Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GRX76_RS08240 Genome accession   NZ_CP047569
Coordinates   2012928..2014652 (-) Length   574 a.a.
NCBI ID   WP_160152868.1    Uniprot ID   -
Organism   Microbulbifer sp. ALW1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2007928..2019652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRX76_RS08210 tsaA 2008651..2009367 (+) 717 WP_160152863.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  GRX76_RS08215 - 2009454..2009906 (+) 453 WP_160154887.1 protein disulfide oxidoreductase -
  GRX76_RS08220 yacG 2009903..2010109 (-) 207 WP_160152864.1 DNA gyrase inhibitor YacG -
  GRX76_RS08225 coaE 2010152..2010766 (-) 615 WP_160152865.1 dephospho-CoA kinase -
  GRX76_RS08230 - 2010768..2011652 (-) 885 WP_160152866.1 A24 family peptidase -
  GRX76_RS08235 pilC 2011654..2012877 (-) 1224 WP_160152867.1 type II secretion system F family protein Machinery gene
  GRX76_RS08240 pilB 2012928..2014652 (-) 1725 WP_160152868.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GRX76_RS19430 - 2015098..2015514 (+) 417 WP_305075899.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GRX76_RS08250 - 2016108..2016779 (+) 672 WP_160152870.1 hypothetical protein -
  GRX76_RS08255 - 2016962..2017234 (+) 273 WP_201276943.1 YdcH family protein -
  GRX76_RS08260 - 2017292..2017678 (-) 387 WP_160152871.1 DUF2750 domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62868.52 Da        Isoelectric Point: 4.9633

>NTDB_id=414217 GRX76_RS08240 WP_160152868.1 2012928..2014652(-) (pilB) [Microbulbifer sp. ALW1]
MSSTNSPPLSGLAKRLVLDKVIDEATAASALEAAKRERQSFAQHLVESKLVKSRELANSAAVAFGSPLYDLGSYNFELLP
KDVADEKLISKHFALPLYKRGSRLFIAVADPTNLAALDDFNFNTGLNTEAVLVESDKLAKAIESYLSNSGDMGGGLEGLD
DDDLDNLDVDGGDTPDQDRDNEPGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKIYRVRMRTDGILHEVARPPIQLAG
RLSARLKVMSKMDISERRVPQDGRIKMKVSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEEEQKQIYLDA
LDQPQGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVSNKVGLNFAEALRSFLRQDPDIVMV
GEIRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCNECKKPAQLPDEVLQ
DEGFDTVTIPKSEWQIFQPVGCEHCTKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADQARKEGFNNLRVSALRKVVM
GVTSLEEANRVTND

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=414217 GRX76_RS08240 WP_160152868.1 2012928..2014652(-) (pilB) [Microbulbifer sp. ALW1]
ATGAGCTCCACAAACAGCCCCCCCCTGAGCGGTCTGGCCAAGCGGCTGGTGCTCGACAAAGTAATCGATGAAGCCACAGC
AGCCTCAGCACTAGAGGCTGCAAAACGTGAACGCCAGAGTTTTGCCCAACACCTGGTGGAATCCAAACTGGTCAAAAGCA
GAGAACTGGCCAACAGTGCGGCAGTGGCATTTGGCAGCCCTCTATATGACCTAGGCAGTTACAACTTCGAACTGCTTCCC
AAAGATGTTGCCGATGAAAAATTGATCAGCAAGCATTTTGCTCTTCCCCTCTACAAACGGGGCAGTCGCCTTTTTATTGC
GGTGGCGGATCCAACCAACCTCGCGGCACTGGACGATTTCAATTTCAATACCGGCTTGAACACTGAAGCGGTATTGGTAG
AGTCAGATAAACTAGCCAAAGCGATTGAAAGCTATCTCTCCAATAGCGGCGATATGGGTGGCGGTCTAGAAGGGCTTGAT
GACGACGATCTTGACAATCTAGATGTGGACGGGGGGGATACACCCGACCAGGATCGAGATAACGAGCCTGGAGGAGACGA
AGCCCCTGTTGTACGTTTTGTGAACAAGGTGCTCTTGGACGCGATACGTACCGGTGCCTCCGATATTCACTTCGAACCCT
ATGAAAAGATCTATCGGGTACGCATGCGTACGGATGGTATTTTGCACGAGGTAGCGCGCCCTCCAATCCAGTTAGCCGGC
CGCCTTTCAGCCCGACTCAAGGTAATGTCCAAAATGGACATCTCCGAACGGCGAGTACCCCAGGATGGGCGGATCAAGAT
GAAGGTCTCCAAGACCAAGGCCATCGACTTCCGGGTGAATAGCCTGCCTACCCTGTGGGGTGAAAAAATCGTACTGCGGA
TTCTTGATCCCTCTTCAGCCAAACTGGGCATCGATGCCCTCGGCTACGAAGAAGAACAGAAACAGATTTACCTGGATGCG
CTAGATCAGCCCCAAGGTATGATTCTGGTAACTGGACCTACAGGCTCCGGTAAGACCGTATCCCTGTATACCGGCCTGAA
TATTCTCAACACCCCGGAGCGCAACATTTCTACTGCGGAAGATCCGGTGGAAATTAACCTGGAGGGGATCAACCAGGTCA
ATGTATCCAACAAGGTAGGTTTGAATTTCGCTGAGGCACTGCGCTCCTTCCTGCGTCAGGATCCGGATATCGTGATGGTG
GGGGAAATCCGGGATCTGGAAACTGCCGAGATCGCGATCAAGGCGGCGCAGACGGGTCACTTGGTACTATCGACCCTGCA
TACCAACTCTGCACCGGAAACATTGACCCGCCTGATGAATATGGGGGTTCCCACCTTCAATATCGCCACCTCGGTTAGTG
TGATTATCGCCCAGCGCCTGGCGCGACGCCTTTGTAACGAGTGTAAAAAGCCCGCACAGCTTCCAGACGAAGTATTGCAA
GATGAAGGATTTGATACGGTAACCATCCCAAAAAGCGAATGGCAGATATTCCAGCCAGTGGGCTGTGAGCACTGTACCAA
AGGCTACAAGGGACGCGTGGGTGTGTATGAAGTGGTTCGCATCACTGATGGAATTTCGCGCATTATAATGGAGGGCGGTA
ATTCAATTCAGATCGCCGACCAAGCACGCAAGGAAGGTTTCAATAACTTGCGCGTCTCCGCATTGCGGAAAGTGGTGATG
GGGGTCACCAGCCTGGAAGAAGCTAACCGGGTTACTAACGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.228

99.303

0.608
  pilB Acinetobacter baylyi ADP1

60

100

0.601
  pilB Legionella pneumophila strain ERS1305867

54.355

100

0.544
  pilB Vibrio campbellii strain DS40M4

55.128

95.122

0.524
  pilB Vibrio parahaemolyticus RIMD 2210633

54.945

95.122

0.523
  pilB Vibrio cholerae strain A1552

54.206

93.206

0.505
  pilF Neisseria gonorrhoeae MS11

47.803

99.129

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

37.702

100

0.406
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.028

98.955

0.376

Multiple sequence alignment