Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CFU_RS20420 Genome accession   NC_015856
Coordinates   4604390..4606114 (+) Length   574 a.a.
NCBI ID   WP_014007897.1    Uniprot ID   -
Organism   Collimonas fungivorans Ter331     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4599390..4611114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFU_RS20405 (CFU_3920) ispB 4601816..4602766 (+) 951 WP_085959179.1 octaprenyl diphosphate synthase -
  CFU_RS20415 (CFU_3921) - 4602977..4604266 (+) 1290 WP_014007896.1 HlyC/CorC family transporter -
  CFU_RS20420 (CFU_3922) pilB 4604390..4606114 (+) 1725 WP_014007897.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CFU_RS20425 (CFU_3923) pilG 4606131..4607363 (+) 1233 WP_014007898.1 type II secretion system F family protein Machinery gene
  CFU_RS20430 (CFU_3924) pilD 4607363..4608229 (+) 867 WP_014007899.1 A24 family peptidase Machinery gene
  CFU_RS20435 (CFU_3925) coaE 4608236..4608889 (+) 654 WP_014007900.1 dephospho-CoA kinase -
  CFU_RS20440 (CFU_3926) zapD 4609020..4609775 (+) 756 WP_014007901.1 cell division protein ZapD -
  CFU_RS20445 (CFU_3927) yacG 4609797..4609985 (+) 189 WP_014007902.1 DNA gyrase inhibitor YacG -
  CFU_RS20450 (CFU_3928) mutT 4610005..4610418 (-) 414 WP_041742552.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62834.63 Da        Isoelectric Point: 5.1140

>NTDB_id=41949 CFU_RS20420 WP_014007897.1 4604390..4606114(+) (pilB) [Collimonas fungivorans Ter331]
MAAVFPNANSTSPISGLARALLQAGRLSVPQLEALHKKASESQTPFISALLESGQLDARSLALFCAETFGYPQLDLSVLN
LGMLPEKAIDAKLMETHSVLALAKRGNKIYVAFSDPTNIQALDQIKFQTELTVEPIIVEHPVLLKQIQKLVKSAEQSLNE
MVGDEQEINFVEEDAVAVADAAATDIDDAPVVRFLQKILTDAINQGASDLHFEPFEKFYRIRFRVDGVLRDMAQPPLSIK
EKLVSRIKVISKLDISEKRVPQDGRMRLAVSKTRAIDFRVSTLPTLFGEKVVMRILDPSQAQMGIDALGYDPDQKEILME
AIQRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGINISTAEDPAEINLPGVNQVNINDRAGLTFPVALKAFLRQDPDIIM
VGEIRDLETADISIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVPAFNIASSVILITAQRLARRLCSCKQPITVQEEALL
EAGFTEDDLDGSWMPYKAVGCDRCNGTGYKGRVGIYQIMPITEEIERIILAHGTALEIEAQAKREGVKTLRLSGLMKVKQ
GLTSLEEVLGCTNI

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=41949 CFU_RS20420 WP_014007897.1 4604390..4606114(+) (pilB) [Collimonas fungivorans Ter331]
ATGGCAGCAGTATTTCCCAACGCTAATTCCACCAGTCCGATCTCCGGACTTGCCCGCGCATTGCTGCAAGCCGGACGTCT
TTCCGTACCCCAGCTGGAGGCCTTGCACAAAAAGGCTAGCGAGAGTCAAACGCCGTTTATCTCCGCCTTGCTGGAAAGCG
GGCAGCTGGATGCCCGTTCGCTTGCCCTGTTTTGCGCCGAGACCTTCGGTTATCCGCAGCTGGACCTGAGCGTACTCAAC
CTCGGCATGCTGCCGGAAAAAGCCATCGACGCCAAGCTGATGGAAACCCATTCCGTATTGGCCCTGGCGAAACGCGGCAA
CAAGATCTATGTCGCCTTTTCCGATCCGACCAATATCCAGGCGCTGGACCAGATCAAGTTCCAGACCGAGCTGACGGTTG
AACCGATCATCGTCGAACACCCGGTGCTGCTGAAACAGATCCAGAAACTGGTCAAGTCAGCCGAGCAAAGCCTCAACGAA
ATGGTCGGCGACGAGCAGGAAATCAACTTCGTCGAAGAGGATGCGGTAGCGGTTGCCGATGCTGCGGCTACCGATATCGA
CGATGCGCCGGTAGTCAGGTTCTTGCAGAAGATACTGACCGACGCCATCAACCAGGGCGCGTCCGACTTGCATTTCGAGC
CGTTCGAAAAATTCTACCGTATCCGTTTCCGGGTCGACGGCGTCTTGCGCGACATGGCGCAGCCGCCGCTGTCGATCAAG
GAAAAACTGGTATCCCGCATCAAGGTGATCTCCAAGCTGGATATTTCCGAAAAGCGGGTGCCGCAAGATGGCCGCATGCG
CCTGGCGGTATCCAAGACCCGCGCAATCGATTTCCGGGTCAGCACATTGCCGACCTTGTTTGGCGAAAAAGTCGTAATGC
GTATCCTGGACCCGAGCCAGGCCCAGATGGGGATTGACGCGCTGGGTTACGACCCGGACCAGAAAGAAATCCTGATGGAG
GCTATCCAGCGTCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCCGGCAAAACCGTATCGCTGTATACCTGCCT
GAACATCCTGAACCAGCCTGGCATCAACATTTCCACCGCCGAAGATCCGGCCGAAATCAATTTGCCCGGCGTGAACCAGG
TCAACATCAACGACCGTGCCGGACTGACTTTCCCGGTGGCGCTGAAAGCCTTCCTGCGGCAGGATCCCGACATCATCATG
GTCGGTGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCCGCGCAAACCGGGCACATGGTGTTTTCCACACT
GCACACCAACGATGCGCCATCGACGCTGACGCGTCTGATGAACATGGGGGTGCCGGCCTTCAATATCGCTTCGTCGGTGA
TCCTGATCACTGCGCAACGGCTGGCGCGCCGCCTGTGCAGCTGCAAGCAGCCGATTACTGTCCAGGAAGAAGCCTTGCTG
GAAGCCGGCTTTACCGAAGACGACCTCGACGGCAGCTGGATGCCGTACAAGGCGGTCGGCTGCGATCGCTGCAACGGCAC
AGGATACAAAGGGCGGGTCGGCATCTACCAAATCATGCCGATCACCGAAGAAATAGAACGGATTATCCTGGCGCACGGCA
CGGCGCTGGAAATCGAAGCGCAGGCTAAGCGCGAGGGGGTCAAGACCCTGCGTTTGTCAGGATTGATGAAGGTTAAACAA
GGGTTGACCAGCCTCGAAGAAGTGCTTGGCTGCACCAATATCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.816

99.477

0.545
  pilB Acinetobacter baylyi ADP1

53.739

100

0.538
  pilF Neisseria gonorrhoeae MS11

53.792

98.78

0.531
  pilB Legionella pneumophila strain ERS1305867

52.407

94.077

0.493
  pilB Vibrio cholerae strain A1552

47.163

98.258

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

46.903

98.432

0.462
  pilB Vibrio campbellii strain DS40M4

44.876

98.606

0.443
  pilF Thermus thermophilus HB27

40.449

93.031

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.414

92.683

0.375

Multiple sequence alignment