Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   I02_RS10415 Genome accession   NC_017651
Coordinates   2038465..2039475 (-) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli str. 'clone D i2'     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2033465..2044475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I02_RS10395 (i02_2086) mepM 2034432..2035754 (-) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  I02_RS10400 (i02_2087) znuA 2035770..2036702 (-) 933 WP_011076428.1 zinc ABC transporter substrate-binding protein ZnuA -
  I02_RS10405 (i02_2088) znuC 2036781..2037536 (+) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  I02_RS10410 (i02_2089) znuB 2037533..2038318 (+) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  I02_RS10415 (i02_2090) ruvB 2038465..2039475 (-) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  I02_RS10420 (i02_2091) ruvA 2039484..2040095 (-) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  I02_RS27270 yobI 2040234..2040299 (-) 66 WP_072123646.1 stress response small protein YobI -
  I02_RS10425 (i02_2092) yebB 2040371..2040973 (+) 603 WP_001024947.1 YebB family permuted papain-like enzyme -
  I02_RS10430 (i02_2093) ruvC 2040975..2041496 (-) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  I02_RS10435 (i02_2094) yebC 2041531..2042271 (-) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  I02_RS10440 (i02_2095) nudB 2042300..2042752 (-) 453 WP_077393227.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=41950 I02_RS10415 WP_000568522.1 2038465..2039475(-) (ruvB) [Escherichia coli str. 'clone D i2']
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=41950 I02_RS10415 WP_000568522.1 2038465..2039475(-) (ruvB) [Escherichia coli str. 'clone D i2']
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCGCAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTATTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAATATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCAATGCTCACTAACCTTGAACCACA
CGATGTACTGTTTATTGATGAGATCCACCGTCTTTCACCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGATTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGTGCGCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCTGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTACTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494

Multiple sequence alignment