGTTGAAAGTCTCGATAAGCTATTATTATCAGCACTGGATAAGCGAGCATCTGATATTCA
TTTCGAGCCTTATCAAAATTACTATCGAATACGCATGAGAATTGATGGCGTTTTGCAAAATAGGCTAATACCACCACTTA
GCCTTGCTAAGCAGATCACCGCCCGATTAAAAATTATGGCTAATTTAAATATTGCTGAACACCGCTTACCGCAAGATGGT
CAATTGGTAATTAATAACTATGCAATGCGAATTGCTACCTTGCCGGTGATCAATGGTGAAAAGATTGTATTGCGGGTTAT
GGATAATCATGAATCTGAGCTAACCATTAATGATTTAGGATTAACCGAACAAGATCTTCTCACACTTAAACAGATTTTAG
ATTATCCGCAAGGGTTGATTTTGGTTACTGGCCCAACCGGCAGTGGTAAAACTATGACTTTATATAGTGCGTTAAAGCAT
CTAAATAAAGCTGAATGTAATATTTGTTGTGTTGAAGATCCGATCGAAATACCTTTAGAAGGTATTAATCAAACTGCGAT
TAATCATAAAGCCGGTCTAACTTTTTCGGTGATATTACGCGCTTTATTGCGCCAAGATCCAGACGTGATGATGATTGGCG
AAATTCGTGACCATGAAACTGCCGAAATTGCCATTCAAGCAGCTCAAACAGGCCATTTAGTATTATCCACTTTGCATACT
AATTCGAGTGTTGAAGCAATAGTTCGGTTAAATCAAATGGGCATCAAAAATTATCTTTTATCGTCCAGTTTGAAATTGGT
TATCGCTCAACGCTTGGTACGAAAGCTCTGCCAACATTGCAAAGTCGTTGCTAGTGAACTAACCCTTATTGATAATCAAC
CTTCACCACACTTTATCGCCATGGGGTGTGACAAGTGTGTTGGTGGTTATTTAGGCCGAGTAGGTGTATATGAACTGTTA
ATGGTGACCCCAGAAGTGCAACAACAGATTATAACTAATCAACCAATCTCACCCCAAAATAGAGTCACATTACTGCAAGC
TGGCTTTAAGTTAGTACAACAAGGTATCACTACTTTGGCTGAACTAAATCGGGTTTTAGGTGAAGTTGTCATTGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

49.081

100

0.503
  pilB Vibrio cholerae strain A1552

47.927

100

0.497
  pilB Acinetobacter baumannii D1279779

45.876

100

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.974

100

0.476
  pilB Acinetobacter baylyi ADP1

45.361

100

0.473
  pilB Haemophilus influenzae 86-028NP

47.185

100

0.473
  pilB Haemophilus influenzae Rd KW20

46.917

100

0.47
  pilB Vibrio campbellii strain DS40M4

45.789

100

0.468
  pilB/pilB1 Synechocystis sp. PCC 6803

42.857

100

0.452
  pilB Glaesserella parasuis strain SC1401

44.022

98.925

0.435
  pilF Neisseria gonorrhoeae MS11

42.667

100

0.43
  pilF Thermus thermophilus HB27

42.659

97.043

0.414
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.633

100

0.406
  ctsE Campylobacter jejuni subsp. jejuni 81-176

40.217

98.925

0.398

Multiple sequence alignment