Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GWI30_RS18990 Genome accession   NZ_CP047962
Coordinates   4123366..4124607 (+) Length   413 a.a.
NCBI ID   WP_161507567.1    Uniprot ID   A0AAE6SNL4
Organism   Aeromonas media strain MC64     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4118366..4129607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWI30_RS18965 (GWI30_19105) ampD 4118716..4119288 (-) 573 WP_161507563.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GWI30_RS18970 (GWI30_19110) - 4119434..4119895 (+) 462 WP_374393893.1 retropepsin-like aspartic protease family protein -
  GWI30_RS18975 (GWI30_19115) nadC 4119899..4120762 (+) 864 WP_161507565.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GWI30_RS18980 (GWI30_19120) - 4121075..4121530 (+) 456 WP_374393832.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GWI30_RS18985 (GWI30_19125) pilB 4121530..4123236 (+) 1707 WP_161507566.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  GWI30_RS18990 (GWI30_19130) pilC 4123366..4124607 (+) 1242 WP_161507567.1 type II secretion system F family protein Machinery gene
  GWI30_RS18995 (GWI30_19135) pilD 4124688..4125560 (+) 873 WP_161507568.1 prepilin peptidase Machinery gene
  GWI30_RS19000 (GWI30_19140) coaE 4125579..4126193 (+) 615 WP_161507569.1 dephospho-CoA kinase -
  GWI30_RS19005 (GWI30_19145) zapD 4126231..4126953 (+) 723 WP_161507570.1 cell division protein ZapD -
  GWI30_RS19010 (GWI30_19150) yacG 4126963..4127157 (+) 195 WP_042649030.1 DNA gyrase inhibitor YacG -
  GWI30_RS19015 (GWI30_19155) mutT 4127233..4127637 (-) 405 WP_161507571.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45521.58 Da        Isoelectric Point: 10.1464

>NTDB_id=418801 GWI30_RS18990 WP_161507567.1 4123366..4124607(+) (pilC) [Aeromonas media strain MC64]
MATLAQKRNTPKKVFSFRWHGFNRKGQKVSGEFQADSINTVKAELRKQGVNVTKVSKQGQGLFSKGGARIKPMDIAVISR
QITTMLSAGVPLVQSLQIIARGHEKAAVRELIGQIATDVETGTPLSEALRRHPRHFDALYCDLVDAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPAMVILVAIVVTSILLLFVIPQFEEIFKSFGAELPVFTRFVIGISRFMQAWWYIIFGGTA
LAIFLYVRAWRKSQKVRDNTDRFILTIPVVGNILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTAILAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVAAIFEQEVDDMVDGLTSLLEPIIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=418801 GWI30_RS18990 WP_161507567.1 4123366..4124607(+) (pilC) [Aeromonas media strain MC64]
ATGGCAACATTAGCGCAAAAACGTAACACACCTAAAAAGGTCTTCTCCTTCCGCTGGCATGGATTCAATCGCAAGGGACA
AAAGGTTTCCGGTGAGTTCCAGGCCGACAGCATCAACACCGTCAAGGCGGAGCTGCGCAAGCAGGGCGTCAATGTCACCA
AGGTCAGCAAGCAGGGCCAGGGCCTGTTTTCCAAGGGGGGGGCTCGGATCAAGCCGATGGACATCGCCGTCATCTCCCGC
CAGATCACCACCATGCTCTCGGCCGGTGTACCGCTGGTGCAAAGCTTGCAGATCATCGCCCGCGGTCATGAGAAGGCGGC
CGTACGCGAACTCATCGGCCAGATCGCCACCGATGTGGAAACCGGCACCCCGCTGTCGGAGGCGCTGCGCCGCCACCCCC
GCCACTTCGATGCGCTCTACTGCGATCTGGTAGACGCGGGGGAGCAGTCCGGCGCGCTGGAAACCATCTACGACCGGATT
GCCACATACCGCGAGAAGAGTGAGGCGCTCAAGTCCAAGATCAAGAAGGCCATGTTCTATCCCGCCATGGTCATCCTGGT
CGCCATCGTCGTGACCTCCATCCTGCTGCTGTTCGTCATTCCCCAGTTCGAGGAGATCTTCAAGAGCTTCGGGGCAGAGC
TGCCAGTCTTCACCCGATTCGTCATCGGTATCTCCCGCTTCATGCAAGCTTGGTGGTATATCATCTTTGGTGGCACAGCC
TTGGCCATCTTCCTCTATGTGCGCGCCTGGCGAAAATCCCAGAAGGTGCGGGACAACACCGACAGATTCATCCTCACCAT
TCCGGTCGTGGGCAACATATTGCACAAGGCGGCCATGGCCCGTTTCGCCCGTACCCTCTCCACCACCTTCTCCGCCGGGA
TACCTCTGGTGGATGCCTTGGTGTCAGCGGCCGGCGCCTCTGGCAACTATGTCTATCGCACGGCCATCCTGGCCATTCGC
AACGAGGTGGTGGCCGGCATGCAGATCAACGTCGCCATGCGCACCGTGGATCTCTTCCCAGACATGGTGATCCAGATGGT
GATGATTGGTGAAGAGTCAGGCGCCATCGATGACATGCTCTCCAAGGTTGCCGCCATCTTCGAGCAGGAGGTGGACGACA
TGGTCGATGGCCTCACCAGCCTGCTCGAACCCATCATCATGGTAGTGCTCGGGGTGCTGGTCGGTGGCATGGTCGTCGCC
ATGTACCTCCCCATCTTCAAGCTGGGCTCTGTGATACACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.566

95.884

0.542
  pilC Legionella pneumophila strain ERS1305867

53.086

98.063

0.521
  pilC Acinetobacter baumannii D1279779

51.372

97.094

0.499
  pilC Acinetobacter baylyi ADP1

50.882

96.126

0.489
  pilC Vibrio cholerae strain A1552

48

96.852

0.465
  pilC Vibrio campbellii strain DS40M4

46.482

96.368

0.448
  pilG Neisseria gonorrhoeae MS11

40.05

97.337

0.39
  pilG Neisseria meningitidis 44/76-A

39.801

97.337

0.387
  pilC Thermus thermophilus HB27

39

96.852

0.378

Multiple sequence alignment