Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GRX76_RS08235 Genome accession   NZ_CP047569
Coordinates   2011654..2012877 (-) Length   407 a.a.
NCBI ID   WP_160152867.1    Uniprot ID   -
Organism   Microbulbifer sp. ALW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2006654..2017877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRX76_RS08205 - 2007165..2008622 (+) 1458 WP_160152862.1 NAD-dependent succinate-semialdehyde dehydrogenase -
  GRX76_RS08210 tsaA 2008651..2009367 (+) 717 WP_160152863.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  GRX76_RS08215 - 2009454..2009906 (+) 453 WP_160154887.1 protein disulfide oxidoreductase -
  GRX76_RS08220 yacG 2009903..2010109 (-) 207 WP_160152864.1 DNA gyrase inhibitor YacG -
  GRX76_RS08225 coaE 2010152..2010766 (-) 615 WP_160152865.1 dephospho-CoA kinase -
  GRX76_RS08230 - 2010768..2011652 (-) 885 WP_160152866.1 A24 family peptidase -
  GRX76_RS08235 pilC 2011654..2012877 (-) 1224 WP_160152867.1 type II secretion system F family protein Machinery gene
  GRX76_RS08240 pilB 2012928..2014652 (-) 1725 WP_160152868.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GRX76_RS19430 - 2015098..2015514 (+) 417 WP_305075899.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GRX76_RS08250 - 2016108..2016779 (+) 672 WP_160152870.1 hypothetical protein -
  GRX76_RS08255 - 2016962..2017234 (+) 273 WP_201276943.1 YdcH family protein -
  GRX76_RS08260 - 2017292..2017678 (-) 387 WP_160152871.1 DUF2750 domain-containing protein -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 43684.41 Da        Isoelectric Point: 9.8824

>NTDB_id=414216 GRX76_RS08235 WP_160152867.1 2011654..2012877(-) (pilC) [Microbulbifer sp. ALW1]
MANAAASAVATTYIYKGVDAKGNKVEGEASGTSPALVKAHLRRKGIIANRVQKKSAPLFGGKKKIKAGDIALFTRQLATM
TKAGVPLVQSFEIVADGSDNASLRKLILEIRDEVASGTSFAEALRKHPLYFDELFCNLVDSGEQSGALETMLGRIATYKE
KTESLKKKIKKAMTYPIAVVIVAVVVTAILLVKVVPTFAETFAGFGAELPAFTLFVLGISDWMQANWLIAFIGIVIGIGG
TLEIKKRNKKVAHFFDRLALKIPVLGQITYNSIAARYARTLSTTFAAGVPLIDALRSVAGATGNVIYQEATFRIRDTVAT
GVPLNTSMSTSKLFPPMLIQMTAIGEETGALDEMLSKAADFYEEAVDNMVDNLTALLEPMIMAVLGILVGGLLIAMYLPI
FQLGKVI

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=414216 GRX76_RS08235 WP_160152867.1 2011654..2012877(-) (pilC) [Microbulbifer sp. ALW1]
ATGGCCAATGCCGCCGCCAGTGCAGTAGCAACCACCTATATATATAAAGGCGTGGATGCCAAGGGCAACAAGGTCGAAGG
AGAGGCAAGCGGCACCAGTCCCGCACTGGTGAAAGCCCATCTCCGTCGCAAGGGTATTATTGCCAATCGAGTGCAGAAAA
AATCCGCCCCTCTGTTTGGCGGTAAAAAGAAAATTAAAGCTGGTGATATCGCGCTGTTTACCCGTCAGTTGGCAACTATG
ACCAAGGCTGGTGTACCCCTAGTTCAGAGCTTTGAAATCGTCGCCGATGGTAGCGATAACGCAAGCTTAAGAAAATTAAT
TCTTGAAATTAGGGATGAGGTAGCTTCCGGCACCAGCTTCGCCGAAGCATTGCGTAAACATCCACTCTATTTTGATGAGC
TGTTCTGCAACTTGGTGGATTCTGGTGAGCAATCGGGTGCTCTCGAGACCATGCTGGGAAGGATTGCCACCTACAAAGAA
AAAACGGAGTCATTGAAGAAGAAAATCAAGAAAGCAATGACCTACCCTATTGCCGTAGTCATCGTTGCTGTTGTTGTAAC
TGCTATTTTGCTCGTTAAGGTGGTACCCACTTTTGCTGAAACCTTTGCCGGCTTTGGTGCAGAGCTCCCTGCATTTACAC
TGTTCGTGCTCGGAATTTCTGACTGGATGCAAGCCAACTGGCTCATAGCCTTTATTGGAATCGTTATCGGCATTGGCGGC
ACTCTTGAAATTAAAAAGCGTAACAAGAAAGTGGCACACTTTTTCGACCGACTTGCACTGAAAATTCCAGTGCTCGGCCA
GATCACTTACAACTCAATTGCTGCACGTTATGCCCGCACACTCTCAACAACTTTTGCAGCAGGTGTCCCCCTGATTGATG
CACTTAGATCCGTTGCTGGCGCCACAGGTAATGTAATTTATCAGGAAGCAACCTTCAGAATTCGTGACACTGTTGCTACT
GGCGTGCCGCTCAATACTTCCATGAGCACATCTAAGCTTTTCCCACCCATGTTAATCCAGATGACCGCCATCGGTGAAGA
AACCGGTGCTCTGGATGAAATGCTGAGCAAGGCCGCAGACTTTTACGAAGAAGCTGTGGACAATATGGTTGACAATCTTA
CTGCCCTCCTTGAGCCCATGATCATGGCTGTATTGGGAATTCTAGTAGGTGGCCTGTTGATCGCCATGTACCTCCCCATC
TTCCAGCTCGGCAAAGTTATCTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(73-196)

Type II secretion system (T2SS), protein F (T2SSF)

(278-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

63.98

97.543

0.624
  pilC Acinetobacter baumannii D1279779

58.794

97.789

0.575
  pilC Acinetobacter baylyi ADP1

56.897

99.754

0.568
  pilC Legionella pneumophila strain ERS1305867

55.668

97.543

0.543
  pilG Neisseria gonorrhoeae MS11

43.939

97.297

0.428
  pilG Neisseria meningitidis 44/76-A

43.687

97.297

0.425
  pilC Vibrio cholerae strain A1552

41.31

97.543

0.403
  pilC Vibrio campbellii strain DS40M4

39.098

98.034

0.383
  pilC Thermus thermophilus HB27

35.422

100

0.361

Multiple sequence alignment