Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbB   Type   Machinery gene
Locus tag   GU336_RS02175 Genome accession   NZ_CP047616
Coordinates   405027..405422 (+) Length   131 a.a.
NCBI ID   WP_031366343.1    Uniprot ID   A0A1K2H9Q1
Organism   Lactococcus raffinolactis strain Lr_19_5     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 389846..409850 405027..405422 within 0


Gene organization within MGE regions


Location: 389846..409850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GU336_RS02110 (GU336_02110) - 389846..391159 (+) 1314 WP_167838348.1 glycosyltransferase -
  GU336_RS02115 (GU336_02115) - 391239..392168 (+) 930 WP_167838349.1 glycosyltransferase family 2 protein -
  GU336_RS02120 (GU336_02120) - 392241..393749 (+) 1509 WP_167838350.1 hypothetical protein -
  GU336_RS02125 (GU336_02125) - 393766..394173 (+) 408 WP_167838351.1 hypothetical protein -
  GU336_RS02130 (GU336_02130) - 394526..396007 (+) 1482 WP_167838352.1 CHAP domain-containing protein -
  GU336_RS02135 (GU336_02135) - 396191..397141 (-) 951 WP_167838195.1 IS30 family transposase -
  GU336_RS02140 (GU336_02140) - 397329..399071 (+) 1743 WP_167838353.1 serine hydrolase -
  GU336_RS02145 (GU336_02145) - 399213..400163 (-) 951 WP_167838195.1 IS30 family transposase -
  GU336_RS02150 (GU336_02150) - 400430..402400 (+) 1971 WP_167838354.1 glucosaminidase domain-containing protein -
  GU336_RS02155 (GU336_02155) pepA 402458..403543 (-) 1086 WP_167838355.1 glutamyl aminopeptidase -
  GU336_RS02160 (GU336_02160) - 403682..403996 (+) 315 WP_167838356.1 hypothetical protein -
  GU336_RS02165 (GU336_02165) - 403998..404315 (+) 318 WP_167838357.1 thioredoxin family protein -
  GU336_RS02170 (GU336_02170) ytpR 404345..404968 (+) 624 WP_031366344.1 YtpR family tRNA-binding protein -
  GU336_RS02175 (GU336_02175) ssbB 405027..405422 (+) 396 WP_031366343.1 single-stranded DNA-binding protein Machinery gene
  GU336_RS02180 (GU336_02180) groES 405628..405912 (+) 285 WP_003138237.1 co-chaperone GroES -
  GU336_RS02185 (GU336_02185) groL 405971..407602 (+) 1632 WP_167838358.1 chaperonin GroEL -
  GU336_RS02190 (GU336_02190) - 407707..408228 (-) 522 WP_167838359.1 hypothetical protein -
  GU336_RS02195 (GU336_02195) - 408558..409850 (+) 1293 WP_138492029.1 adenylosuccinate synthase -

Sequence


Protein


Download         Length: 131 a.a.        Molecular weight: 14729.89 Da        Isoelectric Point: 5.9329

>NTDB_id=414632 GU336_RS02175 WP_031366343.1 405027..405422(+) (ssbB) [Lactococcus raffinolactis strain Lr_19_5]
MLNKSLLIGRLTAQPEVKKTPTDKSVLRVTLAVSRQFKTADGQREADFLTIVIWGKPAELFASYAKKGSLVSIEGELRTR
RYEDKTGVTHYVTEVLCQQFNLLESRAAVALRENQVDDVSDVMLEAEELPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=414632 GU336_RS02175 WP_031366343.1 405027..405422(+) (ssbB) [Lactococcus raffinolactis strain Lr_19_5]
ATGTTAAATAAGAGTTTATTAATTGGTCGTTTGACCGCACAGCCGGAAGTGAAAAAAACGCCGACGGATAAATCTGTTTT
GCGCGTGACCTTAGCGGTTAGTCGGCAGTTTAAAACTGCTGACGGCCAACGCGAGGCGGATTTCTTGACCATTGTCATCT
GGGGGAAACCAGCAGAGTTGTTCGCATCATATGCTAAAAAAGGGAGTCTGGTCTCAATTGAGGGAGAACTACGGACACGA
CGGTATGAAGACAAGACGGGTGTGACCCACTATGTGACGGAAGTCTTATGCCAGCAGTTTAATTTACTAGAAAGTCGCGC
TGCCGTTGCTTTAAGAGAAAATCAAGTTGATGATGTCTCAGACGTGATGTTAGAGGCTGAAGAATTACCATTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A1K2H9Q1

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Streptococcus sobrinus strain NIDR 6715-7

64.394

100

0.649

  ssbA Streptococcus mutans UA159

62.879

100

0.634

  ssbB/cilA Streptococcus mitis NCTC 12261

59.848

100

0.603

  ssbB/cilA Streptococcus pneumoniae TIGR4

59.848

100

0.603

  ssbB/cilA Streptococcus pneumoniae Rx1

59.091

100

0.595

  ssbB/cilA Streptococcus pneumoniae D39

59.091

100

0.595

  ssbB/cilA Streptococcus pneumoniae R6

59.091

100

0.595

  ssbB/cilA Streptococcus mitis SK321

59.091

100

0.595

  ssbB Lactococcus lactis subsp. cremoris KW2

60

87.786

0.527

  ssb Latilactobacillus sakei subsp. sakei 23K

47.788

86.26

0.412

  ssbA Bacillus subtilis subsp. subtilis str. 168

45.283

80.916

0.366


Multiple sequence alignment