Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GPY41_RS18420 Genome accession   NZ_CP046835
Coordinates   2251515..2253203 (+) Length   562 a.a.
NCBI ID   WP_158123814.1    Uniprot ID   -
Organism   Vibrio vulnificus strain 07-2444     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2246515..2258203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY41_RS18400 pdhR 2248181..2248948 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  GPY41_RS18405 ampD 2249337..2249909 (-) 573 WP_158123812.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  GPY41_RS18410 nadC 2250002..2250889 (+) 888 WP_158123813.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GPY41_RS18415 pilA 2251112..2251510 (+) 399 WP_130312477.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GPY41_RS18420 pilB 2251515..2253203 (+) 1689 WP_158123814.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GPY41_RS18425 pilC 2253252..2254478 (+) 1227 WP_017789875.1 type II secretion system F family protein Machinery gene
  GPY41_RS18430 pilD 2254572..2255441 (+) 870 WP_158123815.1 A24 family peptidase Machinery gene
  GPY41_RS18435 coaE 2255443..2256051 (+) 609 WP_158123816.1 dephospho-CoA kinase -
  GPY41_RS18440 zapD 2256084..2256824 (+) 741 WP_053541912.1 cell division protein ZapD -
  GPY41_RS18445 yacG 2256887..2257081 (+) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  GPY41_RS18450 rplS 2257314..2257667 (-) 354 WP_011079544.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62361.35 Da        Isoelectric Point: 5.6865

>NTDB_id=408633 GPY41_RS18420 WP_158123814.1 2251515..2253203(+) (pilB) [Vibrio vulnificus strain 07-2444]
MSTNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEKLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADSQELQAAIRRLYGKSIKQSQSSGLKEIHQDELA
SLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=408633 GPY41_RS18420 WP_158123814.1 2251515..2253203(+) (pilB) [Vibrio vulnificus strain 07-2444]
ATGTCAACCAACCTTGCCACGATTCTTCGTCAGGCCAATCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTCACTGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCTGATGAACTAACCGAAAAGCTAA
GTGCGATTTTTGGCTTACCACGCACAGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGCTTACGTGAG
CTGATCACTCGTCATCAAGCACTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCTACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAGGTGGAGATTGTTTTGGCTGATAGCCAAGAGCTGCAAGCGG
CGATTCGCAGACTCTATGGAAAGAGCATCAAACAGAGCCAAAGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
AGTTTGGTCGAGGTTTCAGATGACGAAGTCGAGTCAATTGAAGACTTAAGCCAAGATGAATCTCCCGTGAGCCGCTTTAT
TAACCAAATTCTGCTGGATGCGGTACGTAAAGGCGCGTCCGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTAGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCTGCAAGGATAAAG
ATCCTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGAAACCGC
GATTGATATGCGTGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGTA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTGCTTAACCGGCCAGAAGT
GAATATTTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGCCACCTTGTGCTATCCACCTTACATACCAACTCGGCAGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTAGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAACAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCGGCAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGTGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.69

100

0.767
  pilB Vibrio parahaemolyticus RIMD 2210633

75.979

100

0.76
  pilB Vibrio cholerae strain A1552

74.245

100

0.744
  pilB Legionella pneumophila strain ERS1305867

52.827

91.281

0.482
  pilB Acinetobacter baumannii D1279779

50.951

93.594

0.477
  pilB Acinetobacter baylyi ADP1

50.383

92.883

0.468
  pilF Neisseria gonorrhoeae MS11

45.487

100

0.457
  pilF Thermus thermophilus HB27

40.787

99.466

0.406
  pilB/pilB1 Synechocystis sp. PCC 6803

36.888

100

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363
  pilB Haemophilus influenzae Rd KW20

41.942

86.121

0.361

Multiple sequence alignment