Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   GPY12_RS13765 Genome accession   NZ_CP046846
Coordinates   2987972..2988988 (-) Length   338 a.a.
NCBI ID   WP_158139644.1    Uniprot ID   -
Organism   Vibrio mimicus strain F9458     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2982972..2993988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY12_RS13740 aroK 2983855..2984379 (-) 525 WP_000818614.1 shikimate kinase AroK -
  GPY12_RS13745 pilQ 2984566..2986281 (-) 1716 WP_199250769.1 type IV pilus secretin PilQ family protein Machinery gene
  GPY12_RS13750 pilP 2986307..2986825 (-) 519 WP_158139643.1 pilus assembly protein PilP Machinery gene
  GPY12_RS13755 pilO 2986815..2987405 (-) 591 WP_000135848.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY12_RS13760 pilN 2987398..2987982 (-) 585 WP_000903043.1 PilN domain-containing protein Machinery gene
  GPY12_RS13765 pilM 2987972..2988988 (-) 1017 WP_158139644.1 type IV pilus assembly protein PilM Machinery gene
  GPY12_RS13770 - 2989130..2991607 (+) 2478 WP_000668749.1 penicillin-binding protein 1A -
  GPY12_RS13775 oxyR 2991683..2992567 (-) 885 WP_158139645.1 DNA-binding transcriptional regulator OxyR -
  GPY12_RS13780 - 2992707..2993438 (+) 732 WP_000495220.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37563.22 Da        Isoelectric Point: 7.6008

>NTDB_id=408840 GPY12_RS13765 WP_158139644.1 2987972..2988988(-) (pilM) [Vibrio mimicus strain F9458]
MGKTLVTGIDIGHHSIKAVVLKPMGDTYALMGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDNA
VISKVLQIDSDLERREQEFAIYQAFSHQSPFPVEDLSLDFVKVAEKNIARSSTSTFQVYATKKEVVESRLQASKKAGFEP
ILMDVQVHSLLHLWQLATRAYRRPDWMLIDIGHTQSSLCLDFAEKMPFYKDVPLGTRILSAEASSTPALGDSLMQDPTQR
FVNEFVDKVARQIQLFTSVHGAQSLGGLWLSGGGATIAGLEEALHQRLSLPCEVLNPFSLFKTNVSRRKRQVADGQRFST
AAGLALRGLAWLESEHAA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=408840 GPY12_RS13765 WP_158139644.1 2987972..2988988(-) (pilM) [Vibrio mimicus strain F9458]
ATGGGTAAAACATTAGTTACGGGTATTGATATCGGCCACCACAGCATAAAAGCCGTAGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTATGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGGTTGCCCTTATTTAGTCACAAAGTCGCGATTGCAATTCCGGATAACGCT
GTAATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCGTCGAGAACAGGAATTTGCCATCTATCAGGCCTTCTCTCA
TCAATCTCCATTTCCCGTTGAAGATCTTAGCCTCGATTTCGTCAAAGTGGCGGAAAAAAATATCGCGCGCAGTAGTACAA
GCACCTTCCAAGTCTATGCCACGAAAAAAGAAGTAGTGGAAAGCCGTTTACAAGCGAGCAAAAAAGCGGGTTTTGAGCCG
ATTTTGATGGATGTGCAAGTTCATAGCTTGCTCCATTTATGGCAGCTTGCTACTCGTGCCTATCGACGTCCGGATTGGAT
GCTGATTGATATTGGGCATACCCAGAGTTCATTGTGCCTTGATTTCGCAGAAAAAATGCCTTTTTACAAAGATGTCCCAC
TGGGTACGCGAATATTGAGTGCGGAGGCGAGTAGTACTCCCGCCTTAGGCGATTCATTAATGCAGGATCCCACTCAGCGT
TTTGTTAATGAATTCGTGGATAAGGTTGCGCGTCAAATTCAGTTGTTTACTTCAGTGCATGGGGCGCAGAGTCTTGGCGG
CTTATGGCTTTCCGGCGGCGGTGCAACCATTGCTGGACTTGAGGAGGCGTTACACCAACGTTTATCACTTCCCTGTGAAG
TGTTGAATCCATTCTCACTATTCAAAACGAATGTCTCAAGACGCAAGCGCCAAGTGGCTGATGGGCAGCGCTTTAGCACT
GCCGCTGGCCTTGCATTGCGTGGTTTAGCATGGTTGGAGAGTGAACATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

85.207

100

0.852
  pilM Vibrio campbellii strain DS40M4

49.704

100

0.497

Multiple sequence alignment