Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   METME_RS02335 Genome accession   NC_015572
Coordinates   501377..502543 (+) Length   388 a.a.
NCBI ID   WP_013817185.1    Uniprot ID   G0A2A1
Organism   Methylomonas methanica MC09     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 496377..507543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  METME_RS02305 (Metme_0463) zapD 496825..497595 (+) 771 WP_013817179.1 cell division protein ZapD -
  METME_RS02310 (Metme_0464) - 497595..497798 (+) 204 WP_013817180.1 DNA gyrase inhibitor YacG -
  METME_RS02315 (Metme_0465) - 497808..498743 (-) 936 WP_013817181.1 Nudix family hydrolase -
  METME_RS02320 (Metme_0466) argJ 498744..499958 (-) 1215 WP_013817182.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  METME_RS02325 (Metme_0467) - 499979..500203 (-) 225 WP_013817183.1 sulfurtransferase TusA family protein -
  METME_RS02330 (Metme_0468) - 500200..501243 (-) 1044 WP_013817184.1 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase -
  METME_RS02335 (Metme_0469) pilU 501377..502543 (+) 1167 WP_013817185.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  METME_RS02340 (Metme_0470) fba 502559..503593 (-) 1035 WP_013817186.1 class II fructose-bisphosphate aldolase -
  METME_RS02345 (Metme_0471) - 504056..504529 (-) 474 WP_013817187.1 hypothetical protein -
  METME_RS02350 (Metme_0472) - 504794..505114 (-) 321 WP_013817188.1 NIF3 1 -
  METME_RS24575 (Metme_0473) - 505124..505276 (-) 153 WP_013817189.1 hypothetical protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 42880.98 Da        Isoelectric Point: 5.6361

>NTDB_id=40920 METME_RS02335 WP_013817185.1 501377..502543(+) (pilU) [Methylomonas methanica MC09]
MDFKDYLKILVQHDGSDLYLTAGAPPAAKFQGTLKPIENIKLTSERIKEIANNIMDADQRAAFEHVPEMNLAIAESGVGR
FRVNIFKQRNSYALVIRNIKVDIPNADVLGLPQILKDKIMEKRGLILFVGGTGSGKSTSLAALIDYRNSNSSGHIITIED
PIEYVHPHKKSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLQAFVSQRLVPTIEGKRVAAIEILLGTKLVSDLIQKGEVHAIKEAMEKSENIGMQTFDSHL
MKLYKSGVISLEEALRNSDSPNNLKLKINLSEGLGSATAPKAENGKSMLDSLALEEIAKSEDEEDEGH

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=40920 METME_RS02335 WP_013817185.1 501377..502543(+) (pilU) [Methylomonas methanica MC09]
ATGGACTTTAAAGATTATTTGAAAATACTGGTGCAACACGACGGCTCCGACCTTTATTTAACAGCAGGTGCGCCGCCGGC
GGCAAAGTTTCAAGGCACGCTAAAGCCGATAGAAAATATTAAGTTGACGAGCGAAAGGATAAAAGAGATCGCCAACAATA
TCATGGATGCCGATCAGCGGGCGGCGTTCGAGCATGTGCCTGAAATGAATCTGGCCATCGCCGAATCCGGCGTGGGCCGT
TTCAGGGTGAATATTTTCAAGCAGCGTAATAGCTACGCGCTGGTGATCCGCAATATCAAGGTCGATATTCCCAATGCCGA
CGTTTTGGGCTTGCCGCAGATATTGAAAGACAAGATTATGGAAAAACGCGGTTTGATCCTGTTTGTTGGTGGTACCGGTT
CAGGCAAGTCCACCTCGTTGGCGGCGTTGATCGATTATCGCAATAGCAACTCATCCGGCCATATCATCACTATTGAAGAT
CCCATCGAATATGTGCATCCGCATAAAAAATCATTGGTCAACCAACGTGAAGTCGGCGTGGATACGCTAAGTTACGAAGA
TGCATTGAAAAACACCCTGCGGCAGGCGCCGGATGTGATTTTAATTGGCGAAATCCGTAGCCAGGAAACCATGGAGCATG
CCCTGGCGTTTGCGGAAACCGGCCACTTATGTTTGTCCACGCTGCATGCCAACAATGCCAATCAGGCGCTGGACAGGATC
ATCAACTTTTTCCCGGAAGAGCGCCGTAACCAACTATTGTTGGATTTATCCCTAAATCTGCAGGCCTTTGTCTCGCAACG
TTTGGTGCCGACGATCGAGGGTAAGCGGGTGGCGGCGATTGAAATATTGCTGGGTACCAAGCTGGTCAGCGATTTGATTC
AGAAAGGCGAGGTGCATGCCATCAAGGAAGCCATGGAAAAATCGGAAAACATCGGCATGCAAACGTTCGACAGCCATTTG
ATGAAGTTGTACAAGAGCGGGGTGATTTCGCTGGAAGAAGCCTTGCGTAATTCGGATTCGCCCAACAATTTGAAATTGAA
GATCAATCTCAGCGAAGGATTGGGCTCGGCCACAGCACCAAAAGCCGAGAACGGCAAAAGTATGCTGGATAGTTTGGCGT
TGGAAGAAATTGCCAAAAGCGAAGATGAAGAGGACGAGGGTCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(48-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0A2A1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.851

96.907

0.541
  pilU Acinetobacter baylyi ADP1

54.237

91.237

0.495
  pilU Vibrio cholerae strain A1552

49.153

91.237

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.934

93.814

0.384
  pilT Pseudomonas aeruginosa PAK

41.279

88.66

0.366
  pilT Legionella pneumophila strain ERS1305867

43.03

85.052

0.366
  pilT Legionella pneumophila strain Lp02

43.03

85.052

0.366
  pilT Acinetobacter nosocomialis M2

40.988

88.66

0.363
  pilT Acinetobacter baumannii D1279779

40.988

88.66

0.363
  pilT Acinetobacter baumannii strain A118

40.988

88.66

0.363

Multiple sequence alignment