Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GWG10_RS02255 Genome accession   NZ_CP047983
Coordinates   455197..456903 (-) Length   568 a.a.
NCBI ID   WP_180911332.1    Uniprot ID   -
Organism   Aeromonas caviae strain 1605-27183     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450197..461903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWG10_RS02225 (GWG10_02225) mutT 450817..451221 (+) 405 WP_174213873.1 8-oxo-dGTP diphosphatase MutT -
  GWG10_RS02230 (GWG10_02230) yacG 451277..451471 (-) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  GWG10_RS02235 (GWG10_02235) zapD 451482..452204 (-) 723 WP_010675825.1 cell division protein ZapD -
  GWG10_RS02240 (GWG10_02240) coaE 452242..452856 (-) 615 WP_180911330.1 dephospho-CoA kinase -
  GWG10_RS02245 (GWG10_02245) pilD 452875..453750 (-) 876 WP_103261555.1 prepilin peptidase Machinery gene
  GWG10_RS02250 (GWG10_02250) pilC 453828..455069 (-) 1242 WP_180911331.1 type II secretion system F family protein Machinery gene
  GWG10_RS02255 (GWG10_02255) pilB 455197..456903 (-) 1707 WP_180911332.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  GWG10_RS23145 (GWG10_02260) - 456907..457452 (-) 546 WP_180911333.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GWG10_RS02265 (GWG10_02265) nadC 457763..458626 (-) 864 WP_041213995.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GWG10_RS02270 (GWG10_02270) - 458630..459100 (-) 471 WP_042015101.1 TIGR02281 family clan AA aspartic protease -
  GWG10_RS02275 (GWG10_02275) ampD 459229..459801 (+) 573 WP_180911334.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GWG10_RS02280 (GWG10_02280) pdhR 460078..460842 (+) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62527.94 Da        Isoelectric Point: 5.8224

>NTDB_id=418994 GWG10_RS02255 WP_180911332.1 455197..456903(-) (pilB) [Aeromonas caviae strain 1605-27183]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIAKLMESEQDVLGVDHIDEAEI
SELEVSDENSRLDEGVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNLDQLGFDERQKGQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=418994 GWG10_RS02255 WP_180911332.1 455197..456903(-) (pilB) [Aeromonas caviae strain 1605-27183]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCAGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCAAAGCCCTCG
CCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTTGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATCGAGAAGCACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTCGGCTTCAGCTTCGGCCTGCACACCGAAGCGCTGCTGGTCGAGGAGAGCA
AGCTGACCGCCGCCATCGCCAAGCTGATGGAGAGCGAGCAGGACGTGCTCGGCGTGGATCATATCGACGAGGCCGAGATC
TCCGAGCTCGAGGTGTCCGATGAGAACTCGCGTCTCGACGAGGGCGTCAACACGGCCGACGACGACGCCCCCATCGTCAA
GTACATCCACAAGATCATGATGGACGCCATCAAGCGCGGCGCATCGGACCTGCATTTCGAACCTTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAATCGCAACCCCGCCGGTCAATCTGGCCAACCGCTTCGCAGCCCGC
CTCAAGGTGATGGCCAGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGACGGCCGGATCAAGCTCAAGATTTCACGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAGAAAATCGTCATCCGCTTGCTGGACTCCTCTG
CCGCCCGTCTCAACCTCGATCAGCTCGGCTTCGACGAACGCCAGAAAGGGCAGTACCTTCACGCCCTCTCCAAGCCGCAG
GGGATGATCCTGGTCACCGGCCCCACAGGGTCGGGCAAGACGGTATCGCTCTATACCGGCCTCAACATCCTCAACACCAG
CGAGGTCAACATCTCCACCGCCGAGGACCCGGTAGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCCTGCGCTCCTTCCTGCGTCAGGACCCCGACATCGTCATGGTAGGCGAAATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCTACTCTGCACACCAATTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCCTCGGTAACCCTCATCATGGCCCAGC
GTCTCGCACGCAAACTGTGCGATCACTGCAAGGCGCCCGAGGTGGTGCCGGAAGCCGAGCTGCTGGAGCTGGGCTTTACC
CAGCAGCAACTGGCCGCCGGCTTGCGACTGTTCAAACCCGTGGGGTGCAAGGAGTGCTCGGGAGGCTACCGAGGGCGGGT
CGGGATCTATGAGATCATGCTGATGTCAGACAACATCGCCAAGCTCATCATGCAAGGAGCCAACTCGCTGCAAATCGCGG
CCATCGCCCAAAAGGAAGGGATGCGCACCCTGCGCACCTCCGGCCTTGAAAAAGCCCGCCTGGGAGTCACCAGCCTGGCC
GAAATAAACAGGATCACTACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.026

99.824

0.549
  pilB Acinetobacter baylyi ADP1

55.24

99.12

0.548
  pilB Legionella pneumophila strain ERS1305867

54.336

99.472

0.54
  pilB Vibrio cholerae strain A1552

52.88

100

0.533
  pilB Vibrio campbellii strain DS40M4

50.353

99.648

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50.089

99.12

0.496
  pilF Neisseria gonorrhoeae MS11

46.208

99.824

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.156

97.359

0.371
  pilF Thermus thermophilus HB27

39.962

92.958

0.371

Multiple sequence alignment