Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   E3S64_RS06200 Genome accession   NZ_CP047861
Coordinates   1215891..1216319 (-) Length   142 a.a.
NCBI ID   WP_000934385.1    Uniprot ID   A0A2K0CLZ8
Organism   Staphylococcus aureus strain UP_1322     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1175034..1225680 1215891..1216319 within 0


Gene organization within MGE regions


Location: 1175034..1225680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3S64_RS05895 (E3S64_05895) - 1175034..1175861 (-) 828 WP_000927632.1 sulfite exporter TauE/SafE family protein -
  E3S64_RS05900 (E3S64_05900) - 1175874..1176722 (-) 849 WP_000608129.1 DUF72 domain-containing protein -
  E3S64_RS05905 (E3S64_05905) - 1176770..1176853 (-) 84 WP_016170465.1 hypothetical protein -
  E3S64_RS05910 (E3S64_05910) - 1177064..1178131 (-) 1068 WP_000267236.1 NAD(P)H-dependent flavin oxidoreductase -
  E3S64_RS05915 (E3S64_05915) - 1178145..1179185 (-) 1041 WP_160199665.1 hemolysin family protein -
  E3S64_RS05920 (E3S64_05920) - 1179550..1179864 (+) 315 WP_000274029.1 hypothetical protein -
  E3S64_RS05925 (E3S64_05925) - 1179870..1180009 (-) 140 Protein_1141 hypothetical protein -
  E3S64_RS05930 (E3S64_05930) - 1180694..1180879 (-) 186 WP_001286805.1 hypothetical protein -
  E3S64_RS05935 (E3S64_05935) - 1180881..1180991 (-) 111 WP_000139423.1 hypothetical protein -
  E3S64_RS05940 (E3S64_05940) - 1181062..1181214 (-) 153 WP_001788502.1 hypothetical protein -
  E3S64_RS05945 (E3S64_05945) - 1181589..1182146 (+) 558 WP_001035620.1 DUF4888 domain-containing protein -
  E3S64_RS05950 (E3S64_05950) - 1182206..1183618 (-) 1413 WP_001141517.1 N-acetylmuramoyl-L-alanine amidase -
  E3S64_RS05955 (E3S64_05955) - 1183605..1183880 (-) 276 WP_000351119.1 phage holin -
  E3S64_RS05960 (E3S64_05960) - 1183936..1184331 (-) 396 WP_000398868.1 hypothetical protein -
  E3S64_RS05965 (E3S64_05965) - 1184337..1185575 (-) 1239 WP_160199659.1 BppU family phage baseplate upper protein -
  E3S64_RS05970 (E3S64_05970) - 1185588..1187486 (-) 1899 WP_000524022.1 glucosaminidase domain-containing protein -
  E3S64_RS05975 (E3S64_05975) - 1187623..1187922 (-) 300 WP_000466778.1 DUF2951 domain-containing protein -
  E3S64_RS05980 (E3S64_05980) - 1187962..1188135 (-) 174 WP_000782200.1 XkdX family protein -
  E3S64_RS05985 (E3S64_05985) - 1188139..1188516 (-) 378 WP_000705896.1 DUF2977 domain-containing protein -
  E3S64_RS05990 (E3S64_05990) - 1188516..1190339 (-) 1824 WP_000259619.1 phage baseplate upper protein -
  E3S64_RS05995 (E3S64_05995) - 1190339..1192249 (-) 1911 WP_000369017.1 hypothetical protein -
  E3S64_RS06000 (E3S64_06000) - 1192264..1194165 (-) 1902 WP_160199660.1 SGNH/GDSL hydrolase family protein -
  E3S64_RS06005 (E3S64_06005) - 1194174..1195121 (-) 948 WP_160199661.1 phage tail family protein -
  E3S64_RS06010 (E3S64_06010) - 1195134..1198598 (-) 3465 WP_103146790.1 hypothetical protein -
  E3S64_RS06015 (E3S64_06015) - 1198615..1198959 (-) 345 WP_000105584.1 hypothetical protein -
  E3S64_RS06020 (E3S64_06020) - 1198989..1199354 (-) 366 WP_001100163.1 tail assembly chaperone -
  E3S64_RS06025 (E3S64_06025) - 1199417..1199998 (-) 582 WP_000002578.1 phage major tail protein, TP901-1 family -
  E3S64_RS06030 (E3S64_06030) - 1200017..1200400 (-) 384 WP_000188643.1 hypothetical protein -
  E3S64_RS06035 (E3S64_06035) - 1200412..1200759 (-) 348 WP_001017815.1 HK97-gp10 family putative phage morphogenesis protein -
  E3S64_RS06040 (E3S64_06040) - 1200759..1201061 (-) 303 WP_001268314.1 hypothetical protein -
  E3S64_RS06045 (E3S64_06045) - 1201058..1201390 (-) 333 WP_000209972.1 phage head-tail connector protein -
  E3S64_RS06050 (E3S64_06050) - 1201399..1201686 (-) 288 WP_001114086.1 hypothetical protein -
  E3S64_RS06055 (E3S64_06055) - 1201708..1202682 (-) 975 WP_000438511.1 phage major capsid protein -
  E3S64_RS06060 (E3S64_06060) - 1202696..1203316 (-) 621 WP_000392151.1 DUF4355 domain-containing protein -
  E3S64_RS14505 - 1203310..1203397 (-) 88 Protein_1169 hypothetical protein -
  E3S64_RS06065 (E3S64_06065) - 1203425..1203595 (-) 171 WP_000072208.1 hypothetical protein -
  E3S64_RS06070 (E3S64_06070) - 1203668..1204663 (-) 996 WP_001133044.1 minor capsid protein -
  E3S64_RS06075 (E3S64_06075) - 1204670..1206208 (-) 1539 WP_000909970.1 phage portal protein -
  E3S64_RS06080 (E3S64_06080) - 1206219..1207514 (-) 1296 WP_000273011.1 PBSX family phage terminase large subunit -
  E3S64_RS06085 (E3S64_06085) - 1207517..1208011 (-) 495 WP_001038244.1 terminase small subunit -
  E3S64_RS06090 (E3S64_06090) - 1208199..1208621 (-) 423 WP_000162701.1 RinA family phage transcriptional activator -
  E3S64_RS06095 (E3S64_06095) - 1208690..1208791 (-) 102 Protein_1176 hypothetical protein -
  E3S64_RS06100 (E3S64_06100) rinB 1208792..1208965 (-) 174 WP_000595257.1 transcriptional activator RinB -
  E3S64_RS06105 (E3S64_06105) - 1208958..1209194 (-) 237 WP_000608278.1 hypothetical protein -
  E3S64_RS06110 (E3S64_06110) - 1209187..1209390 (-) 204 WP_001072795.1 hypothetical protein -
  E3S64_RS06115 (E3S64_06115) - 1209387..1209581 (-) 195 WP_000132921.1 hypothetical protein -
  E3S64_RS06120 (E3S64_06120) - 1209578..1209784 (-) 207 WP_000617154.1 DUF1381 domain-containing protein -
  E3S64_RS06125 (E3S64_06125) - 1209821..1210354 (-) 534 WP_000185647.1 dUTP diphosphatase -
  E3S64_RS06130 (E3S64_06130) - 1210347..1210517 (-) 171 WP_000714412.1 hypothetical protein -
  E3S64_RS06135 (E3S64_06135) - 1210504..1210758 (-) 255 WP_001065060.1 DUF1024 family protein -
  E3S64_RS06140 (E3S64_06140) - 1210751..1211122 (-) 372 WP_001557190.1 hypothetical protein -
  E3S64_RS06145 (E3S64_06145) - 1211131..1211373 (-) 243 WP_000131376.1 SAV1978 family virulence-associated passenger protein -
  E3S64_RS06150 (E3S64_06150) - 1211377..1211733 (-) 357 WP_000029376.1 SA1788 family PVL leukocidin-associated protein -
  E3S64_RS06155 (E3S64_06155) - 1211861..1212166 (-) 306 WP_000101252.1 hypothetical protein -
  E3S64_RS06160 (E3S64_06160) - 1212167..1212352 (-) 186 WP_001187243.1 DUF3113 family protein -
  E3S64_RS06165 (E3S64_06165) - 1212357..1212761 (-) 405 WP_000049794.1 DUF1064 domain-containing protein -
  E3S64_RS06170 (E3S64_06170) - 1212771..1212992 (-) 222 WP_001123695.1 DUF3269 family protein -
  E3S64_RS06175 (E3S64_06175) - 1213005..1213163 (-) 159 WP_000256589.1 hypothetical protein -
  E3S64_RS06180 (E3S64_06180) - 1213157..1213936 (-) 780 WP_000803062.1 ATP-binding protein -
  E3S64_RS06185 (E3S64_06185) - 1213946..1214717 (-) 772 Protein_1194 conserved phage C-terminal domain-containing protein -
  E3S64_RS06190 (E3S64_06190) - 1214783..1215064 (+) 282 WP_000414755.1 hypothetical protein -
  E3S64_RS14445 - 1215057..1215206 (-) 150 WP_001081076.1 hypothetical protein -
  E3S64_RS06195 (E3S64_06195) - 1215203..1215877 (-) 675 WP_001124438.1 putative HNHc nuclease -
  E3S64_RS06200 (E3S64_06200) ssbA 1215891..1216319 (-) 429 WP_000934385.1 single-stranded DNA-binding protein Machinery gene
  E3S64_RS06205 (E3S64_06205) - 1216319..1216942 (-) 624 WP_000139720.1 DUF1071 domain-containing protein -
  E3S64_RS06210 (E3S64_06210) - 1216935..1217156 (-) 222 WP_000815401.1 DUF2483 family protein -
  E3S64_RS06215 (E3S64_06215) - 1217166..1217426 (-) 261 WP_000291090.1 DUF1108 family protein -
  E3S64_RS06220 (E3S64_06220) - 1217431..1217733 (-) 303 WP_000165363.1 DUF2482 family protein -
  E3S64_RS06225 (E3S64_06225) - 1217827..1217988 (-) 162 WP_000066011.1 DUF1270 domain-containing protein -
  E3S64_RS06230 (E3S64_06230) - 1217981..1218202 (-) 222 WP_000977381.1 hypothetical protein -
  E3S64_RS06235 (E3S64_06235) - 1218216..1218665 (-) 450 WP_001094943.1 hypothetical protein -
  E3S64_RS06240 (E3S64_06240) tscA 1218705..1218929 (-) 225 WP_000187184.1 type II toxin-antitoxin system antitoxin TscA -
  E3S64_RS06245 (E3S64_06245) - 1218930..1219697 (-) 768 WP_001002757.1 phage antirepressor Ant -
  E3S64_RS06250 (E3S64_06250) - 1219697..1219891 (-) 195 WP_000108122.1 helix-turn-helix domain-containing protein -
  E3S64_RS06255 (E3S64_06255) - 1220154..1220486 (+) 333 WP_001055143.1 helix-turn-helix domain-containing protein -
  E3S64_RS06260 (E3S64_06260) - 1220503..1221177 (+) 675 WP_000775187.1 ImmA/IrrE family metallo-endopeptidase -
  E3S64_RS06265 (E3S64_06265) - 1221205..1221930 (+) 726 WP_000661437.1 PH domain-containing protein -
  E3S64_RS06270 (E3S64_06270) - 1221962..1222894 (+) 933 WP_000392186.1 hypothetical protein -
  E3S64_RS06275 (E3S64_06275) - 1222874..1223053 (-) 180 WP_000337826.1 hypothetical protein -
  E3S64_RS06280 (E3S64_06280) - 1223166..1224215 (+) 1050 WP_001145726.1 tyrosine-type recombinase/integrase -
  E3S64_RS06285 (E3S64_06285) sufB 1224283..1225680 (-) 1398 WP_001074405.1 Fe-S cluster assembly protein SufB -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 15872.58 Da        Isoelectric Point: 8.4825

>NTDB_id=418026 E3S64_RS06200 WP_000934385.1 1215891..1216319(-) (ssbA) [Staphylococcus aureus strain UP_1322]
MLNRAVLVGRLTKDPELRSAPNGVNVGTFTLAVNRTFTNAQGEREADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTR
SYENKVGQRVFVTEVVADSVQFLEPKNNNQQPNNNYHQQRQTQTGNNPFDNTTAITDDDLPF

Nucleotide


Download         Length: 429 bp        

>NTDB_id=418026 E3S64_RS06200 WP_000934385.1 1215891..1216319(-) (ssbA) [Staphylococcus aureus strain UP_1322]
ATGTTAAACAGAGCAGTATTAGTAGGACGCTTAACAAAAGACCCAGAATTAAGAAGCGCGCCAAATGGCGTAAATGTAGG
TACATTCACATTGGCAGTAAACAGAACATTCACGAATGCTCAAGGCGAGCGTGAAGCAGATTTTATAAACGTAGTAGTGT
TCAAGAAACAAGCTGAAAATGTTAAAAACTACCTTTCTAAAGGGTCGCTGGCAGGTGTAGACGGGCGACTACAAACACGT
AGCTACGAAAATAAAGTCGGGCAACGTGTATTTGTGACAGAAGTAGTAGCGGACAGTGTTCAATTCTTAGAACCGAAGAA
TAACAACCAACAACCAAACAACAATTATCATCAACAAAGACAAACTCAAACTGGTAATAATCCTTTTGATAATACCACTG
CGATTACTGATGATGACTTACCGTTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2K0CLZ8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

58.14

100

0.704

  ssb Latilactobacillus sakei subsp. sakei 23K

50.588

100

0.606

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

74.648

0.437

  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.958

100

0.423

  ssbA Streptococcus mutans UA159

40.845

100

0.408

  ssbB/cilA Streptococcus mitis NCTC 12261

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae Rx1

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae D39

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae R6

39.437

100

0.394

  ssbB/cilA Streptococcus mitis SK321

39.437

100

0.394


Multiple sequence alignment