Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPY12_RS13745 Genome accession   NZ_CP046846
Coordinates   2984566..2986281 (-) Length   571 a.a.
NCBI ID   WP_199250769.1    Uniprot ID   -
Organism   Vibrio mimicus strain F9458     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2979566..2991281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY12_RS13725 - 2980316..2981149 (-) 834 WP_005524367.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  GPY12_RS13730 - 2981219..2982688 (-) 1470 WP_070381281.1 AAA family ATPase -
  GPY12_RS13735 aroB 2982742..2983827 (-) 1086 WP_187377492.1 3-dehydroquinate synthase -
  GPY12_RS13740 aroK 2983855..2984379 (-) 525 WP_000818614.1 shikimate kinase AroK -
  GPY12_RS13745 pilQ 2984566..2986281 (-) 1716 WP_199250769.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY12_RS13750 pilP 2986307..2986825 (-) 519 WP_158139643.1 pilus assembly protein PilP Machinery gene
  GPY12_RS13755 pilO 2986815..2987405 (-) 591 WP_000135848.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY12_RS13760 pilN 2987398..2987982 (-) 585 WP_000903043.1 PilN domain-containing protein Machinery gene
  GPY12_RS13765 pilM 2987972..2988988 (-) 1017 WP_158139644.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62395.43 Da        Isoelectric Point: 4.5760

>NTDB_id=408836 GPY12_RS13745 WP_199250769.1 2984566..2986281(-) (pilQ) [Vibrio mimicus strain F9458]
MRNGLKTYVAQTWLTLWAGLVLCASSVAISAESASANQLENIDFRVNKDKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVQSVEVFRKEPSTQLIATIEGEFKHDYTLKGKYLEVVISKLKADEKPQPKSILEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVSGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARMAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGTISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLDELGVRWGVMSTNGSHSVGGTIESNLWQQGLLAEDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SDTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATTVSFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=408836 GPY12_RS13745 WP_199250769.1 2984566..2986281(-) (pilQ) [Vibrio mimicus strain F9458]
ATGAGAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTATGGGCTGGCTTGGTACTTTGTGCTTCTTCCGT
GGCGATTAGCGCTGAATCAGCTTCCGCCAATCAACTAGAAAACATTGATTTTCGGGTCAATAAAGACAAAGCGGCTGTGT
TGATTGTCGAATTGGCTTCACCATCAGCGGTGGTGGATGTACAAAAAGTCCAAGAAGGGCTGAGTATTGAACTCCTGAAA
ACTGATGTAGCAGACGACAAACTCTATTTATTGGATGTGAAGGATTTCTCAACACCAGTACAAAGCGTAGAAGTCTTCCG
TAAAGAACCTAGTACCCAGCTTATCGCAACCATCGAAGGTGAATTTAAGCATGACTACACCTTAAAAGGGAAATACCTTG
AGGTGGTAATCAGTAAGCTCAAAGCAGACGAGAAGCCTCAGCCTAAAAGTATTTTGGAAAAAGAGGGCAAGCTGATCTCG
ATTAACTTCCAAGACATTCCAGTGCGTAACGTACTGCAGTTGATTGCTGATTATAACGGTTTCAACCTCGTTGTTTCAGA
TTCAGTCTCGGGCAATCTGACTTTGCGTTTGGATGGTGTACCTTGGCAGCAAGTGCTGGATATTATCCTGCAGGTTAAAG
GGCTTGATAAACGCGTTGATGGCAATGTAATCCTGATTGCGCCAAAAGAAGAGCTGGATTTGCGTGAGAAACAAGCGTTA
GAAAAAGCACGTATGGCAGAAGAATTAGGTGATTTAAAATCTGAAATCATCAAAATTAACTTTGCCAAAGCATCTGATAT
CGCAGCAATGATTGGTGGTGAAGGTAACGTCAATATGCTGTCTGAGCGTGGCACGATCAGTATTGATGAGCGTACTAACT
CACTGCTGATTCGCGAATTGCCCGATAATATCGCGGTGATCCGTGAAATCATCGAATCGCTAGATATTCCGGTCAAGCAA
GTCCAGATTGAAGCACGTATTGTCACCGTAAAAGAAGGTAACTTGGATGAGCTTGGTGTGCGTTGGGGTGTTATGTCAAC
CAACGGCAGCCACTCTGTGGGTGGAACAATTGAAAGTAACTTATGGCAACAAGGACTGCTGGCGGAAGATGAGTTTCCAG
TTGATGAATTTTTGAATGTTAACTTAGCTTCTACGTCCGCTAATGCCTCTTCTATCGCTTTCCAAGTTGCTAAGTTGGGC
TCAGATACCTTATTGGATTTGGAGTTATCTGCGTTACAAAATGAATCGAAGGCAGAAATCATTTCTAGCCCTCGTTTGAT
TACGACCAATAAACAGCCTGCGTATATTGAACAAGGTACTGAAATTCCTTATCTCGAATCTTCATCTAGTGGTGCAACAA
CCGTATCGTTCAAGAAAGCCGTGTTGAGCTTGAAAGTGACACCACAAATTACGCCTGATAATCGTTTAGTACTGGATTTG
AGTGTGACTCAGGACCGTCGTGGCGAAACGGTGAAAACAGGAACAGGTGAAGCGGTATCGATCGATACCCAGCGTATTGG
TACACAAGTTCTGGTCAATAATGGTGAAACTGTCGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCAGTGGATA
AGGTGCCGTTGCTAGGTGATCTCCCTGTTTTAGGTGCGTTGTTTAGACGAACTTACGAACAAATGGGCAAAAGTGAGCTG
CTGATTTTCGTCACACCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(181-229)

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

95.447

100

0.954
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

95.447

100

0.954
  pilQ Vibrio campbellii strain DS40M4

78.584

96.497

0.758
  pilQ Legionella pneumophila strain ERS1305867

43.173

94.921

0.41
  pilQ Legionella pneumophila strain Lp02

43.173

94.921

0.41
  comQ Acinetobacter baylyi ADP1

39.446

100

0.399
  pilQ Acinetobacter baumannii strain A118

38.33

100

0.394
  pilQ Acinetobacter baumannii D1279779

38.33

100

0.394
  pilQ Pseudomonas aeruginosa PAK

39.785

97.723

0.389

Multiple sequence alignment