Detailed information NTG0418802

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GWI30_RS18995
• Genome accession: NZ_CP047962
• Coordinates: 4124688..4125560 (+)
• Length: 290 a.a.
• NCBI ID: WP_161507568.1
• Uniprot ID: A0AAE6SLD1
• Organism: Aeromonas media strain MC64
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4119688..4130560

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GWI30_RS18975 (GWI30_19115)	nadC	4119899..4120762 (+)	864	WP_161507565.1	carboxylating nicotinate-nucleotide diphosphorylase	-
GWI30_RS18980 (GWI30_19120)	-	4121075..4121530 (+)	456	WP_374393832.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
GWI30_RS18985 (GWI30_19125)	pilB	4121530..4123236 (+)	1707	WP_161507566.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene
GWI30_RS18990 (GWI30_19130)	pilC	4123366..4124607 (+)	1242	WP_161507567.1	type II secretion system F family protein	Machinery gene
GWI30_RS18995 (GWI30_19135)	pilD	4124688..4125560 (+)	873	WP_161507568.1	prepilin peptidase	Machinery gene
GWI30_RS19000 (GWI30_19140)	coaE	4125579..4126193 (+)	615	WP_161507569.1	dephospho-CoA kinase	-
GWI30_RS19005 (GWI30_19145)	zapD	4126231..4126953 (+)	723	WP_161507570.1	cell division protein ZapD	-
GWI30_RS19010 (GWI30_19150)	yacG	4126963..4127157 (+)	195	WP_042649030.1	DNA gyrase inhibitor YacG	-
GWI30_RS19015 (GWI30_19155)	mutT	4127233..4127637 (-)	405	WP_161507571.1	8-oxo-dGTP diphosphatase MutT	-
GWI30_RS19020 (GWI30_19160)	secA	4127793..4130513 (-)	2721	WP_161507572.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32082.35 Da
• Isoelectric Point: 7.0918

>NTDB_id=418802 GWI30_RS18995 WP_161507568.1 4124688..4125560(+) (pilD) [Aeromonas media strain MC64]
MVPLIELAQGLPWLYLSLIFLFSLMIGSFLNVVIHRLPIMLEREWHADYRDYFGSEVATSPAEPYNLMVPRSRCPHCDHP
ISALENIPLLSWLWLKGRCRQCKAPISARYPLVELLTALLSVTVTATLTPGWGALAALALTWVLVALTFIDLDKMLLPDQ
LTLPLLWGGLLFNLAGGFVPLADAVIGAMAGYLVLWSLYWAFKLLTGKEGMGHGDFKLLAALGAWLGWQALPIVLLLSSL
VGAIIGIGLILLRNHHQGKPIPFGPYLAIAGWIALLWGDNITRWYLDIVL

Nucleotide

• Length: 873 bp

>NTDB_id=418802 GWI30_RS18995 WP_161507568.1 4124688..4125560(+) (pilD) [Aeromonas media strain MC64]
ATGGTGCCTCTGATCGAACTGGCCCAGGGCCTGCCCTGGCTCTATCTTTCCCTGATTTTTCTCTTCTCCCTGATGATTGG
CAGCTTCCTCAACGTGGTGATCCATCGCCTGCCCATCATGCTGGAGCGGGAGTGGCATGCAGACTACCGTGATTACTTCG
GCAGCGAGGTTGCCACTTCCCCCGCCGAACCTTACAACCTGATGGTACCGCGCTCTCGCTGCCCCCACTGCGATCACCCC
ATAAGCGCGCTGGAGAACATCCCCCTGCTGAGCTGGCTCTGGCTCAAGGGACGCTGCCGACAATGCAAGGCGCCAATTTC
GGCCCGCTATCCCCTGGTCGAGCTGCTGACCGCCCTGTTGTCGGTCACCGTTACGGCGACCCTCACTCCCGGCTGGGGAG
CCCTGGCAGCCCTCGCACTGACCTGGGTACTGGTGGCCCTCACCTTCATCGATCTGGACAAGATGCTGCTGCCGGACCAG
CTCACCCTGCCGCTGCTGTGGGGCGGTCTGTTGTTCAATCTGGCCGGCGGCTTTGTGCCGCTGGCCGATGCGGTGATCGG
CGCCATGGCTGGCTATCTGGTGCTCTGGAGTCTCTACTGGGCGTTCAAGCTGCTGACCGGCAAGGAGGGAATGGGTCATG
GCGACTTCAAGCTGCTGGCGGCACTCGGTGCCTGGCTGGGCTGGCAGGCGCTGCCCATAGTGCTGCTGCTCTCTTCACTG
GTCGGCGCCATCATTGGGATCGGACTTATCCTGCTGCGTAACCATCATCAGGGCAAACCCATCCCCTTTGGCCCCTACCT
CGCCATCGCGGGCTGGATAGCGCTGCTGTGGGGGGATAACATCACCCGCTGGTATCTGGACATCGTTCTCTAG

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-126)

• Type IV leader peptidase family (Peptidase_A24) (139-247)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	61.053	98.276	0.6
pilD	Vibrio campbellii strain DS40M4	55.709	99.655	0.555
pilD	Acinetobacter nosocomialis M2	52.518	95.862	0.503
pilD	Acinetobacter baumannii D1279779	52.158	95.862	0.5
pilD	Neisseria gonorrhoeae MS11	48.669	90.69	0.441