Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GWI30_RS18985 Genome accession   NZ_CP047962
Coordinates   4121530..4123236 (+) Length   568 a.a.
NCBI ID   WP_161507566.1    Uniprot ID   A0AAE6SLJ4
Organism   Aeromonas media strain MC64     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4116530..4128236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GWI30_RS18960 (GWI30_19100) pdhR 4117674..4118438 (-) 765 WP_005329211.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  GWI30_RS18965 (GWI30_19105) ampD 4118716..4119288 (-) 573 WP_161507563.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GWI30_RS18970 (GWI30_19110) - 4119434..4119895 (+) 462 WP_374393893.1 retropepsin-like aspartic protease family protein -
  GWI30_RS18975 (GWI30_19115) nadC 4119899..4120762 (+) 864 WP_161507565.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GWI30_RS18980 (GWI30_19120) - 4121075..4121530 (+) 456 WP_374393832.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GWI30_RS18985 (GWI30_19125) pilB 4121530..4123236 (+) 1707 WP_161507566.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  GWI30_RS18990 (GWI30_19130) pilC 4123366..4124607 (+) 1242 WP_161507567.1 type II secretion system F family protein Machinery gene
  GWI30_RS18995 (GWI30_19135) pilD 4124688..4125560 (+) 873 WP_161507568.1 prepilin peptidase Machinery gene
  GWI30_RS19000 (GWI30_19140) coaE 4125579..4126193 (+) 615 WP_161507569.1 dephospho-CoA kinase -
  GWI30_RS19005 (GWI30_19145) zapD 4126231..4126953 (+) 723 WP_161507570.1 cell division protein ZapD -
  GWI30_RS19010 (GWI30_19150) yacG 4126963..4127157 (+) 195 WP_042649030.1 DNA gyrase inhibitor YacG -
  GWI30_RS19015 (GWI30_19155) mutT 4127233..4127637 (-) 405 WP_161507571.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62639.06 Da        Isoelectric Point: 5.8224

>NTDB_id=418800 GWI30_RS18985 WP_161507566.1 4121530..4123236(+) (pilB) [Aeromonas media strain MC64]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEEGKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDDSVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKVVIRLLDSSVSRLNLDQLGFDERQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKVPEAVPEAELLEFGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITAN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=418800 GWI30_RS18985 WP_161507566.1 4121530..4123236(+) (pilB) [Aeromonas media strain MC64]
ATGACATCTAGCCTCAATAGCGGCCTGGCCACCAGCTTGGCCGCTTCCTCCCTCCTCAGTGAGCCCGACTCACAGCGCTA
TCTGAGCCAGGCCAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAAAACGACATCCTCGACAGCAAGGCTCTCG
CAGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTTGATCTCTCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAATTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACTCTCTATATCGCCATGTCAGA
CCCGACCAATGTGTCGGCCCTGGAGGACTTTGGCTTCAGCTTCGGCCTGCATACCGAAGCCTTGCTGGTCGAAGAGGGCA
AGCTGACCGCCGCCATCGGCAAGCTAATGGAAAGCGAGCAGGACACTCTGGGCATGGATCACATCGACGAAGCCGAAATC
TCGGAACTCGAGGTCTCCGATGAGAGTTCGCGGCTCGACGACAGTGTCAACACTGCCGACGACGATGCCCCCATCGTCAA
ATACATTCACAAGATCATGATGGACGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGC
TTAAAGGTAATGGCGCGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCCCGCAA
CCGGTCCATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAAAAGGTCGTCATTCGCCTGCTGGACTCGTCAG
TATCTCGCCTCAACCTAGACCAACTCGGCTTCGACGAACGCCAGAAGGCGCAATATCTGCATGCGCTCTCCAAGCCACAG
GGCATGATACTGGTCACGGGCCCCACGGGATCGGGCAAGACGGTGTCACTCTATACCGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCTCAGACCGGACACCTGGTGCTGTCAACCCTGCACACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GCCTCGCCCGCAAGTTGTGCGATCACTGCAAGGTGCCCGAGGCAGTGCCGGAAGCCGAATTGCTGGAGTTCGGTTTTACC
CAGCAACAGCTGGCCGCCGGTTTGCGGCTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGAGGATACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGAGAACATCGCCAAGCTGATCATGCAGGGAGCAAACTCGCTGCAGATCGCTG
CCATCGCCCAGAAGGAGGGTATGCGTACCCTGCGCATTTCCGGCCTGGAGAAGGCTCGTCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACGGCCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Acinetobacter baylyi ADP1

54.529

99.12

0.54
  pilB Legionella pneumophila strain ERS1305867

53.901

99.296

0.535
  pilB Vibrio cholerae strain A1552

52.837

99.296

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

49.378

99.12

0.489
  pilB Vibrio campbellii strain DS40M4

49.378

99.12

0.489
  pilF Neisseria gonorrhoeae MS11

46.384

99.824

0.463
  pilF Thermus thermophilus HB27

39.736

93.486

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

38.043

97.183

0.37

Multiple sequence alignment