Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GWK36_RS13490 Genome accession   NZ_CP048029
Coordinates   2754690..2756405 (+) Length   571 a.a.
NCBI ID   WP_166271863.1    Uniprot ID   -
Organism   Caldichromatium japonicum strain No.7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2749690..2761405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWK36_RS13475 (GWK36_13460) - 2750909..2751586 (+) 678 WP_166271857.1 exonuclease domain-containing protein -
  GWK36_RS13480 (GWK36_13465) acs 2751719..2753665 (+) 1947 WP_166271859.1 acetate--CoA ligase -
  GWK36_RS13485 (GWK36_13470) - 2753820..2754653 (+) 834 WP_166271861.1 NYN domain-containing protein -
  GWK36_RS13490 (GWK36_13475) pilB 2754690..2756405 (+) 1716 WP_166271863.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GWK36_RS13495 (GWK36_13480) pilC 2756421..2757668 (+) 1248 WP_166271865.1 type II secretion system F family protein Machinery gene
  GWK36_RS13500 (GWK36_13485) pilD 2757710..2758585 (+) 876 WP_166271867.1 A24 family peptidase Machinery gene
  GWK36_RS13505 (GWK36_13490) coaE 2758598..2759221 (+) 624 WP_246237585.1 dephospho-CoA kinase -
  GWK36_RS13515 (GWK36_13500) - 2759366..2759776 (-) 411 WP_166271869.1 winged helix-turn-helix domain-containing protein -
  GWK36_RS15600 - 2760475..2760859 (-) 385 Protein_2673 NAD-dependent epimerase/dehydratase family protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63465.98 Da        Isoelectric Point: 6.2152

>NTDB_id=419589 GWK36_RS13490 WP_166271863.1 2754690..2756405(+) (pilB) [Caldichromatium japonicum strain No.7]
MAKDQATFKPSGLAWRLVKEQLISESQMHAAYQEARAKGRTFVQHLVTENILEPRRIAVLASHEFGVPLLDLDAFDPGLM
PMNLVDERLITKHRALPLMRRGNRLFLALSDPTNEQAIEEIRFNTGLITEPIIVEDHKLGAAIDKTLHAQDTSLIGDFAD
ADLEKLQIEGGDDEDTENLDANIDEAPIVRYVNKILVDAITSGASDIHFEPFEKFFRIRFRQDGLLREVANPPLNIAGRL
VSRLKVMSRMNIAERRVPQDGRIKLKVSRTRDIDFRVNTLPTLYGEKVVLRILDSSAAQVGIDALGFEPEQRENFLKAIQ
KPYGMILVTGPTGSGKTVSLYTALNILNKPEINISTVEDPVEIQVAGINQVNLNPKSGLTFAEALRAFLRQDPDVIMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLTRLANMGVPPFNIASSVLLIMAQRLVRRLCIHCRQPLDLPRAAMLEE
GFTEEEINQGFTVYGPVGCEKCNKGYRGRIGIFQVMPISEATARLIMEGGNSLQLANQARREGISDLRQSGLRKVKAGIT
SLEEVNRVTRD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=419589 GWK36_RS13490 WP_166271863.1 2754690..2756405(+) (pilB) [Caldichromatium japonicum strain No.7]
ATGGCCAAGGATCAAGCCACCTTCAAACCCTCTGGGCTGGCCTGGCGACTGGTCAAGGAGCAGCTCATCTCCGAGTCCCA
GATGCACGCCGCCTATCAAGAGGCGCGCGCCAAGGGACGCACCTTCGTCCAACACCTGGTCACCGAAAATATCCTTGAGC
CGCGGCGCATCGCGGTCCTGGCCTCGCATGAGTTCGGCGTCCCCCTACTCGATCTGGATGCCTTCGATCCTGGACTCATG
CCTATGAACCTGGTCGATGAGCGCCTGATCACCAAACATCGCGCTCTGCCGCTGATGCGCCGCGGCAACCGACTATTCCT
GGCGCTGTCCGACCCTACCAATGAGCAGGCGATCGAAGAGATCCGGTTCAATACCGGTCTGATCACCGAACCCATCATCG
TCGAGGACCACAAGCTGGGCGCTGCCATCGATAAGACGCTCCATGCCCAGGATACCTCACTGATCGGCGACTTCGCTGAT
GCCGATCTGGAAAAGCTGCAGATCGAGGGCGGCGACGATGAGGATACCGAGAACCTGGATGCCAATATCGATGAGGCCCC
CATCGTCCGCTATGTCAACAAGATCCTGGTGGATGCCATCACCTCTGGCGCCTCGGATATCCATTTCGAGCCGTTTGAGA
AGTTCTTCCGCATCCGCTTCCGCCAGGATGGGCTGCTGCGTGAGGTCGCCAACCCGCCGCTCAATATCGCTGGCCGTCTG
GTCTCACGCCTGAAGGTCATGTCGCGCATGAATATCGCCGAGCGCCGGGTCCCCCAGGATGGTCGCATCAAGCTCAAGGT
CAGCCGTACGCGCGACATCGATTTCCGGGTCAATACCTTGCCGACCCTCTATGGCGAAAAGGTGGTACTGCGGATCCTGG
ACTCGAGTGCGGCCCAGGTGGGGATCGACGCCCTGGGCTTTGAGCCTGAACAACGCGAGAATTTCCTCAAGGCCATCCAA
AAACCATACGGCATGATCCTGGTCACTGGTCCCACCGGCTCAGGCAAGACCGTCTCGCTCTATACCGCCCTCAATATCCT
CAATAAACCCGAGATCAATATCTCGACGGTCGAGGACCCCGTCGAGATCCAGGTCGCAGGGATCAATCAGGTCAATCTCA
ATCCCAAGAGCGGGCTTACCTTCGCCGAGGCCCTGCGCGCCTTCTTACGTCAAGATCCCGATGTCATCATGGTCGGCGAG
ATCCGTGATCTCGAGACCGCCGAGATCGCGGTCAAGGCCGCCCAGACCGGTCACCTAGTGCTCTCGACCCTCCATACCAA
CGATGCCCCCCAAACCCTGACGCGTCTGGCCAACATGGGGGTGCCACCATTCAACATCGCCTCTTCGGTATTGTTGATCA
TGGCCCAGCGCCTGGTGCGCCGGCTGTGCATCCATTGCCGCCAGCCCCTCGATCTCCCGCGCGCCGCCATGCTCGAGGAA
GGCTTCACTGAGGAGGAGATCAATCAGGGATTTACCGTCTATGGACCCGTCGGCTGCGAAAAGTGCAACAAGGGCTACCG
CGGACGCATCGGTATCTTTCAGGTCATGCCGATCTCAGAGGCGACCGCCCGTCTGATCATGGAGGGGGGCAATTCGCTCC
AGCTCGCCAATCAAGCCAGGCGGGAAGGGATCTCAGATTTACGCCAATCTGGTCTGCGCAAGGTCAAGGCCGGGATCACC
AGCCTTGAAGAGGTCAACCGCGTCACTCGGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.652

100

0.56
  pilB Acinetobacter baumannii D1279779

55.846

100

0.56
  pilB Acinetobacter baylyi ADP1

56.028

98.774

0.553
  pilF Neisseria gonorrhoeae MS11

49.911

98.599

0.492
  pilB Vibrio cholerae strain A1552

50

97.023

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

48.128

98.249

0.473
  pilB Vibrio campbellii strain DS40M4

47.865

98.424

0.471
  pilF Thermus thermophilus HB27

40.143

97.723

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.768

96.673

0.375

Multiple sequence alignment