Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PMA4326_RS04115 Genome accession   NZ_CP047260
Coordinates   883876..885570 (-) Length   564 a.a.
NCBI ID   WP_007251660.1    Uniprot ID   A0A8T8BWX7
Organism   Pseudomonas syringae pv. maculicola str. ES4326     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 878876..890570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMA4326_RS04095 (PMA4326_004090) yacG 880956..881165 (-) 210 WP_007251656.1 DNA gyrase inhibitor YacG -
  PMA4326_RS04100 (PMA4326_004095) coaE 881162..881785 (-) 624 WP_007251657.1 dephospho-CoA kinase -
  PMA4326_RS04105 (PMA4326_004100) pilD 881782..882654 (-) 873 WP_007251658.1 A24 family peptidase Machinery gene
  PMA4326_RS04110 (PMA4326_004105) pilC 882656..883873 (-) 1218 WP_007251659.1 type II secretion system F family protein Machinery gene
  PMA4326_RS04115 (PMA4326_004110) pilB 883876..885570 (-) 1695 WP_007251660.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PMA4326_RS04120 (PMA4326_004115) - 885796..886185 (+) 390 WP_007251661.1 pilin -
  PMA4326_RS04125 (PMA4326_004120) - 886245..887657 (+) 1413 WP_042913806.1 O-antigen ligase family protein -
  PMA4326_RS04130 (PMA4326_004125) - 887830..889116 (+) 1287 WP_042913808.1 polysaccharide biosynthesis C-terminal domain-containing protein -
  PMA4326_RS04135 (PMA4326_004130) - 889138..889515 (-) 378 WP_007251664.1 helix-turn-helix domain-containing protein -
  PMA4326_RS04140 (PMA4326_004135) gstA 889670..890290 (+) 621 WP_007251665.1 glutathione transferase GstA -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62538.93 Da        Isoelectric Point: 6.3321

>NTDB_id=411891 PMA4326_RS04115 WP_007251660.1 883876..885570(-) (pilB) [Pseudomonas syringae pv. maculicola str. ES4326]
MTDVVLTGLAKQLVLAELLTETVAQQAYQQARRDKISLVSYLVLNKLVKSLTLAEMASDQFGVPFMDLACLDRESQPKGL
VSEKLVRQHHALPLWRRGNKLFIAISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDSGLGNLEDVDLGLD
IEPSSDKETSLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKLPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPHETLIKEGFPEAK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=411891 PMA4326_RS04115 WP_007251660.1 883876..885570(-) (pilB) [Pseudomonas syringae pv. maculicola str. ES4326]
ATGACTGATGTTGTCCTCACTGGTTTGGCAAAACAACTGGTTCTGGCCGAGCTACTTACTGAAACTGTGGCGCAGCAAGC
GTATCAACAGGCGCGCCGTGACAAGATTTCGTTGGTTAGCTATCTGGTTCTGAACAAACTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCTGACCAATTTGGTGTGCCGTTCATGGACCTTGCCTGCCTGGACAGGGAGAGTCAGCCAAAGGGACTG
GTAAGTGAAAAGCTGGTCAGACAGCACCATGCCCTCCCCCTGTGGCGACGTGGCAATAAACTATTCATCGCGATTTCGGA
CCCTACCAACCACCAGGCCGTGACTGATATTCAATTCAGCACGGGCCTGAATACTGAAGCGATTCTCGTCGAAGACGACA
AGCTGACCACAGCAATTGATCGCTTTTTTGACAGTGATAGCGGTCTTGGCAATCTGGAAGATGTCGATCTGGGCCTTGAC
ATCGAGCCCTCCAGTGACAAGGAAACCTCGCTTGCTACACAAAATGATGCCGATGACGCCCCAGTGGTGCGATTCGTCAA
CAAAATGCTGATGGACGCAATCCGGTTAGGATCATCCGATCTGCACTTCGAGCCCTATGAGAAAATTTTTCGCGTGCGCT
TGCGTACAGACGGCATCCTGCATGAAGTCGCCAGACCGCCCATTCATCTGGCAAACCGTATAGCTGCGCGCTTGAAAGTC
ATGGCCAGCCTCGATATATCGGAACGGCGCAAACCGCAGGACGGTCGGGTCAAGCTGCGGGTGTCCAAAACAAAAGCCAT
CGACTTCCGTATGAACACCTTGCCCACGTTATGGGGCGAGAAGATCGTGATGCGGATCCTCGACCCGACCAGCGCCCAAA
TGGGCATTGATGCTCTGGGCTACGAGCCAGAACAAAAGGCACTGTACCTGGAAGCGCTGAAACTGCCGCAAGGCATGATT
CTTGTAACTGGCCCTACAGGTTCCGGCAAGACCGTTTCCTTATATACCGGCCTGAATATCCTCAACACCGTGGACATCAA
CATTTCCACAGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAATCAAGTCAACGTCAACCCGCGCCAGGGATTGG
ACTTTTCTCAGGCACTACGCGCATTTCTGCGTCAAGATCCCGACGTAATCATGGTCGGTGAGATTCGAGACCTGGAAACC
GCTGAAATTGCCATCAAGGCATCTCAGACGGGTCATATGGTGCTATCAACCCTGCACACCAACAGCGCTGCGGAAACATT
GACGCGCCTGCACCACATGGGCGTCGCAGCATTCAATATTGCAACTGCGATCAACCTGATTATTGCGCAGCGACTCGCAC
GCAAATTATGCAGCCACTGCAAGAAGGAACTCGATATCCCTCATGAGACACTGATCAAAGAAGGCTTTCCGGAGGCTAAA
ATCGGTACATTCAAGATCTATGGCCCCGTAGGCTGCGAGCATTGCAATGGCGGCTACAGAGGCCGGGTCGGCATCTACGA
GGTGGTCAAGAAAACAGCAGAACTGGAGCGCATCATCATGGAAGAGGGCAACTCGCTGGAAATATCCAGGCAGATGCGTA
AGGACGGCTTCAATGACCTGCGCACCTCAGGCTTGTCAAAAGCCATGCAGGGCATAACCAGCCTTGAAGAAGTCAATCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.082

99.823

0.58
  pilB Acinetobacter baylyi ADP1

56.762

99.645

0.566
  pilB Legionella pneumophila strain ERS1305867

55.752

100

0.559
  pilB Vibrio cholerae strain A1552

51.246

99.645

0.511
  pilB Vibrio campbellii strain DS40M4

49.291

100

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

50.455

97.34

0.491
  pilF Neisseria gonorrhoeae MS11

48.663

99.468

0.484
  pilF Thermus thermophilus HB27

38.693

100

0.388

Multiple sequence alignment