Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   GO175_RS02165 Genome accession   NZ_CP046855
Coordinates   353022..353453 (+) Length   143 a.a.
NCBI ID   WP_154184015.1    Uniprot ID   -
Organism   Vibrio fluvialis strain F8658     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 348022..358453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO175_RS02140 dsbC 348843..349607 (-) 765 WP_158147897.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  GO175_RS02145 xerD 349624..350532 (-) 909 WP_158147898.1 site-specific tyrosine recombinase XerD -
  GO175_RS02150 fldB 350673..351194 (+) 522 WP_020430452.1 flavodoxin FldB -
  GO175_RS02155 ampD 351199..351750 (-) 552 WP_158147899.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GO175_RS02160 nadC 351903..352790 (+) 888 WP_061056143.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GO175_RS02165 pilA 353022..353453 (+) 432 WP_154184015.1 pilin Machinery gene
  GO175_RS02170 pilB 353460..355147 (+) 1688 Protein_309 type IV-A pilus assembly ATPase PilB -
  GO175_RS02175 pilC 355183..356409 (+) 1227 WP_020332199.1 type II secretion system F family protein Machinery gene
  GO175_RS02180 pilD 356461..357330 (+) 870 WP_158147900.1 A24 family peptidase Machinery gene
  GO175_RS02185 coaE 357333..357941 (+) 609 WP_158147901.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14768.80 Da        Isoelectric Point: 8.4687

>NTDB_id=408930 GO175_RS02165 WP_154184015.1 353022..353453(+) (pilA) [Vibrio fluvialis strain F8658]
MNANQRKQQQGFTLIELMIVVAIIGVLSAIAVPAYKNYVAKSEAASALATLKSLITPAELYIQEKGTFKTTDQATLFSDV
GISAGSNSLGSLSVSADNTIKFEFTSGSLAASGSVGTITYTKTSTGWVCTTANLSTAKPSGCS

Nucleotide


Download         Length: 432 bp        

>NTDB_id=408930 GO175_RS02165 WP_154184015.1 353022..353453(+) (pilA) [Vibrio fluvialis strain F8658]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCCATCATCGGGGTCCT
GTCGGCGATTGCCGTACCCGCTTATAAAAACTATGTAGCCAAAAGTGAAGCTGCATCAGCGTTAGCAACTTTGAAGTCAC
TAATCACTCCAGCTGAGTTGTACATTCAAGAAAAAGGAACATTTAAGACAACTGACCAAGCTACGCTATTTTCAGATGTT
GGTATCTCTGCTGGCTCAAACAGTTTGGGTAGTTTGAGTGTTTCAGCTGATAACACCATTAAATTTGAGTTCACTTCTGG
ATCATTAGCAGCAAGTGGTTCAGTTGGAACAATAACTTATACAAAAACCTCAACTGGCTGGGTATGTACCACTGCAAACT
TAAGTACAGCTAAGCCAAGTGGCTGCTCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

46

100

0.483
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.241

100

0.434
  pilA Vibrio cholerae strain A1552

39.241

100

0.434
  pilA Vibrio cholerae C6706

39.241

100

0.434
  pilA Pseudomonas aeruginosa PAK

41.216

100

0.427
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.621

100

0.392
  pilA Acinetobacter baumannii strain A118

36.601

100

0.392
  pilA/pilAI Pseudomonas stutzeri DSM 10701

34.868

100

0.371

Multiple sequence alignment