Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GTQ55_RS12875 Genome accession   NZ_CP047491
Coordinates   3004598..3006310 (+) Length   570 a.a.
NCBI ID   WP_161859104.1    Uniprot ID   A0A6P1TE31
Organism   Microbulbifer hydrolyticus strain IRE-31     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2999598..3011310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GTQ55_RS12860 (GTQ55_12865) - 3001330..3002199 (-) 870 WP_161859101.1 hypothetical protein -
  GTQ55_RS12865 (GTQ55_12870) - 3002250..3003494 (-) 1245 WP_161859102.1 serine hydrolase domain-containing protein -
  GTQ55_RS12870 (GTQ55_12875) - 3003606..3003962 (-) 357 WP_420028978.1 pilin -
  GTQ55_RS18140 - 3003977..3004088 (-) 112 Protein_2532 pilin -
  GTQ55_RS12875 (GTQ55_12880) pilB 3004598..3006310 (+) 1713 WP_161859104.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GTQ55_RS12880 (GTQ55_12885) pilC 3006445..3007662 (+) 1218 WP_161859105.1 type II secretion system F family protein Machinery gene
  GTQ55_RS12885 (GTQ55_12890) pilD 3007674..3008558 (+) 885 WP_161859106.1 prepilin peptidase Machinery gene
  GTQ55_RS12890 (GTQ55_12895) coaE 3008562..3009173 (+) 612 WP_161859107.1 dephospho-CoA kinase -
  GTQ55_RS12895 (GTQ55_12900) yacG 3009188..3009409 (+) 222 WP_161859108.1 DNA gyrase inhibitor YacG -
  GTQ55_RS12900 (GTQ55_12905) - 3009471..3010301 (-) 831 WP_161859109.1 hypothetical protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62199.95 Da        Isoelectric Point: 5.3488

>NTDB_id=413781 GTQ55_RS12875 WP_161859104.1 3004598..3006310(+) (pilB) [Microbulbifer hydrolyticus strain IRE-31]
MSSTPLSGLAKRLVSDNAIDEATAQSAIKAARREGQTFAQHVVEAKLIKSRELANIASSAFGSPVFDLSCYNFDLLPKDV
VAENLISKHFALPLYKRGNRLFVAVADPTNLAGLDEINFNTGLNTDAVLVEADKLGKAIESYLSNSGDISGGLEGLDDDD
LDGLDIDSAEASRDDDNEPGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKLYRVRLRTDGVLHEVARPPIQLATRISA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEENQKKIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGINQVNVSNKVGLNFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCGECKKPAKLPDEVLKEEGF
DTVTIPKSEWQIFQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADQARKEGFDNLRTSALRKVVMGVTS
LEEANRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=413781 GTQ55_RS12875 WP_161859104.1 3004598..3006310(+) (pilB) [Microbulbifer hydrolyticus strain IRE-31]
ATGAGCAGCACCCCACTGAGCGGTCTAGCCAAACGGCTGGTATCTGACAACGCCATCGATGAGGCTACGGCCCAGTCGGC
CATCAAGGCGGCCAGACGTGAAGGCCAGACCTTTGCCCAGCACGTAGTGGAGGCCAAGCTGATCAAAAGCAGAGAACTGG
CCAATATTGCATCATCGGCATTTGGCAGTCCTGTGTTTGACCTTTCTTGTTATAACTTTGACCTGCTTCCCAAGGATGTT
GTCGCTGAAAATTTGATCAGCAAACATTTTGCCCTCCCCCTTTATAAACGCGGTAACCGACTGTTTGTGGCCGTCGCAGA
CCCCACCAATCTGGCCGGGCTGGATGAGATCAATTTCAATACCGGTCTTAACACTGACGCCGTACTGGTGGAGGCGGATA
AGCTCGGCAAGGCCATTGAAAGCTATCTTTCCAACAGCGGTGATATCAGTGGTGGCTTAGAAGGCTTAGATGACGACGAT
CTGGATGGCCTAGATATAGATAGCGCTGAGGCCAGTCGCGACGATGACAACGAACCCGGGGGTGACGAAGCGCCGGTTGT
TCGCTTTGTGAACAAAGTGTTGCTGGATGCTATTCGTACAGGTGCATCCGATATTCACTTCGAACCCTATGAAAAGCTGT
ATAGAGTCCGCTTGCGTACCGACGGTGTGCTACATGAAGTGGCACGCCCCCCGATCCAGCTGGCTACCCGCATCTCTGCC
CGACTGAAAGTAATGTCGAAGATGGATATTTCAGAGCGTCGGGTACCCCAGGACGGTCGTATCAAGATGAAGCTGTCTAA
GACCAAGGCCATCGACTTCCGGGTAAATAGCCTGCCAACCCTGTGGGGCGAGAAAATCGTACTGCGGATACTAGACCCCT
CCTCGGCCAAACTAGGCATTGATGCTCTGGGTTATGAAGAAAACCAGAAGAAAATCTATATGGATGCTCTGGCCCAGCCG
CAGGGCATGATTCTGGTAACCGGTCCCACCGGCTCGGGTAAAACAGTATCTCTTTACACCGGCCTGAATATTCTCAATAC
CGCTGAGCGCAATATCTCTACAGCGGAAGACCCGGTAGAAATCAACCTGGAGGGGATCAATCAGGTAAACGTCTCCAATA
AGGTGGGGCTCAACTTCGCAGAGGCGCTGCGCTCCTTTCTGCGCCAGGACCCCGACATCGTCATGGTTGGGGAGATCCGG
GACCTGGAAACGGCAGAAATCGCCATCAAGGCGGCACAGACAGGCCACCTTGTGCTCTCTACCCTGCACACCAACTCCGC
CCCGGAAACCCTGACCCGCCTGATGAATATGGGGGTACCCACGTTTAACATTGCCACTTCCGTGAGCGTGATTATTGCCC
AGCGCCTTGCTCGCAGACTTTGTGGTGAATGTAAGAAGCCGGCCAAACTGCCGGATGAAGTACTTAAGGAAGAGGGTTTC
GACACGGTCACTATTCCCAAAAGCGAATGGCAAATATTCCAGCCTGTTGGTTGCGAGCACTGCTCAAAGGGATACAAAGG
GCGCGTGGGTGTGTATGAAGTGGTTCGCATCACTGATGGAATTTCACGCATTATAATGGAGGGTGGCAACTCTATTCAGA
TTGCCGATCAGGCCAGAAAGGAGGGTTTCGATAACCTGCGCACATCGGCTTTGCGCAAGGTGGTAATGGGGGTTACCAGC
CTTGAAGAAGCCAATCGAGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P1TE31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.633

99.825

0.605
  pilB Acinetobacter baylyi ADP1

58.947

100

0.589
  pilB Legionella pneumophila strain ERS1305867

54.482

99.825

0.544
  pilB Vibrio campbellii strain DS40M4

51.327

99.123

0.509
  pilB Vibrio cholerae strain A1552

50.261

100

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

52.747

95.789

0.505
  pilF Neisseria gonorrhoeae MS11

47.443

99.474

0.472
  pilB/pilB1 Synechocystis sp. PCC 6803

37.724

100

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.478

98.772

0.37

Multiple sequence alignment