Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GPY16_RS02150 Genome accession   NZ_CP046832
Coordinates   444131..445915 (+) Length   594 a.a.
NCBI ID   WP_017419660.1    Uniprot ID   A0A087IG05
Organism   Vibrio vulnificus strain 06-2410     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 439131..450915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPY16_RS02130 pilM 441445..442452 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  GPY16_RS02135 pilN 442442..443032 (+) 591 WP_045629546.1 PilN domain-containing protein Machinery gene
  GPY16_RS02140 pilO 443025..443615 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  GPY16_RS02145 pilP 443605..444117 (+) 513 WP_038940290.1 pilus assembly protein PilP Machinery gene
  GPY16_RS02150 pilQ 444131..445915 (+) 1785 WP_017419660.1 type IV pilus secretin PilQ Machinery gene
  GPY16_RS02155 aroK 446117..446635 (+) 519 WP_011079357.1 shikimate kinase AroK -
  GPY16_RS02160 aroB 446668..447759 (+) 1092 WP_011079358.1 3-dehydroquinate synthase -
  GPY16_RS02165 - 447810..449305 (+) 1496 Protein_398 AAA family ATPase -
  GPY16_RS02170 - 449384..450211 (+) 828 WP_017419662.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 64591.66 Da        Isoelectric Point: 4.5077

>NTDB_id=408579 GPY16_RS02150 WP_017419660.1 444131..445915(+) (pilQ) [Vibrio vulnificus strain 06-2410]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGDSGSGGSGDEGGSNSIDDF
LNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSF
KKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPL
LGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=408579 GPY16_RS02150 WP_017419660.1 444131..445915(+) (pilQ) [Vibrio vulnificus strain 06-2410]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACTTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAATGCAG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACCGTGGTGGATTTGAAACCAGTGGCAGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTTAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTTTCGACCGTGGTTGAAAG
TATCGAAGTATTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTCAGCGGTGAATTTCGTCACGACTATCGTCTTT
CTGGACGCTATCTCGAGGTGACAATTTCTCGCATCAAATCGGAGCAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGCTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAACGTTTTGCAGCTTATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCTGTCACTGGTAATGTGACGCTGAGATTGGATGGGGTACCTTGGCAGCAAGTGCTCGACATCA
TTCTTCAAGTGAAAGGTCTAGACAAACGTGTTGAAGGCAATGTTGTGCTCATTGCTCCTCAAGATGAACTGGATTTACGA
GAGAAGCAGCAGCTTGAGAAAGAACGTCTGGCGGAAGAGCTTGGCTCCCTTAAATCGGAAATCATCAAAGTCAACTTTGC
CAAAGCGTCAGACATTGCTGAGATGATCAATGGCAAAGAGTCGGTCAGCATGCTGTCAGAGCGTGGTAGTGTGGCTATTG
ATGAACGGACAAACTCTCTTTTATTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCACGCATTGTCACGGTGAATGAAGGCAATCTTGATGAGCTTGGTGTCCGTTG
GGGGTTCACCTCCATCAACGGCAGCACAACCGTCGGCGGTTCTATCGAAAACAACCTAGCAACCATTGGTCTCTACAATG
CAGGCAGTGGGAGTGGCAGTGGTGACTCAGGTAGCGGTGGCAGTGGTGATGAAGGCGGGTCGAACTCCATTGATGATTTC
CTCAATGTCAATCTTGCTGCGACTTCTGCAAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTT
GTTGGATCTCGAGCTATCAGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAACCAATA
AGAAGCCTGCTTATATCGAGCAAGGTACAGAGATTCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTTTCCTTT
AAGAAAGCTGTGCTTAGCCTGAAAGTGACCCCTCAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGTTACACA
AGATAGACCTGGCGAAATTGTCAAAACAGGTACTGGGGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCT
TGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATCTTCCAACACAGCATAACCAATACCACGGACAAGGTTCCTCTG
TTAGGTGATTTACCCTTATTGGGTGCGCTTTTCCGCCGTAGCTATGAAAACGTAGGCAAACGTGAATTGCTGATATTTGT
CACACCTAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Bacterial type II and III secretion system protein (Secretin)

(434-592)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A087IG05

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.966

99.327

0.774
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.068

99.327

0.736
  pilQ Vibrio cholerae strain A1552

74.068

99.327

0.736
  pilQ Legionella pneumophila strain Lp02

41.429

94.276

0.391
  pilQ Legionella pneumophila strain ERS1305867

41.429

94.276

0.391
  pilQ Pseudomonas aeruginosa PAK

38.435

96.801

0.372
  comQ Acinetobacter baylyi ADP1

36.824

99.663

0.367

Multiple sequence alignment