Detailed information NTG0419591

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GWK36_RS13500
• Genome accession: NZ_CP048029
• Coordinates: 2757710..2758585 (+)
• Length: 291 a.a.
• NCBI ID: WP_166271867.1
• Uniprot ID: A0A6G7VFR3
• Organism: Caldichromatium japonicum strain No.7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2752710..2763585

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GWK36_RS13485 (GWK36_13470)	-	2753820..2754653 (+)	834	WP_166271861.1	NYN domain-containing protein	-
GWK36_RS13490 (GWK36_13475)	pilB	2754690..2756405 (+)	1716	WP_166271863.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GWK36_RS13495 (GWK36_13480)	pilC	2756421..2757668 (+)	1248	WP_166271865.1	type II secretion system F family protein	Machinery gene
GWK36_RS13500 (GWK36_13485)	pilD	2757710..2758585 (+)	876	WP_166271867.1	A24 family peptidase	Machinery gene
GWK36_RS13505 (GWK36_13490)	coaE	2758598..2759221 (+)	624	WP_246237585.1	dephospho-CoA kinase	-
GWK36_RS13515 (GWK36_13500)	-	2759366..2759776 (-)	411	WP_166271869.1	winged helix-turn-helix domain-containing protein	-
GWK36_RS15600	-	2760475..2760859 (-)	385	Protein_2673	NAD-dependent epimerase/dehydratase family protein	-
GWK36_RS15605	-	2760885..2762288 (-)	1404	WP_246237586.1	NAD(P)(+) transhydrogenase (Re/Si-specific) subunit beta	-
GWK36_RS13525 (GWK36_13510)	-	2762301..2762600 (-)	300	WP_166271871.1	proton-translocating transhydrogenase family protein	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 31448.49 Da
• Isoelectric Point: 7.0175

>NTDB_id=419591 GWK36_RS13500 WP_166271867.1 2757710..2758585(+) (pilD) [Caldichromatium japonicum strain No.7]
MSWIELLDIHPVLLGALGLLLGLIVGSFLNVVIWRLPRILDAGWRRECAELLGLAAEGPAPPLSLAYPPSTCPHCGHRIR
IHENIPILSYLMLRGRCSACSSPIGIRYPLIEGLTALLTLIVVWAFGPTWPGAAALILTWTLIALAAIDLDTQLLPDAIT
QPMLWLGLILSLFGVFADSHSAIIGAVAGYLSLWTVFQLFRLTTGREGMGYGDFKLLAMLGAWLGWQYLPQILLLSAVVG
ALTGSLLILMRRHEAGRPLPFGPYLAAAGWISLMWGDAINVAYLQLSGLAG

Nucleotide

• Length: 876 bp

>NTDB_id=419591 GWK36_RS13500 WP_166271867.1 2757710..2758585(+) (pilD) [Caldichromatium japonicum strain No.7]
ATGAGCTGGATCGAATTGCTGGACATCCATCCTGTGCTGCTTGGTGCCCTGGGCCTGCTCCTGGGCCTGATCGTCGGCAG
TTTTCTCAATGTCGTCATCTGGCGTCTGCCGCGGATCCTGGACGCAGGCTGGCGGCGCGAATGTGCAGAACTCTTGGGAC
TCGCAGCCGAAGGACCTGCCCCCCCACTCAGCCTGGCCTATCCGCCTTCGACCTGTCCCCATTGCGGCCATCGCATCCGC
ATCCATGAGAACATCCCGATCCTCAGCTATCTCATGTTGCGCGGGCGCTGTTCGGCCTGCAGCTCACCGATTGGGATCCG
CTATCCGCTGATTGAGGGGTTGACCGCACTCCTGACCCTGATCGTCGTTTGGGCATTCGGACCGACCTGGCCAGGCGCAG
CGGCCCTGATCCTGACCTGGACCCTGATCGCGCTCGCTGCCATCGACCTCGATACCCAGCTCCTGCCCGATGCCATCACC
CAACCCATGCTCTGGCTGGGCCTGATCTTGAGCCTATTTGGGGTTTTTGCCGACAGTCATTCGGCGATCATCGGGGCGGT
CGCCGGTTATCTCAGCCTGTGGACGGTCTTTCAGCTGTTTCGCCTGACCACCGGCAGGGAGGGGATGGGCTATGGGGACT
TTAAGCTCTTGGCGATGCTTGGGGCCTGGCTGGGGTGGCAGTATCTGCCGCAGATCCTTTTGCTCTCTGCAGTGGTCGGT
GCCCTGACCGGCAGCCTCCTGATCCTGATGCGCCGTCATGAGGCGGGGCGCCCCCTGCCCTTTGGCCCTTATCTCGCTGC
TGCCGGTTGGATCAGTCTGATGTGGGGCGATGCGATCAATGTCGCCTATCTGCAGCTCAGCGGCTTGGCTGGATAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A6G7VFR3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.61	96.907	0.529
pilD	Vibrio campbellii strain DS40M4	51.799	95.533	0.495
pilD	Acinetobacter nosocomialis M2	50.746	92.096	0.467
pilD	Acinetobacter baumannii D1279779	50.373	92.096	0.464
pilD	Neisseria gonorrhoeae MS11	46.97	90.722	0.426