Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GWG10_RS02250 Genome accession   NZ_CP047983
Coordinates   453828..455069 (-) Length   413 a.a.
NCBI ID   WP_180911331.1    Uniprot ID   -
Organism   Aeromonas caviae strain 1605-27183     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 448828..460069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWG10_RS02220 (GWG10_02220) - 449624..450808 (+) 1185 WP_180911329.1 tetratricopeptide repeat protein -
  GWG10_RS02225 (GWG10_02225) mutT 450817..451221 (+) 405 WP_174213873.1 8-oxo-dGTP diphosphatase MutT -
  GWG10_RS02230 (GWG10_02230) yacG 451277..451471 (-) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  GWG10_RS02235 (GWG10_02235) zapD 451482..452204 (-) 723 WP_010675825.1 cell division protein ZapD -
  GWG10_RS02240 (GWG10_02240) coaE 452242..452856 (-) 615 WP_180911330.1 dephospho-CoA kinase -
  GWG10_RS02245 (GWG10_02245) pilD 452875..453750 (-) 876 WP_103261555.1 prepilin peptidase Machinery gene
  GWG10_RS02250 (GWG10_02250) pilC 453828..455069 (-) 1242 WP_180911331.1 type II secretion system F family protein Machinery gene
  GWG10_RS02255 (GWG10_02255) pilB 455197..456903 (-) 1707 WP_180911332.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  GWG10_RS23145 (GWG10_02260) - 456907..457452 (-) 546 WP_180911333.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GWG10_RS02265 (GWG10_02265) nadC 457763..458626 (-) 864 WP_041213995.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GWG10_RS02270 (GWG10_02270) - 458630..459100 (-) 471 WP_042015101.1 TIGR02281 family clan AA aspartic protease -
  GWG10_RS02275 (GWG10_02275) ampD 459229..459801 (+) 573 WP_180911334.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45467.37 Da        Isoelectric Point: 9.8576

>NTDB_id=418993 GWG10_RS02250 WP_180911331.1 453828..455069(-) (pilC) [Aeromonas caviae strain 1605-27183]
MATLAQKRNAPKKVFSFRWHGVNRKGQKVSGELQADSINTVKAELRKQGVNVTRVSKQSQGLFSKGGARIKPMDIAVISR
QITTMLSAGVPLVQSLQIIARGHEKAAVRELIGQIAADVETGTPLSEALHRHPRYFDDLYCDLVEAGEQSGALETIYDRI
AIYREKNEALKSKIKKAMFYPSMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPAFTRFVIAISRFMQEWWYAIFGGAA
LAVFLYVRAWRKSQKVRDNTDKFILTIPVVGNILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTATMAIR
NEVVAGMQINVAMRTVDLFPDMVTQMVMIGEESGAIDDMLSKVAAIFEQEVDDMVDGLTSLLEPIIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=418993 GWG10_RS02250 WP_180911331.1 453828..455069(-) (pilC) [Aeromonas caviae strain 1605-27183]
ATGGCTACATTAGCTCAAAAACGCAACGCACCAAAAAAGGTCTTCTCCTTCCGCTGGCACGGCGTCAACCGCAAGGGACA
GAAGGTCTCCGGCGAGCTGCAAGCCGACAGCATCAATACCGTCAAGGCGGAACTGCGCAAGCAGGGCGTCAATGTCACCA
GGGTCAGCAAGCAGAGCCAGGGGCTCTTCTCCAAGGGCGGCGCCCGGATCAAGCCGATGGATATCGCCGTCATCTCCCGC
CAGATCACCACCATGCTCTCCGCCGGCGTGCCCCTGGTACAGAGCCTGCAGATCATCGCCCGAGGCCATGAAAAGGCGGC
GGTGCGCGAACTGATCGGCCAGATCGCCGCCGATGTGGAGACAGGTACCCCGCTGTCCGAGGCGTTGCACCGTCACCCCC
GTTACTTCGACGATCTCTATTGCGACCTGGTCGAGGCCGGGGAGCAATCCGGTGCCCTGGAGACCATCTACGACCGTATC
GCCATCTACCGCGAGAAGAACGAAGCCCTCAAATCCAAGATCAAGAAGGCCATGTTCTATCCGTCCATGGTCATTCTGGT
GGCCATCGTCGTCACCTCCATCCTGCTGCTCTTCGTCATTCCCCAGTTCGAGGATATCTTCAAGAGCTTCGGTGCCGAGC
TGCCCGCCTTTACCCGGTTCGTCATCGCCATCTCCCGCTTCATGCAGGAGTGGTGGTACGCCATCTTTGGCGGCGCGGCT
CTCGCCGTCTTCCTCTATGTGCGGGCCTGGCGAAAATCCCAGAAGGTCAGGGACAACACGGACAAGTTCATCCTCACCAT
TCCCGTGGTGGGCAACATACTGCACAAGGCGGCCATGGCCCGTTTTGCACGCACCCTCTCCACCACCTTCTCCGCCGGTA
TCCCACTGGTGGATGCCCTGGTCTCGGCGGCCGGAGCATCGGGCAACTATGTCTATCGCACTGCGACCATGGCCATTCGC
AACGAGGTGGTGGCCGGCATGCAGATCAACGTCGCCATGCGCACCGTGGATCTTTTCCCCGACATGGTGACCCAGATGGT
GATGATCGGTGAGGAATCCGGCGCCATCGACGACATGCTCTCCAAGGTCGCCGCCATTTTCGAACAGGAGGTGGACGACA
TGGTCGACGGCCTCACCAGCCTGCTCGAACCCATCATCATGGTGGTGCTCGGGGTGCTGGTCGGCGGCATGGTCGTGGCC
ATGTACCTCCCCATTTTCAAGCTGGGCTCTGTGATACACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.566

95.884

0.542
  pilC Legionella pneumophila strain ERS1305867

52.346

98.063

0.513
  pilC Acinetobacter baumannii D1279779

51.87

97.094

0.504
  pilC Acinetobacter baylyi ADP1

51.378

96.61

0.496
  pilC Vibrio cholerae strain A1552

46.867

96.61

0.453
  pilC Vibrio campbellii strain DS40M4

44.612

96.61

0.431
  pilG Neisseria meningitidis 44/76-A

40.988

98.063

0.402
  pilG Neisseria gonorrhoeae MS11

40.988

98.063

0.402
  pilC Thermus thermophilus HB27

38.5

96.852

0.373

Multiple sequence alignment