Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PMA4326_RS04110 Genome accession   NZ_CP047260
Coordinates   882656..883873 (-) Length   405 a.a.
NCBI ID   WP_007251659.1    Uniprot ID   A0A8T8BXM8
Organism   Pseudomonas syringae pv. maculicola str. ES4326     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 877656..888873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMA4326_RS04085 (PMA4326_004080) tnpB 878495..878854 (+) 360 WP_007247726.1 IS66 family insertion sequence element accessory protein TnpB -
  PMA4326_RS04090 (PMA4326_004085) - 878871..880403 (+) 1533 WP_159372363.1 IS66 family transposase -
  PMA4326_RS04095 (PMA4326_004090) yacG 880956..881165 (-) 210 WP_007251656.1 DNA gyrase inhibitor YacG -
  PMA4326_RS04100 (PMA4326_004095) coaE 881162..881785 (-) 624 WP_007251657.1 dephospho-CoA kinase -
  PMA4326_RS04105 (PMA4326_004100) pilD 881782..882654 (-) 873 WP_007251658.1 A24 family peptidase Machinery gene
  PMA4326_RS04110 (PMA4326_004105) pilC 882656..883873 (-) 1218 WP_007251659.1 type II secretion system F family protein Machinery gene
  PMA4326_RS04115 (PMA4326_004110) pilB 883876..885570 (-) 1695 WP_007251660.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PMA4326_RS04120 (PMA4326_004115) - 885796..886185 (+) 390 WP_007251661.1 pilin -
  PMA4326_RS04125 (PMA4326_004120) - 886245..887657 (+) 1413 WP_042913806.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44199.17 Da        Isoelectric Point: 10.1002

>NTDB_id=411890 PMA4326_RS04110 WP_007251659.1 882656..883873(-) (pilC) [Pseudomonas syringae pv. maculicola str. ES4326]
MASKAVKVSIYTWEGVDKKGAKTGGELSGHNLALVKAQLRKQGINPTKVREKSVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVGSLKQEVSAGNSFATALRKKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPTAVLIVAFIVSGILLIKVVPQFQSVFAGFNAELPAFTLMVIALSEVVQQWWLAIVVLFFVTFFMLK
RAYKRSQKFRDRLDRFALKIPIIGPLIFKSCVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNQVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDAMLDKVATYYEEEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=411890 PMA4326_RS04110 WP_007251659.1 882656..883873(-) (pilC) [Pseudomonas syringae pv. maculicola str. ES4326]
ATGGCCAGCAAGGCAGTCAAAGTCAGCATTTACACTTGGGAAGGTGTCGACAAGAAAGGCGCAAAAACCGGCGGAGAACT
GAGCGGCCATAATCTGGCACTTGTGAAGGCCCAACTGCGTAAGCAAGGCATCAATCCGACCAAGGTACGCGAAAAGTCAG
TTTCTATTTTTGGCAAGGGCAAGAAGATCAAGCCACTGGACATTGCGTTCTTTTCTCGTCAGATGGCGACGATGATGAAG
GCTGGCGTGCCCTTGCTACAGTCATTCGACATCATCAGTGAGGGCGCAGAGAATCCAAATATGCGTGCGCTGGTCGGCTC
ACTGAAACAGGAAGTTTCAGCCGGTAACAGCTTCGCAACGGCCTTACGGAAGAAGCCTGAATACTTCGATGATTTGTTTT
GCAATCTGGTGGATGCCGGTGAGCAAGCAGGCGCCCTTGAAAGCCTGCTCGACCGAGTAGCAAGCTATAAGGAAAAGACT
GAAAAGCTCAAAGCCAAAATCAAGAAAGCCATGACCTATCCGACTGCCGTATTGATTGTGGCTTTTATTGTCTCAGGCAT
TTTGCTGATTAAAGTAGTACCGCAGTTTCAATCAGTTTTTGCCGGATTTAACGCCGAACTACCTGCATTTACTCTGATGG
TTATTGCGCTTTCGGAGGTTGTCCAACAGTGGTGGCTGGCAATTGTCGTACTGTTTTTTGTGACTTTTTTCATGCTCAAG
AGAGCCTACAAACGATCTCAAAAATTTCGTGACCGACTTGATCGTTTTGCATTGAAAATCCCCATTATCGGACCGCTTAT
TTTCAAATCCTGCGTCGCGCGATACGCACGCACTCTCGCTACCACGTTTGCGGCGGGTGTTCCACTGGTTGAGGCGCTGG
ACTCGGTCGCCGGAGCGACTGGCAATGTGGTGTTCCGGAACGCAGTCAATCAAGTCAAACAGGATGTATCTACAGGCATG
CAGCTGAATTTCTCGATGCGCTCGACAGGCGTTTTTCCTAGCCTCGCTATACAGATGACCGCGATTGGGGAAGAGTCCGG
CGCACTTGACGCGATGCTTGACAAGGTAGCGACGTATTACGAAGAAGAAGTCGACAACATGGTTGATAGCCTGACCAGCC
TGATGGAACCGATGATCATGGCGGTATTGGGTGTCATCGTCGGTGGTCTGGTCATTGCGATGTATCTGCCAATTTTCAAA
CTTGGAAGCGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.827

100

0.738
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

59.653

99.753

0.595
  pilC Legionella pneumophila strain ERS1305867

52.02

97.778

0.509
  pilG Neisseria gonorrhoeae MS11

42.432

99.506

0.422
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Vibrio cholerae strain A1552

41.235

100

0.412
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment