Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbB   Type   Machinery gene
Locus tag   GU333_RS01105 Genome accession   NZ_CP047630
Coordinates   207243..207638 (+) Length   131 a.a.
NCBI ID   WP_031366343.1    Uniprot ID   A0A1K2H9Q1
Organism   Lactococcus raffinolactis strain Lr_18_12S     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 169596..228543 207243..207638 within 0


Gene organization within MGE regions


Location: 169596..228543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU333_RS00930 (GU333_00930) rfbB 169596..170645 (+) 1050 WP_167841758.1 dTDP-glucose 4,6-dehydratase -
  GU333_RS00935 (GU333_00935) rfbD 170642..171496 (+) 855 WP_167841759.1 dTDP-4-dehydrorhamnose reductase -
  GU333_RS00940 (GU333_00940) - 171610..172863 (+) 1254 WP_167841760.1 ISL3 family transposase -
  GU333_RS00945 (GU333_00945) - 173181..174386 (+) 1206 WP_167841761.1 glycosyltransferase family 1 protein -
  GU333_RS00950 (GU333_00950) - 174376..175317 (+) 942 WP_167841762.1 glycosyltransferase family 2 protein -
  GU333_RS00955 (GU333_00955) - 175314..176123 (+) 810 WP_167841763.1 ABC transporter permease -
  GU333_RS00960 (GU333_00960) - 176135..177316 (+) 1182 WP_167841764.1 ABC transporter ATP-binding protein -
  GU333_RS00965 (GU333_00965) - 177356..179389 (+) 2034 Protein_164 rhamnan synthesis F family protein -
  GU333_RS00970 (GU333_00970) - 179382..180734 (+) 1353 WP_167841766.1 DUF2142 domain-containing protein -
  GU333_RS00975 (GU333_00975) - 180738..181976 (+) 1239 WP_167841767.1 glycosyltransferase family 4 protein -
  GU333_RS00980 (GU333_00980) - 181973..182791 (+) 819 WP_167841768.1 glycosyltransferase -
  GU333_RS00985 (GU333_00985) - 182793..183521 (+) 729 WP_167841769.1 glycosyltransferase family 2 protein -
  GU333_RS00990 (GU333_00990) - 183521..183928 (+) 408 WP_167842505.1 DUF2304 domain-containing protein -
  GU333_RS00995 (GU333_00995) - 183929..184975 (+) 1047 WP_167841770.1 glycosyltransferase family A protein -
  GU333_RS01000 (GU333_01000) - 185055..186041 (+) 987 WP_167841771.1 glycosyltransferase family 2 protein -
  GU333_RS01005 (GU333_01005) - 186058..187053 (+) 996 WP_167841772.1 glycosyltransferase -
  GU333_RS01010 (GU333_01010) - 187050..187871 (+) 822 WP_167841773.1 LicD family protein -
  GU333_RS01015 (GU333_01015) - 187874..188596 (+) 723 WP_167841774.1 IspD/TarI family cytidylyltransferase -
  GU333_RS01020 (GU333_01020) - 188600..189652 (+) 1053 WP_167841775.1 NAD-dependent epimerase/dehydratase family protein -
  GU333_RS01025 (GU333_01025) - 189649..190596 (+) 948 WP_167841776.1 glycosyltransferase -
  GU333_RS01030 (GU333_01030) - 190609..191430 (+) 822 WP_167841777.1 glycosyltransferase family 2 protein -
  GU333_RS01035 (GU333_01035) - 191468..192415 (+) 948 WP_167841778.1 glycosyltransferase family 2 protein -
  GU333_RS01040 (GU333_01040) - 192455..193882 (+) 1428 WP_167841779.1 flippase -
  GU333_RS01045 (GU333_01045) - 193908..195602 (+) 1695 WP_167841780.1 hypothetical protein -
  GU333_RS01050 (GU333_01050) - 195612..197018 (+) 1407 WP_167841781.1 DUF2142 domain-containing protein -
  GU333_RS01055 (GU333_01055) glf 197096..198226 (+) 1131 WP_167841782.1 UDP-galactopyranose mutase -
  GU333_RS01060 (GU333_01060) - 198455..200374 (+) 1920 WP_167841783.1 Ig-like domain-containing protein -
  GU333_RS01065 (GU333_01065) - 200392..200766 (+) 375 WP_167841784.1 hypothetical protein -
  GU333_RS01070 (GU333_01070) - 200889..202262 (+) 1374 WP_167841785.1 GBS Bsp-like repeat-containing protein -
  GU333_RS01075 (GU333_01075) - 202280..203815 (+) 1536 WP_167841786.1 glucosaminidase domain-containing protein -
  GU333_RS01080 (GU333_01080) - 204220..204612 (+) 393 WP_167841787.1 HU family DNA-binding protein -
  GU333_RS01085 (GU333_01085) pepA 204671..205756 (-) 1086 WP_167841788.1 glutamyl aminopeptidase -
  GU333_RS01090 (GU333_01090) - 205896..206210 (+) 315 WP_031366346.1 hypothetical protein -
  GU333_RS01095 (GU333_01095) - 206212..206529 (+) 318 WP_167841789.1 thioredoxin family protein -
  GU333_RS01100 (GU333_01100) ytpR 206561..207184 (+) 624 WP_167841790.1 YtpR family tRNA-binding protein -
  GU333_RS01105 (GU333_01105) ssbB 207243..207638 (+) 396 WP_031366343.1 single-stranded DNA-binding protein Machinery gene
  GU333_RS01110 (GU333_01110) groES 207844..208128 (+) 285 WP_167841791.1 co-chaperone GroES -
  GU333_RS01115 (GU333_01115) groL 208187..209818 (+) 1632 WP_167841792.1 chaperonin GroEL -
  GU333_RS01120 (GU333_01120) - 209923..210444 (-) 522 WP_167838359.1 hypothetical protein -
  GU333_RS01125 (GU333_01125) - 210775..212067 (+) 1293 WP_138492029.1 adenylosuccinate synthase -
  GU333_RS01130 (GU333_01130) - 212191..214722 (+) 2532 WP_167841793.1 M1 family metallopeptidase -
  GU333_RS01135 (GU333_01135) - 214897..215100 (+) 204 WP_167841794.1 hypothetical protein -
  GU333_RS01140 (GU333_01140) - 215141..216610 (+) 1470 WP_138492032.1 SPFH domain-containing protein -
  GU333_RS01145 (GU333_01145) - 216567..216971 (-) 405 WP_167841795.1 hypothetical protein -
  GU333_RS01150 (GU333_01150) rplU 217010..217321 (+) 312 WP_061773673.1 50S ribosomal protein L21 -
  GU333_RS01155 (GU333_01155) - 217339..217671 (+) 333 WP_061773674.1 ribosomal-processing cysteine protease Prp -
  GU333_RS01160 (GU333_01160) rpmA 217835..218125 (+) 291 WP_003137946.1 50S ribosomal protein L27 -
  GU333_RS01165 (GU333_01165) pepT 218255..219487 (+) 1233 WP_167841796.1 peptidase T -
  GU333_RS01170 (GU333_01170) - 219551..219985 (-) 435 WP_167838363.1 universal stress protein -
  GU333_RS01175 (GU333_01175) - 220235..220531 (+) 297 WP_061773676.1 hypothetical protein -
  GU333_RS01180 (GU333_01180) - 220669..222720 (+) 2052 WP_167842506.1 sodium:proton antiporter -
  GU333_RS01185 (GU333_01185) - 222770..223912 (+) 1143 WP_167838364.1 cysteine desulfurase family protein -
  GU333_RS01190 (GU333_01190) thiI 223952..225178 (+) 1227 WP_167841797.1 tRNA uracil 4-sulfurtransferase ThiI -
  GU333_RS01195 (GU333_01195) - 225197..226606 (+) 1410 WP_096039933.1 MFS transporter -
  GU333_RS01200 (GU333_01200) - 227290..228543 (-) 1254 WP_167841760.1 ISL3 family transposase -

Sequence


Protein


Download         Length: 131 a.a.        Molecular weight: 14729.89 Da        Isoelectric Point: 5.9329

>NTDB_id=414717 GU333_RS01105 WP_031366343.1 207243..207638(+) (ssbB) [Lactococcus raffinolactis strain Lr_18_12S]
MLNKSLLIGRLTAQPEVKKTPTDKSVLRVTLAVSRQFKTADGQREADFLTIVIWGKPAELFASYAKKGSLVSIEGELRTR
RYEDKTGVTHYVTEVLCQQFNLLESRAAVALRENQVDDVSDVMLEAEELPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=414717 GU333_RS01105 WP_031366343.1 207243..207638(+) (ssbB) [Lactococcus raffinolactis strain Lr_18_12S]
ATGTTAAATAAGAGTTTATTAATTGGTCGTTTGACCGCACAGCCGGAAGTGAAAAAAACGCCGACGGATAAATCCGTCTT
GCGCGTGACCTTAGCGGTTAGTCGGCAGTTTAAGACTGCTGACGGCCAACGCGAGGCAGATTTTTTGACCATTGTCATCT
GGGGGAAACCAGCAGAGTTGTTCGCATCTTATGCTAAAAAAGGGAGTCTGGTCTCAATCGAGGGAGAACTACGGACACGA
CGGTATGAAGACAAGACGGGTGTGACTCACTATGTGACGGAAGTCTTATGCCAGCAGTTTAATTTACTAGAAAGTCGCGC
TGCCGTTGCTTTAAGAGAAAATCAAGTTGATGATGTCTCAGACGTGATGTTAGAGGCTGAAGAATTACCTTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A1K2H9Q1

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Streptococcus sobrinus strain NIDR 6715-7

64.394

100

0.649

  ssbA Streptococcus mutans UA159

62.879

100

0.634

  ssbB/cilA Streptococcus mitis NCTC 12261

59.848

100

0.603

  ssbB/cilA Streptococcus pneumoniae TIGR4

59.848

100

0.603

  ssbB/cilA Streptococcus pneumoniae Rx1

59.091

100

0.595

  ssbB/cilA Streptococcus pneumoniae D39

59.091

100

0.595

  ssbB/cilA Streptococcus pneumoniae R6

59.091

100

0.595

  ssbB/cilA Streptococcus mitis SK321

59.091

100

0.595

  ssbB Lactococcus lactis subsp. cremoris KW2

60

87.786

0.527

  ssb Latilactobacillus sakei subsp. sakei 23K

47.788

86.26

0.412

  ssbA Bacillus subtilis subsp. subtilis str. 168

45.283

80.916

0.366


Multiple sequence alignment