Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GO175_RS02175 Genome accession   NZ_CP046855
Coordinates   355183..356409 (+) Length   408 a.a.
NCBI ID   WP_020332199.1    Uniprot ID   S7J7L5
Organism   Vibrio fluvialis strain F8658     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 350183..361409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GO175_RS02150 fldB 350673..351194 (+) 522 WP_020430452.1 flavodoxin FldB -
  GO175_RS02155 ampD 351199..351750 (-) 552 WP_158147899.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GO175_RS02160 nadC 351903..352790 (+) 888 WP_061056143.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GO175_RS02165 pilA 353022..353453 (+) 432 WP_154184015.1 pilin Machinery gene
  GO175_RS02170 pilB 353460..355147 (+) 1688 Protein_309 type IV-A pilus assembly ATPase PilB -
  GO175_RS02175 pilC 355183..356409 (+) 1227 WP_020332199.1 type II secretion system F family protein Machinery gene
  GO175_RS02180 pilD 356461..357330 (+) 870 WP_158147900.1 A24 family peptidase Machinery gene
  GO175_RS02185 coaE 357333..357941 (+) 609 WP_158147901.1 dephospho-CoA kinase -
  GO175_RS02190 zapD 357974..358714 (+) 741 WP_158147902.1 cell division protein ZapD -
  GO175_RS02195 yacG 358769..358966 (+) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  GO175_RS02200 parC 359017..361290 (-) 2274 WP_020332194.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44670.91 Da        Isoelectric Point: 10.5318

>NTDB_id=408931 GO175_RS02175 WP_020332199.1 355183..356409(+) (pilC) [Vibrio fluvialis strain F8658]
MKPTKGPELKNYRWKGVNSTGKKVAGQTLAISEIEVRDKLKEQHIQVKKIKKGSVSVLTRLTNRVKTKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKGVEAGTPISKAMRTASTHFDALYVDLIQTGEQSGNLAEVFERLATYRE
KSEQLRAKVIKALIYPSMVILVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQILKLSHGVQAYSAGAFIASLGLVFG
IKAARKKSFVVRLKTSQLSLRFPIIGGVLAKAAIAKFSRTLATSFSAGIPILASLKTTAKTAGNVHFETAIQDVHRHTAA
GMPMYIAMRNTQAFPEMVLQMVMIGEESGKLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGIVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=408931 GO175_RS02175 WP_020332199.1 355183..356409(+) (pilC) [Vibrio fluvialis strain F8658]
ATGAAACCGACCAAAGGCCCTGAGTTAAAAAACTATCGCTGGAAAGGCGTCAACAGCACTGGCAAGAAAGTCGCGGGACA
GACACTGGCTATCAGTGAAATAGAAGTCCGCGACAAACTGAAAGAACAGCACATTCAGGTTAAGAAGATCAAAAAAGGCA
GCGTGTCAGTTCTGACTCGTCTCACCAATCGGGTCAAAACTAAAGACATCACCATCCTAACCCGCCAGCTTGCCACCATG
CTGACCACCGGAGTGCCAATTGTGCAGGCACTTAAATTGGTCGGCGATAATCATCGTAAAGCCGAGATGAAATCGATTCT
GGCGCAAATTACCAAAGGCGTCGAAGCCGGTACGCCGATTTCCAAAGCGATGCGCACTGCCAGCACTCACTTTGACGCGT
TGTACGTCGACTTAATTCAGACTGGCGAGCAGTCCGGCAATCTTGCCGAAGTCTTTGAACGCCTTGCCACCTATCGGGAA
AAAAGTGAGCAACTGCGCGCGAAAGTAATCAAAGCGCTGATCTATCCCAGCATGGTCATTCTGGTGGCACTCGGCGTCTC
CTATTTGATGCTGACGATGGTCATTCCAGAGTTCGAAAGTATGTTCAAGGGTTTTGGGGCTGAACTGCCTTGGTTTACTC
AGCAGATCCTCAAGTTATCGCATGGGGTACAAGCCTACAGTGCGGGGGCGTTCATCGCGTCATTGGGCTTGGTGTTTGGC
ATCAAAGCAGCACGGAAAAAATCCTTCGTCGTCCGTCTTAAAACCAGCCAGCTCAGCCTGCGTTTTCCCATCATTGGCGG
TGTGCTTGCCAAAGCCGCAATTGCCAAATTCAGCCGTACTCTGGCGACCAGCTTCAGTGCGGGAATTCCGATACTCGCCA
GCCTCAAAACCACCGCTAAAACGGCAGGTAACGTGCATTTTGAAACCGCCATTCAGGATGTTCACCGCCATACGGCGGCA
GGGATGCCGATGTATATTGCAATGCGCAACACACAAGCCTTTCCGGAAATGGTGTTGCAAATGGTGATGATCGGCGAGGA
GTCCGGCAAGCTCGACGACATGCTCAATAAAGTCGCCACCATCTATGAGTTTGAAGTCGACAACACCGTCGACAATCTGG
GCAAAATTCTTGAACCGCTGATTATCGTATTTTTGGGCATTGTGGTCGGTGGATTGGTGGTGGCGATGTACCTGCCAATC
TTTAACTTAATGAGTGTATTAGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7J7L5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

87.01

100

0.87
  pilC Vibrio campbellii strain DS40M4

73.105

100

0.733
  pilC Acinetobacter baumannii D1279779

41.667

100

0.417
  pilC Legionella pneumophila strain ERS1305867

41.353

97.794

0.404
  pilC Acinetobacter baylyi ADP1

41.31

97.304

0.402
  pilC Pseudomonas stutzeri DSM 10701

40.152

97.059

0.39
  pilG Neisseria gonorrhoeae MS11

39.599

97.794

0.387
  pilG Neisseria meningitidis 44/76-A

39.098

97.794

0.382

Multiple sequence alignment