Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GTQ55_RS12880 Genome accession   NZ_CP047491
Coordinates   3006445..3007662 (+) Length   405 a.a.
NCBI ID   WP_161859105.1    Uniprot ID   A0A6P1TDA9
Organism   Microbulbifer hydrolyticus strain IRE-31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3001445..3012662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTQ55_RS12865 (GTQ55_12870) - 3002250..3003494 (-) 1245 WP_161859102.1 serine hydrolase domain-containing protein -
  GTQ55_RS12870 (GTQ55_12875) - 3003606..3003962 (-) 357 WP_420028978.1 pilin -
  GTQ55_RS18140 - 3003977..3004088 (-) 112 Protein_2532 pilin -
  GTQ55_RS12875 (GTQ55_12880) pilB 3004598..3006310 (+) 1713 WP_161859104.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GTQ55_RS12880 (GTQ55_12885) pilC 3006445..3007662 (+) 1218 WP_161859105.1 type II secretion system F family protein Machinery gene
  GTQ55_RS12885 (GTQ55_12890) pilD 3007674..3008558 (+) 885 WP_161859106.1 prepilin peptidase Machinery gene
  GTQ55_RS12890 (GTQ55_12895) coaE 3008562..3009173 (+) 612 WP_161859107.1 dephospho-CoA kinase -
  GTQ55_RS12895 (GTQ55_12900) yacG 3009188..3009409 (+) 222 WP_161859108.1 DNA gyrase inhibitor YacG -
  GTQ55_RS12900 (GTQ55_12905) - 3009471..3010301 (-) 831 WP_161859109.1 hypothetical protein -
  GTQ55_RS12905 (GTQ55_12910) - 3010539..3011813 (-) 1275 WP_161859110.1 hypothetical protein -
  GTQ55_RS12910 (GTQ55_12915) - 3011941..3012438 (-) 498 WP_161859111.1 protein disulfide oxidoreductase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43548.39 Da        Isoelectric Point: 9.5576

>NTDB_id=413782 GTQ55_RS12880 WP_161859105.1 3006445..3007662(+) (pilC) [Microbulbifer hydrolyticus strain IRE-31]
MAKAAAAVAYIYKGVDAKGNKVNGEITGTNPALIKAQLRRQGIIANRVQKKPKPLFGGGKKIKPGDIALFTRQLATMMRA
GVPLVQSFEIVADGSDNQSVKELILKIKEEVASGTAFADALRKHPLYFDDLFCNLVASGEQSGALETMLDRIATYKEKTE
SLKKKIKKAMTYPVMVIIVAVIVTAILLIKVVPQFASTFAGFGADLPAFTLFVLGISEWMQEYWLIAFVSLLVGVGGTLE
VKKRNKAVSEFFDRMILKIPVLGLITYNSIAARFSRTLATTFAAGVPLIDALKSVAGATGNVVYEQATLKIRDSVATGIP
LNAAMNQSGLYPNMLVQMAAIGEESGALDEMLGKAADFYEEAVDNLVDNLTTMLEPMIMAILGVLVGGLLVAMYLPIFNL
GNVIT

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=413782 GTQ55_RS12880 WP_161859105.1 3006445..3007662(+) (pilC) [Microbulbifer hydrolyticus strain IRE-31]
ATGGCCAAAGCTGCCGCTGCTGTTGCTTATATTTATAAGGGTGTCGATGCCAAAGGCAACAAGGTTAACGGGGAAATCAC
CGGAACCAATCCGGCGCTGATCAAAGCTCAGTTGCGCCGTCAGGGGATCATTGCCAACCGCGTACAAAAAAAACCCAAGC
CGCTTTTCGGTGGCGGTAAGAAAATCAAACCTGGTGATATAGCGTTATTTACCCGACAGCTGGCGACCATGATGAGAGCT
GGCGTGCCATTAGTACAGAGCTTCGAGATCGTTGCGGATGGCTCTGATAATCAGAGCGTCAAAGAACTCATCCTTAAAAT
CAAAGAGGAAGTAGCATCTGGTACTGCATTTGCTGACGCACTACGCAAGCATCCATTGTACTTTGATGACCTATTCTGCA
ATCTTGTAGCATCAGGTGAGCAGTCCGGTGCTCTTGAAACCATGCTTGACCGAATTGCGACCTACAAAGAAAAAACCGAA
TCTCTGAAGAAAAAAATCAAGAAAGCCATGACATACCCGGTCATGGTGATTATCGTAGCGGTCATTGTTACAGCCATTCT
TCTAATCAAAGTCGTCCCACAATTCGCGTCTACGTTTGCCGGCTTTGGCGCCGACCTCCCAGCATTTACTCTATTTGTAC
TTGGTATTTCCGAATGGATGCAAGAGTATTGGTTAATCGCTTTTGTATCACTGCTAGTTGGCGTAGGTGGAACTTTAGAG
GTAAAAAAGCGTAACAAAGCTGTTTCGGAATTTTTTGACCGAATGATCCTGAAAATTCCTGTCCTCGGTCTTATTACCTA
TAACTCTATTGCAGCACGCTTCTCCCGGACACTTGCAACAACCTTCGCAGCCGGCGTCCCTCTCATTGATGCCCTGAAGT
CGGTCGCCGGTGCAACCGGGAATGTAGTGTACGAACAAGCCACTTTGAAGATAAGAGACTCTGTGGCCACTGGCATTCCA
TTAAATGCAGCCATGAACCAATCTGGCTTGTACCCTAATATGTTGGTCCAGATGGCTGCCATCGGCGAAGAGTCAGGTGC
ACTGGATGAGATGTTAGGCAAAGCTGCAGATTTCTACGAAGAAGCCGTAGATAACCTGGTCGACAATTTAACCACCATGC
TTGAACCGATGATTATGGCCATTCTAGGTGTTTTAGTTGGTGGCTTACTGGTTGCAATGTACTTACCGATCTTTAACCTT
GGCAATGTAATCACTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(274-396)

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1TDA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.697

99.259

0.602
  pilC Acinetobacter baumannii D1279779

58.911

99.753

0.588
  pilC Acinetobacter baylyi ADP1

59.446

98.025

0.583
  pilC Legionella pneumophila strain ERS1305867

56.03

98.272

0.551
  pilG Neisseria gonorrhoeae MS11

44.862

98.519

0.442
  pilG Neisseria meningitidis 44/76-A

44.862

98.519

0.442
  pilC Vibrio cholerae strain A1552

41.919

97.778

0.41
  pilC Vibrio campbellii strain DS40M4

40.602

98.519

0.4
  pilC Thermus thermophilus HB27

36.207

100

0.363

Multiple sequence alignment