Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GWK36_RS13495 Genome accession   NZ_CP048029
Coordinates   2756421..2757668 (+) Length   415 a.a.
NCBI ID   WP_166271865.1    Uniprot ID   A0A6G7VFG7
Organism   Caldichromatium japonicum strain No.7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2751421..2762668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK36_RS13480 (GWK36_13465) acs 2751719..2753665 (+) 1947 WP_166271859.1 acetate--CoA ligase -
  GWK36_RS13485 (GWK36_13470) - 2753820..2754653 (+) 834 WP_166271861.1 NYN domain-containing protein -
  GWK36_RS13490 (GWK36_13475) pilB 2754690..2756405 (+) 1716 WP_166271863.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GWK36_RS13495 (GWK36_13480) pilC 2756421..2757668 (+) 1248 WP_166271865.1 type II secretion system F family protein Machinery gene
  GWK36_RS13500 (GWK36_13485) pilD 2757710..2758585 (+) 876 WP_166271867.1 A24 family peptidase Machinery gene
  GWK36_RS13505 (GWK36_13490) coaE 2758598..2759221 (+) 624 WP_246237585.1 dephospho-CoA kinase -
  GWK36_RS13515 (GWK36_13500) - 2759366..2759776 (-) 411 WP_166271869.1 winged helix-turn-helix domain-containing protein -
  GWK36_RS15600 - 2760475..2760859 (-) 385 Protein_2673 NAD-dependent epimerase/dehydratase family protein -
  GWK36_RS15605 - 2760885..2762288 (-) 1404 WP_246237586.1 NAD(P)(+) transhydrogenase (Re/Si-specific) subunit beta -
  GWK36_RS13525 (GWK36_13510) - 2762301..2762600 (-) 300 WP_166271871.1 proton-translocating transhydrogenase family protein -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 44828.95 Da        Isoelectric Point: 10.0228

>NTDB_id=419590 GWK36_RS13495 WP_166271865.1 2756421..2757668(+) (pilC) [Caldichromatium japonicum strain No.7]
MAVAVTKAKPTPKSDQANIFVWEGTDRRGARLKGEIRAPDIAAVRTDLRRQGVTPLKVKLKPKPLFSGGKKKITGQDLAV
FTRQLATMMAAGVPMVQAFDIVGRGHENPRMQDIILSIKQDIESGTALSVALSKYPLYFDDLVCSLVAAGEQAGVLDVLL
DKIATYKEKTESIKGKIKKALFYPAAVIVVAIGVSAVLLIFVIPQFKELFSSFGADLPAFTLMVLGLSDIVRQWWWAILG
VLGIAAYAFGSAYKRSRRLREAIDRLSLKIPVIGPILNKAAIARFARTLSTMFAAGVPLVEALQSVAGATGNAVYQEAVL
KMREGVATGQSLQLTMRQHELFPHMVIQMTAIGEESGALDAMLGKVADFYEEQVDNAVDSLSSLLEPLIMVVIGSLVGSL
VIAMYLPIFKLAAVV

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=419590 GWK36_RS13495 WP_166271865.1 2756421..2757668(+) (pilC) [Caldichromatium japonicum strain No.7]
ATGGCCGTCGCTGTGACCAAGGCCAAACCCACCCCCAAGAGCGATCAGGCAAACATCTTTGTGTGGGAGGGCACCGATCG
GCGCGGCGCCCGACTCAAAGGCGAGATCCGCGCCCCCGACATCGCTGCCGTGCGCACCGACCTGCGCCGGCAAGGGGTCA
CTCCGCTCAAGGTCAAGCTCAAACCCAAGCCGCTCTTTAGCGGTGGCAAAAAAAAGATCACGGGGCAGGACCTTGCGGTT
TTCACTCGTCAGCTTGCCACCATGATGGCGGCAGGTGTCCCCATGGTCCAGGCATTCGATATCGTCGGGCGCGGACACGA
AAACCCGCGGATGCAGGACATCATCCTGTCGATCAAGCAGGACATCGAGAGCGGCACCGCCCTTTCCGTAGCCCTATCCA
AATATCCGCTCTATTTCGATGACTTGGTTTGTAGTCTGGTCGCCGCCGGCGAGCAGGCGGGTGTGCTCGATGTCCTGCTC
GACAAGATCGCGACCTACAAGGAAAAGACCGAATCGATCAAGGGCAAGATCAAAAAGGCCCTCTTTTATCCGGCGGCGGT
CATCGTGGTCGCCATCGGCGTTAGCGCAGTCCTTTTGATCTTTGTTATCCCCCAGTTTAAGGAGCTCTTTTCCAGCTTTG
GCGCTGATCTCCCCGCCTTCACCCTCATGGTCTTAGGGCTGTCGGATATTGTACGCCAATGGTGGTGGGCCATCCTTGGT
GTCTTGGGGATCGCGGCCTATGCCTTTGGGTCGGCCTATAAGCGCTCCCGCCGCCTGCGTGAAGCGATCGATCGCTTAAG
CCTCAAGATCCCGGTCATCGGCCCGATCCTGAATAAAGCCGCGATCGCCCGCTTTGCCCGCACCCTCTCGACCATGTTCG
CTGCCGGCGTGCCCCTGGTTGAGGCCCTGCAATCGGTGGCTGGGGCGACGGGCAACGCCGTCTATCAGGAGGCGGTGCTC
AAGATGCGCGAAGGGGTGGCGACGGGCCAATCCCTGCAATTGACCATGCGTCAACACGAACTCTTCCCGCACATGGTCAT
TCAGATGACCGCAATCGGCGAGGAGTCAGGCGCGCTCGACGCGATGCTCGGTAAGGTCGCCGATTTTTATGAAGAACAGG
TGGACAACGCTGTCGATAGCTTAAGCAGCCTGCTCGAACCCCTGATCATGGTGGTCATCGGCAGTCTGGTCGGCAGCCTG
GTGATCGCTATGTATCTACCGATCTTCAAGCTCGCCGCCGTCGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(81-204)

Type II secretion system (T2SS), protein F (T2SSF)

(285-407)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G7VFG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

59.091

95.422

0.564
  pilC Pseudomonas stutzeri DSM 10701

56.328

97.108

0.547
  pilC Acinetobacter baumannii D1279779

54.106

99.759

0.54
  pilC Acinetobacter baylyi ADP1

55.779

95.904

0.535
  pilG Neisseria meningitidis 44/76-A

47.739

95.904

0.458
  pilG Neisseria gonorrhoeae MS11

47.739

95.904

0.458
  pilC Vibrio campbellii strain DS40M4

42.643

96.627

0.412
  pilC Vibrio cholerae strain A1552

41.919

95.422

0.4
  pilC Thermus thermophilus HB27

39.599

96.145

0.381

Multiple sequence alignment